Negative Pressure Irrigation Presents Mineralizing Potential in Dogs’ Immature Teeth with Periapical Lesion

Abstract

The objective of this in vivo study was to assess the effect of the root canal irrigation by negative and positive apical pressure on the expression of molecules that are an indicative of cell differentiation with mineralizing phenotype in teeth of dogs with incomplete rhizogenesis and induced periapical lesion. A total of 30 teeth (60 roots) were distributed into 3 groups (n=20): EndoVac®, Conventional and Control. After 90 days, the routine histotechnical procedures were performed and the sections were submitted to immunohistochemical technique for the staining of osteopontin (OPN), alkaline phosphatase (ALP) and the RUNX2 transcription factor in the apical and periapical regions of the roots. A semi-quantitative analysis of the positive immunostaining was performed and the intensity of the expression was classified in absent (0), mild (1), moderate (2), or intense (3). Scores data were statistically analyzed by the Kruskal-Wallis non-parametric test and Dunn post-test, and the significance level was set at 5%. RUNX2 immunostaining revealed that in the negative pressure group there was a significantly stronger (p<0.05) immunostaining in comparison to the control group. Regarding the OPN expression, it was not possible to detect a statistically significant difference between the groups (p>0.05). After analyzing ALP immunostaining, a statistically significant difference was observed between the groups (p<0.05), and the negative pressure group showed a markedly stronger mark immunostaining than the control group. The results of the present in vivo study allowed concluding that negative apical pressure irrigation presents mineralizing potential in immature teeth with apical periodontitis.

Probiótico: avaliação da atividade antimicrobiana in vitro e do efeito in vivo na progressão de lesões periapicais, por meio de análise microscópica

“…Evidências científicas demostram efeitos benéficos no uso de probióticos em diferentes processos na cavidade bucal, variando o tipo de cepa probiótica e consequentemente o efeito nos diversos estudos, como no tratamento da periodontite (Ricoldi et al, 2017;Morales et al, 2018;Schmitter et al, 2018;Stanisic et al, 2020), prevenção e tratamento de mucosites e peri-implantite (Galofré et al, 2018;Silva et al, 2020), efeito na reabsorção óssea durante o movimento ortodôntico, prevenção de manchas brancas em pacientes ortodônticos (Pazzini et al,2017;Khoroushi e Kachui, 2017;Alp e Baka, 2018), efeito profilático contra carcinoma de células escamosas (Aghazadeh et al, 2017;La Rosa et al, 2020), além da atuação na prevenção de lesões de cárie (Allaker e Stephen, 2017;Kumar et al, 2018;Lin et al, 2018;Villavicencio et al, 2018;Angarita-Díaz et al, 2020;Bustamante et al, 2020). Revisões sistemáticas da literatura destacam claras evidências dos benefícios obtidos após a utilização de probióticos como adjuvantes no tratamento periodontal (Gruner et al, 2016;Martin-Cabezas et al, 2016;Matsubara et al, 2016;Ikram et al, 2018;Vives-Soler et al, 2020) Cerbo et al, 2016;Sunmola et al, 2019).…”