Mixed self-assembly of polydiacetylenes for highly specific and sensitive strip biosensors

Simple and highly sensitive detection of hepatotoxin microcystin-LR via colorimetric variation based on polydiacetylene vesicles

“…However, when an overdose of anti-MC-LR is absorbed onto the sensor surfaces, because there is insufficient room, the MC-LR may be prevented from approaching or the anti-MC-LR may not rotate to cause backbone change of PDA as a result of the steric effect. These responses are in accordance with research on the avidin-biotin interaction based on PDA [19]. This finding is highly significant for immunosensor fabrication dependent on three-dimensional conformations because, on one hand, it greatly reduces the unnecessary cost of expensive antibody; and on the other hand, it is effective in enhancing the sensitivity of biosensors.…”

“…The immunoassay based on PDA lipids has attracted much attention with its blue-to-red color and accompanying fluorescence changes [19,35]. In the present work, the use of EDC/NHS enabled mAbs of MC-LR (anti-MC-LR)-MC8C50 to covalently link to the carboxyl groups of PDA vesicles after photopolymerization, without substantially affecting the activity of the protein [21,34].…”

“…The optimal amount of anti-MC-LR coupled for recognition was investigated here to test a given amount of MC-LR at various anti-MC-LR concentrations. Due to the possible non-specific absorption of the PDA surfaces saturated with carboxyl [19], we used 150 g/mL BSA to block the non-specific spots and cleared off the excrescent by dialysis at 4 • C overnight. With a subsequent 30 min incubation at 37 • C following application of MC-LR onto the BSA blocked PDA-anti-MC-LR conjugates, the color of PDA vesicles varied.…”

“…In this paper, we report a novel detection strategy for hepatotoxin MC-LR based on polydiacetylene (PDA) vesicles that mimic biological interfaces upon which molecular events like immune reactions can be detected [17][18][19][20][21]. PDA is a unique conjugated polymer that shows a well-known stress-sensitive color change [22].…”

Biomimetic System Characterization: pH-Induced Chromatic Transition and Nanostructural Transformation of Polydiacetyle and Dimyristoylphosphatidylcholine Vesicles Under pH Variation Using Dynamic Light Scattering (DLS) Technique

“…This interest is due to the fact that PDA-based materials have different colorimetric characteristics, depending on some environmental conditions. Changes in their color, usually from blue to red, in response to some stimuli, such as temperature [1][2][3], pH [4,5], mechanical disturbances [6], solvents [7], and recognition of substances [8], have promoted the effective use of PDA vesicles in the development of colorimetric analysis [9][10][11], chips [12,13] and biosensors [14,15]. The correct mechanism to explain color transition from blue to red, due to different stimuli has not been discovered.…”