Mobilization and Biodegradation of 2-Methylnaphthalene by Amphiphilic Polyurethane Nano-Particle

Kim, Young–Bum; Kim, Ju‐Young; Kim, Eunki

doi:10.1007/s12010-009-8550-y

Cited by 8 publications

(2 citation statements)

References 26 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generally, besides sugars, various by-products so called “inhibitors” are inevitably generated during the dilute acid hydrolysis or pretreatment process [7] and these compounds could affect the growth and product formation of microorganisms during fermentation. The effect of inhibitors in lignocellulosic hydrolysates on the growth and lipid accumulation of oleaginous microorganisms has been studied by some researchers, however, most of these works mainly focused on the effect of aldehydes and organic acids [8], [9]. Also, in our previous studies on the influence of organic acids [10] and aldehydes [11], we found that these two kinds of compounds showed different effect on the growth and lipid accumulation of T. fermentans .…”

Section: Introductionmentioning

confidence: 92%

Effects of Alcohol Compounds on the Growth and Lipid Accumulation of Oleaginous Yeast Trichosporon fermentans

Huang

Liu

et al. 2012

PLoS ONE

View full text Add to dashboard Cite

The inhibitors present in dilute acid-treated lignocellulosic hydrolysates would show great effect on the growth and product formation of microorganisms. To understand their inhibitory law and mechanism on oleaginous microorganism could help improving the efficiency of lignocellulose hydrolysis, detoxification, and lipid fermentation. The effects of four representative alcohol compounds present in lignocellulosic hydrolysates, including furfuryl alcohol, vanillyl alcohol, catechol, hydroquinone on the cell growth and lipid accumulation of Trichosporon fermentans were systematically investigated in this work. The toxicity of selected alcohol compounds was well related to their log P value except furfuryl alcohol, whose log P value was the minimum but with the highest toxicity to T. fermentans. The inhibition of all the alcohol compounds on the growth of T. fermentans was more serious than on the lipid synthesis. Also, the growth of T. fermentans was more sensitive to the variation of inoculum size, temperature, and initial pH than lipid synthesis in the presence of alcohol compounds. Initial pH had more profound influence on the lipid fermentation than inoculum size and cultural temperature did. Careful control of fermentation conditions could be helpful for improving lipid yield of T. fermentans in lignocellulosic hydrolysates. Among the four alcohol compounds tested, most alcohol compounds showed inhibition on both sugar consumption and malic enzyme activity of T. fermentans. However, vanillyl alcohol had little influence on the malic enzyme activity. Similarly, all alcohol compounds except vanillyl alcohol exerted damage on the cell membrane of T. fermentans.

show abstract

Section: Introductionmentioning

confidence: 92%

Effects of Alcohol Compounds on the Growth and Lipid Accumulation of Oleaginous Yeast Trichosporon fermentans

Huang

Liu

et al. 2012

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Among the inhibitors, organic acids are generally the most abundant, and ten kinds of organic acids, including aliphatic acids (acetic acid, formic acid, levulinic acid and caproic acid), aromatic or furan acids (4-hydroxybenzoic acid, syringic acid, vanillic acid, furoic acid, ferulic acid and gallic acid) have been found in lignocellulosic hydrolysate. Little is known about their inhibition on lipid fermentation, however [16,17]. To provide some interesting information necessary for lipid fermentation on lignocellulosic hydrolysates, we systematically investigated, for the first time, the inhibitory effects of the above-mentioned organic acids on the growth and lipid accumulation of T. fermentans with a mixture of glucose and xylose at a ratio of 2:1 (wt/wt) as the carbon source, owing to its similarity to lignocellulosic hydrolysates.…”

Section: Introductionmentioning

confidence: 99%

Effect of organic acids on the growth and lipid accumulation of oleaginous yeast Trichosporon fermentans

Huang

Liu

et al. 2012

Biotechnol Biofuels

View full text Add to dashboard Cite

BackgroundMicrobial lipids have drawn increasing attention in recent years as promising raw materials for biodiesel production, and the use of lignocellulosic hydrolysates as carbon sources seems to be a feasible strategy for cost-effective lipid fermentation with oleaginous microorganisms on a large scale. During the hydrolysis of lignocellulosic materials with dilute acid, however, various kinds of inhibitors, especially large amounts of organic acids, will be produced, which substantially decrease the fermentability of lignocellulosic hydrolysates. To overcome the inhibitory effects of organic acids, it is critical to understand their impact on the growth and lipid accumulation of oleaginous microorganisms.ResultsIn our present work, we investigated for the first time the effect of ten representative organic acids in lignocellulosic hydrolysates on the growth and lipid accumulation of oleaginous yeast Trichosporon fermentans cells. In contrast to previous reports, we found that the toxicity of the organic acids to the cells was not directly related to their hydrophobicity. It is worth noting that most organic acids tested were less toxic than aldehydes to the cells, and some could even stimulate the growth and lipid accumulation at a low concentration. Unlike aldehydes, most binary combinations of organic acids exerted no synergistic inhibitory effects on lipid production. The presence of organic acids decelerated the consumption of glucose, whereas it influenced the utilization of xylose in a different and complicated way. In addition, all the organic acids tested, except furoic acid, inhibited the malic activity of T. fermentans. Furthermore, the inhibition of organic acids on cell growth was dependent more on inoculum size, temperature and initial pH than on lipid content.ConclusionsThis work provides some meaningful information about the effect of organic acid in lignocellulosic hydrolysates on the lipid production of oleaginous yeast, which is helpful for optimization of biomass hydrolysis processes, detoxified pretreatment of hydrolysates and lipid production using lignocellulosic materials.

show abstract

Microbial oil production from corncob acid hydrolysate by Trichosporon cutaneum

et al. 2012

View full text Add to dashboard Cite

Corncob was treated by dilute H(2)SO(4). The hydrolysate contained 45.7 g sugar/l. Without concentration or adding other nutrients, the hydrolysate, after being detoxified by overliming and adsorption with activated charcoal, was used for oil production using Trichosporon cutaneum. After 8 days' growth in shake-flasks, the biomass was 22.1 g/l with a lipid content of 36%. The lipid yield per mass of sugar was 17.4% (w/w). Corncob thus is a promising raw material for microbial oil production by this yeast.

show abstract

Mobilization and Biodegradation of 2-Methylnaphthalene by Amphiphilic Polyurethane Nano-Particle

Cited by 8 publications

References 26 publications

Effects of Alcohol Compounds on the Growth and Lipid Accumulation of Oleaginous Yeast Trichosporon fermentans

Effects of Alcohol Compounds on the Growth and Lipid Accumulation of Oleaginous Yeast Trichosporon fermentans

Effect of organic acids on the growth and lipid accumulation of oleaginous yeast Trichosporon fermentans

Microbial oil production from corncob acid hydrolysate by Trichosporon cutaneum

Contact Info

Product

Resources

About