Biosensor Applications in the Field of Antibiotic Research—A Review of Recent Developments

Abstract

Antibacterials are among of the most important medications used in health care. However, their efficacy is increasingly impeded by a tremendous and globally spread bacterial resistance phenomenon. This bacterial resistance is accelerated by inadequate application of antibacterial drugs in humans, the widespread veterinary use of antibacterials, and antibacterial occurrence in the environment and food. Further, there is a lack of development of innovative novel drugs. Therefore, the search for novel antibacterials has to be intensified and the spread of antibacterials in the environment has to be restricted. Due to the fundamental progress in biosensor development and promising applications in the antibiotic field, this review gives for the first time an overview on the use and prospects of biosensor applications in that area. A number of reports have applied biosensors of different design and techniques to search for antibacterials in environmental and foodstuff matrices. These studies are discussed with respect to the analytical values and compared to conventional techniques. Furthermore, biosensor applications to elucidate the mode of action of antimicrobial drugs in vitro have been described. These studies were critically introduced referring to the informational value of those simulations. In summary, biosensors will be illustrated as an innovative and promising, although not yet comprehensively applied, technique in the antibacterial field.

High throughput LSPR and SERS analysis of aminoglycoside antibiotics

“…Therefore, there is a need to develop simple and rapid methods of analysing aminoglycoside antibiotics in order to provide novel methods for TDM, which could essentially enable the more widespread use of these extremely effective antibiotics. Given their low resistance rates in comparison to other classes of antibiotics, 7, 9 the clinical importance of aminoglycosides is being reassessed and the use of novel assays and instrumentation is one way in which these antibiotics could be widely reintroduced into the healthcare system while maintaining patient safety.…”

Development of a rapid low cost fluorescent biosensor for the detection of food contaminants

“…They can be developed using several types of transducer (RederͲChrist and Bendas, 2011) one of which is based on fluorescence either through quenching (Wang et al, 2011) or labelling of the biological recognition elements, e.g. fluorescently labelled antibody (Taitt et al, 2008).…”

Biosensors in Antimicrobial Drug Discovery: Since Biology until Screening Platforms

“…The first one employs immobilized aptamers (aptasensors) that are oligonucleic acids that interacts with the analyte of interest (protein, toxin) producing detectable signals. The second way for antimicrobial identification is the use of antibody biosensors [8,115]. Aptamers biosensors can be produced by SELEX process that select nucleic acid ligands specific of protein target [116].…”

“…The progress in biosensor development is a promising field of application in the antimicrobial research, as useful tool in the discovery of new antimicrobial compounds [8]. Also, biosensors can also be used to develop new diagnostic techniques more specific to detect the emergence of antimicrobial resistance in hospital environment and clinical samples [8][9][10][11]. As well as the analysis and detection of microbial food contaminants [12].…”