MMP2 and MMP9 are Associated with Apical Periodontitis Progression and Might be Modulated by TLR2 and MyD88

Barreiros, Driely; Filho, Paulo Nelson; Paula-Silva, Francisco Wanderley Garcia; Oliveira, Katharina Morant Holanda de; Lucisano, Marília Pacifico; Rossi, Andiara De; Silva, Léa Assed Bezerra; Küchler, Érika Calvano; Silva, Léa Assed Bezerra da

doi:10.1590/0103-6440201801731

Cited by 23 publications

(24 citation statements)

References 26 publications

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“…The increase levels of glucose in the blood and changes in inflammatory molecules cause alterations in the periodontal tissue [36], thereby modulating the activation of different kinases and proteases involved in the development of periodontitis [16]. Recent studies have demonstrated an association between MMP-2 and periodontitis in patients without diabetes [9, 11, 12, 19, 39]. Nevertheless, the relationship between periodontitis and the gelatinolytic activity of MMP-2 in T2DM patients is contradictory [23, 24].…”

Section: Discussionmentioning

confidence: 99%

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MMP-2 salivary activity in type 2 diabetes mellitus patients

Arreguín-Cano

Ayerdi-Nájera

Tacuba-Saavedra

et al. 2019

Diabetol Metab Syndr

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BackgroundType 2 diabetes mellitus (T2DM) and periodontitis are chronic inflammatory diseases with a bidirectional relationship. The uncontrolled levels of glucose in T2DM patients change the pathophysiology and balance of inflammatory mediators. Matrix Metalloproteinase-2 (MMP-2) is a zinc-dependent endopeptidase that is responsible for tissue remodeling and degradation of the extracellular matrix in periodontal tissue. Therefore, the uncontrolled levels of glucose in T2DM could lead to an imbalance in MMP-2 activity in saliva, favoring the development of periodontitis.MethodsNinety-seven T2DM patients from Hospital Dr. Donato Alarcon were included in the study. Following clinical examination, the patients were classified into four groups according to the presence and degree of periodontal disease and glycemic control. Blood and whole saliva samples (WSS) were collected from each patient. Blood samples were used for Hba1c and polymorphonuclear cells count determination, while WSS were used to determine MMP-2 activity, TIMP-1 and nitrite. MMP-2 activity was determined by zymography. TIMP-1 were determined by Western blotting, and nitric oxide (NO) levels were determined by the Griess method.ResultsOf the 97 patients with T2DM, 66 had periodontitis of different severities: 18 patients had mild periodontitis, 15 had moderate and 33 had severe. Salivary MMP-2 activity, HbA1c and TIMP-1 were positively correlated with the severity of periodontitis. On the other hand, the increase in HbA1c was negatively correlated with MMP-2 activity and quantity of TIMP-1 but was positively correlated with nitrite levels.ConclusionsT2DM with glycemic uncontrol conditions, distinct clinical alterations in periodontal tissue were identified, including a decrease in the gingival redness, increased the clinical attachment loss and imbalance of MMP-2/TIMP-1, as the possible causes of disorders promoting the progression of periodontitis. Accelerated periodontitis development with poor glycemic uncontrol likely results from the altered response of host defenses and decreased activity of polymorphonuclear cells. Taken together, these findings identify MMP-2 as a promising molecular market for periodontitis.

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Section: Discussionmentioning

confidence: 99%

“…During periodontal tissue inflammation, overexpression of MMPs leads to an increase in MMP levels in biological fluids such as saliva and gingival crevicular fluid [18, 19]. Previous studies on this topic have found higher levels of MMP-2 in saliva from patients with periodontitis [20, 21].…”

Section: Introductionmentioning

confidence: 99%

MMP-2 salivary activity in type 2 diabetes mellitus patients

Arreguín-Cano

Ayerdi-Nájera

Tacuba-Saavedra

et al. 2019

Diabetol Metab Syndr

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“…It is highlighted that the present findings must be exercised with caution and not transferred directly to clinical conditions because of obvious limitations of immunohistochemical analysis and studies in animals. Regarding the semi-quantitative analysis, the score system was used as in several previous published studies (15,30,31). Also, most of evaluated molecules (ALP and OPN) are extracellular proteins, making it difficult to perform a counting of immunolabelled cells.…”

Section: Endovac In Periapical Lesion Repairmentioning

confidence: 99%

Negative Pressure Irrigation Presents Mineralizing Potential in Dogs’ Immature Teeth with Periapical Lesion

et al. 2020

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The objective of this in vivo study was to assess the effect of the root canal irrigation by negative and positive apical pressure on the expression of molecules that are an indicative of cell differentiation with mineralizing phenotype in teeth of dogs with incomplete rhizogenesis and induced periapical lesion. A total of 30 teeth (60 roots) were distributed into 3 groups (n=20): EndoVac®, Conventional and Control. After 90 days, the routine histotechnical procedures were performed and the sections were submitted to immunohistochemical technique for the staining of osteopontin (OPN), alkaline phosphatase (ALP) and the RUNX2 transcription factor in the apical and periapical regions of the roots. A semi-quantitative analysis of the positive immunostaining was performed and the intensity of the expression was classified in absent (0), mild (1), moderate (2), or intense (3). Scores data were statistically analyzed by the Kruskal-Wallis non-parametric test and Dunn post-test, and the significance level was set at 5%. RUNX2 immunostaining revealed that in the negative pressure group there was a significantly stronger (p<0.05) immunostaining in comparison to the control group. Regarding the OPN expression, it was not possible to detect a statistically significant difference between the groups (p>0.05). After analyzing ALP immunostaining, a statistically significant difference was observed between the groups (p<0.05), and the negative pressure group showed a markedly stronger mark immunostaining than the control group. The results of the present in vivo study allowed concluding that negative apical pressure irrigation presents mineralizing potential in immature teeth with apical periodontitis.

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“…Control slides in which the primary antibody was omitted were used to test the specificity of immunostaining. Positive staining in periapical tissues was scored (1= mild, 2= moderate, and 3= intense) as previously described (23). For this analysis, all slides were prepared in the same batch to obtain a standardized staining and were evaluated by an experienced blind examiner.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Radiographic and Immunohistochemical Evaluation of Root Canal Treatment Using Different Irrigation Systems

et al. 2019

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The aim of this study was to evaluate the radiographic periapical repair and the synthesis of inflammatory mediators after endodontic treatment in a single session, using different irrigation protocols, in teeth with apical periodontitis. Experimental apical periodontitis were induced in dog’s teeth randomly assigned into 4 groups: G1 - Irrigation by Negative Apical Pressure (n= 20); G2 - Passive Ultrasonic Irrigation (n= 20), G3 - Positive Pressure Irrigation (n= 20); G4 - apical periodontitis without treatment (n= 20). After 180 days, the animals were euthanized, the tissues removed and submitted to histotechnical processing for immunohistochemical analysis of osteopontin (OPN), tumor necrosis factor-a (TNF-a) and interleukin 1-a (IL-1a). Radiographic analysis was performed using the Periapical Index (PAI), obtained prior to and 180 days following endodontic treatment. Data were analyzed using Wilcoxon signed-rank test, Fisher’s Exact test or Kruskal-Wallis test and Dunn’s post-test (a = 5%). Radiographically, after endodontic treatment, apical periodontitis persisted in 35% of G1 specimens, 40% of G2 and 40% of G3 (p>0.05), although a PAI reduction was observed (p<0.05). By immunohistochemical evaluation, endodontic treatment resulted in lower synthesis of TNF-a and OPN in periapical region, compared to apical periodontitis without treatment (p<0.05). Production of IL-1 was not modulated by endodontic treatment (p>0.05). Periapical healing was observed in approximately 60% of the cases after endodontic treatment performed in a single session with lower synthesis of TNF-a and OPN in the periapical region, regardless of the irrigation protocol used.

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MMP2 and MMP9 are Associated with Apical Periodontitis Progression and Might be Modulated by TLR2 and MyD88

Cited by 23 publications

References 26 publications

MMP-2 salivary activity in type 2 diabetes mellitus patients

MMP-2 salivary activity in type 2 diabetes mellitus patients

Negative Pressure Irrigation Presents Mineralizing Potential in Dogs’ Immature Teeth with Periapical Lesion

Radiographic and Immunohistochemical Evaluation of Root Canal Treatment Using Different Irrigation Systems

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