Model membrane approaches to determine the role of calcium for the antimicrobial activity of friulimicin

Reder-Christ, Katrin; Falkenstein-Paul, Hildegard; Klocek, Gabriela; Al-Kaddah, Saad; Bakowsky, Udo; Bendas, Gerd

doi:10.1016/j.ijantimicag.2010.11.024

Cited by 7 publications

(10 citation statements)

References 12 publications

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“…A similar observation was made with 100 M MX-2401 in the presence of increasing amounts of Ca 2ϩ , in the absence of lipid (data not shown). Such a moderate change was previously observed for the natural lipopeptide antibiotic friulimicin (23) and was interpreted as indicating that the addition of calcium stabilizes the secondary structure. However, in other examples in the literature where increases in ellipticity and shifts are observed with increasing concentrations of Ca 2ϩ , the degrees of secondary structural change have been quantified and are typically small, for example, on the order of 2% and 13% for chromogranin A (CGA) (1) and calmodulin (20), respectively.…”

supporting

confidence: 71%

Antimicrobial Properties of MX-2401, an Expanded-Spectrum Lipopeptide Active in the Presence of Lung Surfactant

Dugourd¹,

Yang

Elliott

et al. 2011

Antimicrob Agents Chemother

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supporting

confidence: 71%

Antimicrobial Properties of MX-2401, an Expanded-Spectrum Lipopeptide Active in the Presence of Lung Surfactant

Dugourd¹,

Yang

Elliott

et al. 2011

Antimicrob Agents Chemother

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“…38 PC is also a widespread constituent of the membranes of living cells, 39 therefore the interactions between DAP and PC would be the biomimetic route of the DAP interactions with cell membrane lipid bilayers. 40, 41 Figure 6a shows the DAP removal percentage and capacity of IXB at varying PC concentrations ranging from 0.5 mM to 4 mM (physiological concentration range from FaSSIF to FeSSIF). Increasing the concentration of PC slightly increases the DAP removal percentage and capacity of IXB, and at 4 mM of PC, a 10% enhancement in the removal capacity was obtained.…”

Section: Resultsmentioning

confidence: 99%

“…38 PC is also a widespread constituent of the membranes of living cells, 39 therefore the interactions between DAP and PC would be the biomimetic route of the DAP interactions with cell membrane lipid bilayers. 40,41 When the molar ratio of maleic acid carboxylate groups to the DAP carboxylate groups was identical (at ~10 mM of maleic acid), the DAP removal capacity of IXB reduced ~ 7 % compared with the DAP removal capacity of IXB in the absence of maleic acid. This 7 % reduction on the DAP removal capacity was found in Figure 6e in the presence of 10 mM maleic acid.…”

Section: Effects Of Sif Components On Ixb-mediated Dap Removalmentioning

confidence: 99%

Ion exchange biomaterials to capture daptomycin and prevent resistance evolution in off-target bacterial populations

Yeh

Narasimhalu

Steeg

et al. 2022

Preprint

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Daptomycin (DAP), a cyclic anionic lipopeptide antibiotic, is among the last resorts to treat multidrug resistant (vancomycin resistant Enterococcus faecium or methicillin resistant Staphylococcus aureus) Gram-positive bacterial infections. DAP is administered intravenously and biliary excretion results in the introduction of DAP (~5-10 % of the intravenous DAP dose) arriving in the gastrointestinal (GI) tract where it drives resistance evolution in off-target populations of Enterococcus faecium bacteria. Previously, we have shown that the oral administration of cholestyramine, an ion exchange biomaterial (IXB) sorbent, prevents DAP treatment from enriching DAP-resistance in populations of E. faecium shed from mice. Here, we engineer the biomaterial-DAP interfacial interactions to uncover the antibiotic removal mechanisms. The IXB-mediated DAP capture from aqueous media was measured in both controlled pH/electrolyte solutions and in simulated intestinal fluid (SIF) to uncover the molecular and colloidal mechanisms of DAP removal from the GI tract. Our findings show that the IXB electrostatically adsorbs the anionic antibiotic via a time-dependent diffusion-controlled process. Unsteady-state diffusion-adsorption mass balance describes the dynamics of adsorption well, and the maximum removal capacity is beyond the electric charge stoichiometric ratio because of DAP self-assembly. This study may open new opportunities for optimizing cholestyramine adjuvant therapy to prevent DAP resistance, as well as designing novel biomaterials to remove off-target antibiotics from the GI tract.

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“…Yet, the ability of QCM to analyze the peptide-peptide interaction in the presence of Ca 2+ was limited. Further techniques (atomic force microscopy and circular dichroism spectroscopy) were necessary to show that calcium influences the conformation of friulimicin thus enabling the peptide to bind to the membrane [55]. …”

Section: Biosensor Applications To Elucidate the Molecular Mode Of Acmentioning

confidence: 99%

Biosensor Applications in the Field of Antibiotic Research—A Review of Recent Developments

Reder-Christ

Bendas

2011

Sensors

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Antibacterials are among of the most important medications used in health care. However, their efficacy is increasingly impeded by a tremendous and globally spread bacterial resistance phenomenon. This bacterial resistance is accelerated by inadequate application of antibacterial drugs in humans, the widespread veterinary use of antibacterials, and antibacterial occurrence in the environment and food. Further, there is a lack of development of innovative novel drugs. Therefore, the search for novel antibacterials has to be intensified and the spread of antibacterials in the environment has to be restricted. Due to the fundamental progress in biosensor development and promising applications in the antibiotic field, this review gives for the first time an overview on the use and prospects of biosensor applications in that area. A number of reports have applied biosensors of different design and techniques to search for antibacterials in environmental and foodstuff matrices. These studies are discussed with respect to the analytical values and compared to conventional techniques. Furthermore, biosensor applications to elucidate the mode of action of antimicrobial drugs in vitro have been described. These studies were critically introduced referring to the informational value of those simulations. In summary, biosensors will be illustrated as an innovative and promising, although not yet comprehensively applied, technique in the antibacterial field.

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Model membrane approaches to determine the role of calcium for the antimicrobial activity of friulimicin

Cited by 7 publications

References 12 publications

Antimicrobial Properties of MX-2401, an Expanded-Spectrum Lipopeptide Active in the Presence of Lung Surfactant

Antimicrobial Properties of MX-2401, an Expanded-Spectrum Lipopeptide Active in the Presence of Lung Surfactant

Ion exchange biomaterials to capture daptomycin and prevent resistance evolution in off-target bacterial populations

Biosensor Applications in the Field of Antibiotic Research—A Review of Recent Developments

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