Abstract:Corncob was treated by dilute H(2)SO(4). The hydrolysate contained 45.7 g sugar/l. Without concentration or adding other nutrients, the hydrolysate, after being detoxified by overliming and adsorption with activated charcoal, was used for oil production using Trichosporon cutaneum. After 8 days' growth in shake-flasks, the biomass was 22.1 g/l with a lipid content of 36%. The lipid yield per mass of sugar was 17.4% (w/w). Corncob thus is a promising raw material for microbial oil production by this yeast.
“…And this value increased when initial acetic acid concentration increased. This phenomenon was also observed in our previous work with other fermentation in lignocellulosic acid hydrolysates (acetic acid was present in it) [11,12]. The initial pH value of the medium containing acetic acid was adjusted to around 7.5 by NaOH before fermentation.…”
“…And this value increased when initial acetic acid concentration increased. This phenomenon was also observed in our previous work with other fermentation in lignocellulosic acid hydrolysates (acetic acid was present in it) [11,12]. The initial pH value of the medium containing acetic acid was adjusted to around 7.5 by NaOH before fermentation.…”
“…However, after 36 h of fermentation, the biomass of T. cutaneum maintained at its maximum point, indicating that its growth has entered into the stationary phase. While for lipid accumulation, the lipid content of T. cutaneum was low when compared with its fermentation on other low-cost substrates such as corncob hydrolysate [9]. It is possible that the carbon source concentration was low in ABE fermentation wastewater, and it is not enough for the lipid synthesis of T. cutaneum.…”
Section: Cell Growth and Lipid Accumulation Of T Cutaneum On Abe Fermentioning
confidence: 99%
“…In fact, before lipid extraction, the biomass of oleaginous yeast is usually dried by heating, and thus, the safety of using biomass produced by T. cutaneum as cattle feed might be fulfilled by this high-temperature sterilization. Also, considering the potential of using microbial oil produced by T. cutaneum for biodiesel production [9][10][11], the …”
Section: Comparison Of Fermentation With Other Oleaginous Microorganimentioning
confidence: 99%
“…Also belongs to genus Trichosporon, Trichosporon cutaneum is another oleaginous yeast that could accumulate microbial oil efficiently on various low-cost substrates [9][10][11]. More specially, this strain has high lipid yield and thus has great potential for biodiesel production [12].…”
In the present study, acetone-butanol-ethanol (ABE) fermentation wastewater with high chemical oxygen demand (COD) value (about 18,000 mg/L) was biologically treated by oleaginous yeast Trichosporon cutaneum without any pretreatment. During fermentation, most COD degradation was finished within 48 h and finally, a maximum COD degradation of 68% was obtained. The highest biomass and lipid content was 4.9 g/L and 14.7%, respectively. Various materials including sugars (glucose and xylose), organic acids (acetic acid and butyric acid), and alcohol compounds (ethanol and butanol) could be utilized as carbon sources by T. cutaneum simultaneously; thus, it has a broad carbon source spectrum and is a potential microorganism for biological treatment for various wastewaters. Overall, the lipid composition of microbial oils produced by this bioconversion is similar to that of vegetable oils, and thus, it could be used for biodiesel production.
“…Usually, the fatty acid composition of microbial lipid is similar to that of plant oils [4]. In addition, microbial lipids contain high fractions of polyunsaturated fatty acids and have the potential to serve as a source of significant quantities of transportation fuels [5].…”
In order to obtain lipid producing strain with high-yield, the wild type stain Rhodotorula glutinis was treated by low ion implantation, and optimization of fermentation medium for higher lipid yield was carried out using mutant strain. It was found that the strain had a higher positive mutation rate when the output power was 10 keV and the dose of N ? implantation was 80 9 2.6 9 10 13 ions/cm 2 . Then a high-yield mutant strain D30 was obtained through cid-heating coupling ultrasonic method and lipid yield was 3.10 g/L. Additionally, the surface response method was used to optimize fermentation medium. The three significant factors (glucose, peptone, KH 2 PO 4 ) were optimized using response surface methodology (RSM), and the optimized parameters of fermentation medium were as follows: glucose 73.40 g/L, peptone 1.06 g/L and KH 2 PO 4 3.56 g/L. Finally the fermentation characteristic of high-yield mutation strain D30 was studied, when fermentation time was 10 days, which lipid yield increased to 7.81 g/L. Fatty acid composition of the lipid was determined by GC, and the most represented fatty acids of mutant D30 were C16:0 (11.4 %), C16:1 (5.66 %), C18:1 (49.3 %), and C18:2 (27.0 %).
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