Abstract:There is evidence for the beneficial effects of forage peanut on the nutritive value and fermentation profile of silages; however, its effects on sugarcane silage have not been determined. The objective of the study was to evaluate the chemical composition, fermentation profile, microbial composition, and dry matter recovery (DMR) of sugarcane silage containing various amounts of forage peanut (Arachis pintoi cv. Belmonte) (0%, 25%, 50%, and 75% on a fresh matter basis), treated or untreated with Lactobacillus… Show more
“…In contrast, L. buchneri, a heterofermentative LAB, enhances silage's aerobic stability by converting LA into acetic acid (AA), thereby inhibiting filamentous fungi growth (Yin et al, 2023). da Costa et al (2022) reported that L. buchneri inoculation increased AA concentration and reduced yeast and ethanol content in sugarcane and peanut silages. Current research on whole-plant corn-soybean predominantly focuses on the intercropping model and the corn-soybean ratio during silage processing (Kizilsimsek et al, 2017;Ni et al, 2018;Zeng et al, 2020;Meng et al, 2022), and the above two mixed silage methods are also limited by nutrient loss and incomplete fermentation.…”
The mixture of whole-plant soybean and whole-plant corn silage (WPSCS) is nutrient balanced and is also a promising roughage for ruminants. However, few studies have investigated the changes in bacterial community succession in WPSCS inoculated with homofermentative and heterofermentative lactic acid bacteria (LAB) and whether WPSCS inoculated with LAB can improve fermentation quality by reducing nutrient losses. This study investigated the effect of Lactobacillus plantarum (L. plantarum) or Lactobacillus buchneri (L. buchneri) on the fermentation quality, aerobic stability, and bacterial community of WPSCS. A 40:60 ratio of whole-plant soybean corn was inoculated without (CK) or with L. plantarum (LP), L. buchneri (LB), and a mixture of LP and LB (LPB), and fermented for 14, 28, and 56 days, followed by 7 days of aerobic exposure. The 56-day silage results indicated that the dry matter content of the LP and LB groups reached 37.36 and 36.67%, respectively, which was much greater than that of the CK group (36.05%). The pH values of the LP, LB, and LPB groups were significantly lower than those of the CK group (p < 0.05). The ammoniacal nitrogen content of LB was significantly lower than that of the other three groups (p < 0.05), and the ammoniacal nitrogen content of LP and LPB was significantly lower than that of CK (p < 0.05). The acetic acid content and aerobic stability of the LB group were significantly greater than those of the CK, LP, and LPB groups (p < 0.05). High-throughput sequencing revealed a dominant bacteria shift from Proteobacteria in fresh forage to Firmicutes in silage at the phylum level. Lactobacillus remained the dominant genus in all silage. Linear discriminant analysis effect size (LEFSe) analysis identified Lactobacillus as relatively abundant in LP-treated silage and Weissella in LB-treated groups. The results of KEGG pathway analysis of the 16S rRNA gene of the silage microbial flora showed that the abundance of genes related to amino acid metabolism in the LP, LB, and LPB groups was lower than that in the CK group (p < 0.05). In conclusion, LAB application can improve the fermentation quality and nutritional value of WPSCS by regulating the succession of microbial communities and metabolic pathways during ensiling. Concurrently, the LB inoculant showed the potential to improve the aerobic stability of WPSCS.
“…In contrast, L. buchneri, a heterofermentative LAB, enhances silage's aerobic stability by converting LA into acetic acid (AA), thereby inhibiting filamentous fungi growth (Yin et al, 2023). da Costa et al (2022) reported that L. buchneri inoculation increased AA concentration and reduced yeast and ethanol content in sugarcane and peanut silages. Current research on whole-plant corn-soybean predominantly focuses on the intercropping model and the corn-soybean ratio during silage processing (Kizilsimsek et al, 2017;Ni et al, 2018;Zeng et al, 2020;Meng et al, 2022), and the above two mixed silage methods are also limited by nutrient loss and incomplete fermentation.…”
The mixture of whole-plant soybean and whole-plant corn silage (WPSCS) is nutrient balanced and is also a promising roughage for ruminants. However, few studies have investigated the changes in bacterial community succession in WPSCS inoculated with homofermentative and heterofermentative lactic acid bacteria (LAB) and whether WPSCS inoculated with LAB can improve fermentation quality by reducing nutrient losses. This study investigated the effect of Lactobacillus plantarum (L. plantarum) or Lactobacillus buchneri (L. buchneri) on the fermentation quality, aerobic stability, and bacterial community of WPSCS. A 40:60 ratio of whole-plant soybean corn was inoculated without (CK) or with L. plantarum (LP), L. buchneri (LB), and a mixture of LP and LB (LPB), and fermented for 14, 28, and 56 days, followed by 7 days of aerobic exposure. The 56-day silage results indicated that the dry matter content of the LP and LB groups reached 37.36 and 36.67%, respectively, which was much greater than that of the CK group (36.05%). The pH values of the LP, LB, and LPB groups were significantly lower than those of the CK group (p < 0.05). The ammoniacal nitrogen content of LB was significantly lower than that of the other three groups (p < 0.05), and the ammoniacal nitrogen content of LP and LPB was significantly lower than that of CK (p < 0.05). The acetic acid content and aerobic stability of the LB group were significantly greater than those of the CK, LP, and LPB groups (p < 0.05). High-throughput sequencing revealed a dominant bacteria shift from Proteobacteria in fresh forage to Firmicutes in silage at the phylum level. Lactobacillus remained the dominant genus in all silage. Linear discriminant analysis effect size (LEFSe) analysis identified Lactobacillus as relatively abundant in LP-treated silage and Weissella in LB-treated groups. The results of KEGG pathway analysis of the 16S rRNA gene of the silage microbial flora showed that the abundance of genes related to amino acid metabolism in the LP, LB, and LPB groups was lower than that in the CK group (p < 0.05). In conclusion, LAB application can improve the fermentation quality and nutritional value of WPSCS by regulating the succession of microbial communities and metabolic pathways during ensiling. Concurrently, the LB inoculant showed the potential to improve the aerobic stability of WPSCS.
The aim of this study was to evaluate silages produced with different mixtures of melon biomass and different levels of ground corn. The experiment was conducted under a completely randomized design in factorial scheme (3 × 4), with four replications. The first factor consieted of three mixtures of plant (branch and leaf) and fruit (melon; scraps) on natural matter (NM) as follows: 0, 100 and 1000 g kg-1 fruit. The second factor consisted of the use of different amounts of ground corn (0, 50, 100 and 200 g kg-1). Experimental silos with capacity for 5 kg and density of 500 kg m-3 were used. After 90 days, the silos were opened and the evaluations were carried out. The data were subjected to analysis of variance and the means were analyzed through the Tukey’s test (fermentative losses, chemical composition, aerobic stability), Scott-Knott test (microbiology and organic acids) and regression, and were compared with a significance level of p<0.05. The best dry matter results were obtained in silages with 0, 100 and 1000 g kg-1 fruit combined with 200 g kg-1 ground corn, which averaged 289.4, 290.4 and 264.1 g kg-1, respectively. Quadratic behavior was observed for effluent losses in silages with 100 g kg-1 fruit presenting a minimum value of 46.8 e and a maximum 56.2 kg t-1 NM. Regarding the mold population count, the highest values were obtained in silages with 100 g kg-1 fruit and 50 g kg-1 ground corn. Silages with 100 and 1000 g kg-1 fruit in melon biomass and 200 g kg-1 ground corn presented superior quality indicators, characterizing these biomass mixtures as the most suitable for silage making.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.