Beneficial Effect of Acetic Acid on the Xylose Utilization and Bacterial Cellulose Production by Gluconacetobacter xylinus

The possibility of using acetone-butanol-ethanol (ABE) fermentation wastewater for bacterial cellulose (BC) production by Gluconacetobacter xylinus was evaluated in this study. This is the first time that ABE fermentation wastewater was used as substrate for BC fermentation. The results provide detail information of metabolism of G. xylinus in ABE fermentation wastewater and the influence of wastewater environment on the structure of BC samples. Overall, this bioconversion could reduce the cost of BC production greatly.

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“…In our previous study, acetic acid has been proved to be one carbon source that could stimulate BC accumulation (Yang et al . ).…”

Section: Resultsmentioning

confidence: 97%

“…Interestingly, after the 6th day, the consumption of organic acids by G. xylinus became faster. In our previous study, acetic acid has been proved to be one carbon source that could stimulate BC accumulation (Yang et al 2014).…”

Section: Metabolism Of Gluconacetobacter Xylinus In Abe Fermentation mentioning

confidence: 99%

Evaluating the possibility of using acetone-butanol-ethanol (ABE) fermentation wastewater for bacterial cellulose production by Gluconacetobacter xylinus

Huang

Yang

Xiong

et al. 2015

Lett Appl Microbiol

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“…In our study, all used carbon sources were sugars except for acetate and ethanol, which are well-known substrates responsible for enhancing ATP production and inhibiting the anti-BC production processes [17]. The studies focusing on acetate and (or) ethanol addition and their consequent effects on BC production have been well documented [17,18,19,20]. Although some studies have tried to produce BC using acetate or ethanol as a sole carbon source, the BC yields are much lower than for sugars [16,36].…”

Section: Resultsmentioning

confidence: 99%

“…More often, both ethanol and acetate are spiked in media to enhance BC production by improving adenosine triphosphate (ATP) production, which is responsible for energy supply in the tricarboxylic acid (TCA) cycle and sugar metabolisms. Specifically, these processes are achieved by promoting the activities of glucokinase and fructokinase for BC production and inhibiting the activities of gluconokinase and glucose-6-phosphate dehydrogenase in pentose phosphate metabolism for energy production [17,18,19,20,21,22].…”

Section: Introductionmentioning

confidence: 99%

Insights into Bacterial Cellulose Biosynthesis from Different Carbon Sources and the Associated Biochemical Transformation Pathways in Komagataeibacter sp. W1

et al. 2018

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Cellulose is the most abundant and widely used biopolymer on earth and can be produced by both plants and micro-organisms. Among bacterial cellulose (BC)-producing bacteria, the strains in genus Komagataeibacter have attracted wide attention due to their particular ability in furthering BC production. Our previous study reported a new strain of genus Komagataeibacter from a vinegar factory. To evaluate its capacity for BC production from different carbon sources, the present study subjected the strain to media spiked with 2% acetate, ethanol, fructose, glucose, lactose, mannitol or sucrose. Then the BC productivity, BC characteristics and biochemical transformation pathways of various carbon sources were fully investigated. After 14 days of incubation, strain W1 produced 0.040–1.529 g L−1 BC, the highest yield being observed in fructose. Unlike BC yields, the morphology and microfibrils of BCs from different carbon sources were similar, with an average diameter of 35–50 nm. X-ray diffraction analysis showed that all membranes produced from various carbon sources had 1–3 typical diffraction peaks, and the highest crystallinity (i.e., 90%) was found for BC produced from mannitol. Similarly, several typical spectra bands obtained by Fourier transform infrared spectroscopy were similar for the BCs produced from different carbon sources, as was the Iα fraction. The genome annotation and Kyoto Encyclopedia of Genes and Genomes analysis revealed that the biochemical transformation pathways associated with the utilization of and BC production from fructose, glucose, glycerol, and mannitol were found in strain W1, but this was not the case for other carbon sources. Our data provides suggestions for further investigations of strain W1 to produce BC by using low molecular weight sugars and gives clues to understand how this strain produces BC based on metabolic pathway analysis.

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“…One possible reason for the increase could be the addition of acetic acid that was used for dissolving chitosan, which could be utilized as a carbon source for G. xylinus . Previous studies have shown that acetic acid can enhance BNC yield (Yang et al, 2014 ). However, the cellulose production was much lower after adding chitosan than that in the chitosan-free culture medium (1.46 g/L), indicating a serious inhibiting effect on cellulose production under static culture condition.…”

Section: Resultsmentioning

confidence: 97%

Using In situ Dynamic Cultures to Rapidly Biofabricate Fabric-Reinforced Composites of Chitosan/Bacterial Nanocellulose for Antibacterial Wound Dressings

et al. 2016

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Bacterial nano-cellulose (BNC) is considered to possess incredible potential in biomedical applications due to its innate unrivaled nano-fibrillar structure and versatile properties. However, its use is largely restricted by inefficient production and by insufficient strength when it is in a highly swollen state. In this study, a fabric skeleton reinforced chitosan (CS)/BNC hydrogel with high mechanical reliability and antibacterial activity was fabricated by using an efficient dynamic culture that could reserve the nano-fibrillar structure. By adding CS in culture media to 0.25–0.75% (w/v) during bacterial cultivation, the CS/BNC composite hydrogel was biosynthesized in situ on a rotating drum composed of fabrics. With the proposed method, BNC biosynthesis became less sensitive to the adverse antibacterial effects of CS and the production time of the composite hydrogel with desirable thickness could be halved from 10 to 5 days as compared to the conventional static cultures. Although, its concentration was low in the medium, CS accounted for more than 38% of the CS/BNC dry weight. FE-SEM observation confirmed conservation of the nano-fibrillar networks and covering of CS on BNC. ATR-FTIR showed a decrease in the degree of intra-molecular hydrogen bonding and water absorption capacity was improved after compositing with CS. The fabric-reinforced CS/BNC composite exhibited bacteriostatic properties against Escherichia coli and Staphylococcus aureus and significantly improved mechanical properties as compared to the BNC sheets from static culture. In summary, the fabric-reinforced CS/BNC composite constitutes a desired candidate for advanced wound dressings. From another perspective, coating of BNC or CS/BNC could upgrade the conventional wound dressings made of cotton gauze to reduce pain during wound healing, especially for burn patients.

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Beneficial Effect of Acetic Acid on the Xylose Utilization and Bacterial Cellulose Production by Gluconacetobacter xylinus

Abstract: In this work,

Cited by 19 publications

References 13 publications

Evaluating the possibility of using acetone-butanol-ethanol (ABE) fermentation wastewater for bacterial cellulose production by Gluconacetobacter xylinus

Evaluating the possibility of using acetone-butanol-ethanol (ABE) fermentation wastewater for bacterial cellulose production by Gluconacetobacter xylinus

Insights into Bacterial Cellulose Biosynthesis from Different Carbon Sources and the Associated Biochemical Transformation Pathways in Komagataeibacter sp. W1

Using In situ Dynamic Cultures to Rapidly Biofabricate Fabric-Reinforced Composites of Chitosan/Bacterial Nanocellulose for Antibacterial Wound Dressings

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