Effect of organic acids on the growth and lipid accumulation of oleaginous yeast Trichosporon fermentans

Huang, Chao; Wu, Hong; Liu, Zongjun; Cai, Jun; Lou, Wen‐Yong; Zong, Min‐Hua

doi:10.1186/1754-6834-5-4

Cited by 84 publications

(66 citation statements)

References 37 publications

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“…The authors are not aware of a comparable published study. There have been reports of the effects of selected inhibitors on growth and lipid production by selected yeast species, such as the effect of several inhibitors on Rhodosporidium toruloides [13], aldehydes and organic acids on Trichosporon fermentans [16,17] several inhibitors on Cryptococcus curvatus [50], and screening of the effect of several inhibitors on growth and lipid production by five oleaginous yeast species: R. glutinis, T. cutaneum, R. rubra, R. toruloides, and L. starkeyi [6]. These studies demonstrated that in addition to inhibiting growth, presence of inhibitors correlated with decreased cellular lipid content.…”

Section: Discussionmentioning

confidence: 99%

Carbon source utilization and inhibitor tolerance of 45 oleaginous yeast species

Sitepu

Selby

Lin

et al. 2014

Journal of Industrial Microbiology and Biotechnology

119

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Conversion of lignocellulosic hydrolysates to lipids using oleaginous (high lipid) yeasts requires alignment of the hydrolysate composition with the characteristics of the yeast strain, including ability to utilize certain nutrients, ability to grow independently of costly nutrients such as vitamins, and ability to tolerate inhibitors. Some combination of these characteristics may be present in wild strains. In this study, 48 oleaginous yeast strains belonging to 45 species were tested for ability to utilize carbon sources associated with lignocellulosic hydrolysates, tolerate inhibitors, and grow in medium without supplemented vitamins. Some well-studied oleaginous yeast species, as well as some that have not been frequently utilized in research or industrial production, emerged as promising candidates for industrial use due to ability to utilize many carbon sources, including Cryptococcus aureus, Cryptococcus laurentii, Hanaella aff. zeae, Tremella encephala, and Trichosporon coremiiforme. Other species excelled in inhibitor tolerance, including Candida aff. tropicalis, Cyberlindnera jadinii, Metschnikowia pulcherrima Schwanniomyces occidentalis and Wickerhamomyces ciferii. No yeast tested could utilize all carbon sources and tolerate all inhibitors tested. These results indicate that yeast strains should be selected based on characteristics compatible with the composition of the targeted hydrolysate. Other factors to consider include the production of valuable co-products such as carotenoids, availability of genetic tools, biosafety level, and flocculation of the yeast strain. The data generated in this study will aid in aligning yeasts with compatible hydrolysates for conversion of carbohydrates to lipids to be used for biofuels and other oleochemicals.

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Section: Discussionmentioning

confidence: 99%

Carbon source utilization and inhibitor tolerance of 45 oleaginous yeast species

Sitepu

Selby

Lin

et al. 2014

Journal of Industrial Microbiology and Biotechnology

119

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“…Their detoxification strategy involving extraction with ethyl acetate followed by hydrolysis and detoxification with activated carbon became the basic scheme of detoxification for many studies after that. Fermentation of sugar-based substrates, such as glucose, sucrose, fructose, lactose, whey, and xylose have been widely studied; however, research on lipid production from organic acids is still very limited [26,28,53,70]. In another study, Lian et al [100] investigated the fermentation of carboxylic acid present in the aqueous fraction of pyrolysis oil as a fermentation substrate for oleaginous yeasts to produce lipids.…”

Section: Indirect Fermentation Of Pyrolytic Sugarsmentioning

confidence: 97%

Microbial conversion of pyrolytic products to biofuels: a novel and sustainable approach toward second-generation biofuels

Islam

Hassan

et al. 2015

Journal of Industrial Microbiology and Biotechnology

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This review highlights the potential of the pyrolysis-based biofuels production, bio-ethanol in particular, and lipid in general as an alternative and sustainable solution for the rising environmental concerns and rapidly depleting natural fuel resources. Levoglucosan (1,6-anhydrous-β-D-glucopyranose) is the major anhydrosugar compound resulting from the degradation of cellulose during the fast pyrolysis process of biomass and thus the most attractive fermentation substrate in the bio-oil. The challenges for pyrolysis-based biorefineries are the inefficient detoxification strategies, and the lack of naturally available efficient and suitable fermentation organisms that could ferment the levoglucosan directly into bio-ethanol. In case of indirect fermentation, acid hydrolysis is used to convert levoglucosan into glucose and subsequently to ethanol and lipids via fermentation biocatalysts, however the presence of fermentation inhibitors poses a big hurdle to successful fermentation relative to pure glucose. Among the detoxification strategies studied so far, over-liming, extraction with solvents like (n-butanol, ethyl acetate), and activated carbon seem very promising, but still further research is required for the optimization of existing detoxification strategies as well as developing new ones. In order to make the pyrolysis-based biofuel production a more efficient as well as cost-effective process, direct fermentation of pyrolysis oil-associated fermentable sugars, especially levoglucosan is highlly desirable. This can be achieved either by expanding the search to identify naturally available direct levoglusoan utilizers or modify the existing fermentation biocatalysts (yeasts and bacteria) with direct levoglucosan pathway coupled with tolerance engineering could significantly improve the overall performance of these microorganisms.

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“…Although there have been many works investigating the inhibitory effect of acetic acid on ethanologenic yeasts under anaerobic conditions (Pampulha et al 1989;Bellissimi et al 2009;Casey et al 2010), so far only a few reports have referred to the effect of acetic acid on oleaginous microorganisms under aerobic conditions (Chen et al 2009;Hu et al 2009;Huang et al 2012). Therefore, little is known about this acid's inhibitory mechanism.…”

Section: Introductionmentioning

confidence: 99%

Effects of Acetic Acid and pH on the Growth and Lipid Accumulation of the Oleaginous Yeast Trichosporon fermentans

Liu

Wen

et al. 2015

BioResources

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Acetic acid, one major inhibitor released during the hydrolysis of lignocellulosic biomass, can be utilized by the oleaginous yeast Trichosporon fermentans without glucose repression. The effect of acetic acid on the cell growth and lipid accumulation of T. fermentans under controlled pH conditions was investigated in a 5-L fermentor. Undissociated acetic acid with concentrations of 0.026, 0.052, and 0.096 g L -1 in media contributed to approximately 12-, 24-, and 48-h lag phases, respectively, indicating that undissociated acetic acid is the inhibitory molecular form. The inhibition of cell growth was correlated with undissociated acetic acid concentration. However, acetic acid had little influence on the lipid accumulation of T. fermentans at different pH conditions. The specific glucose consumption rate decreased with increasing acetic acid concentration, but the impact of acetic acid on the specific xylose consumption rate was not pronounced. In addition, the variation of pH and acetic acid concentration had no significant influence on the fatty acid composition of the lipids. Acetic acid showed more severe inhibition under low pH conditions. The reduction of intracellular pH partly explains this inhibitory effect.

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Effect of organic acids on the growth and lipid accumulation of oleaginous yeast Trichosporon fermentans

Cited by 84 publications

References 37 publications

Carbon source utilization and inhibitor tolerance of 45 oleaginous yeast species

Carbon source utilization and inhibitor tolerance of 45 oleaginous yeast species

Microbial conversion of pyrolytic products to biofuels: a novel and sustainable approach toward second-generation biofuels

Effects of Acetic Acid and pH on the Growth and Lipid Accumulation of the Oleaginous Yeast Trichosporon fermentans

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