Enzyme modified electrodes were fabricated with poly(aniline-co-maminophenol). Electrochemical polymerization of aniline and m-aminophenol was performed to get the film of copolymer on the surface of gold electrode. Modified electrodes were fabricated by two methods, physical entrapment and covalent cross-linking. In one of the method, gold nanoparticles were loaded into the copolymer film and horseradish peroxidase (HRP) was immobilized into the Au nanoparticle loaded copolymer film through physical entrapment. In the other method, the amino and -OH groups in the copolymer are utilized to form covalent functionalization with HRP via glutaric dialdehyde as cross-linker/mediator. The conducting copolymer/enzyme modified electrodes prepared by physical entrapment/covalent functionalization of enzyme were tested for electrocatalytic activities towards sensing of H 2 O 2 . Amperometric results indicate that enzyme modified electrode via physical entrapment possesses better electrocatalytic performance over covalent functionalized enzyme electrode.
Interleukin-32 is a novel human cytokine implicated in several inflammatory and autoimmune diseases. Recombinant interleukin -32 can be produced and found to be useful in many fields. Until now, there has been no report on recombinant hIL-32 expressed in basidomycete fungus, Pleurotus eryngii. In this study, we examined whether the popular edible mushroom Pleurotus eryngii could support the expression of novel protein hIL-32. A binary vector pCAMBIA1304 containing the hIL-32 gene was constructed and introduced into Pleurotus eryngii via Agrobacterium tumefaciens-mediated transformation. The expression of hIL-32 was confirmed by PCR, Southern blot and western blot analysis. The recombinant hIL-32 reached a maximum expression level of 1.9 % of total soluble protein in transgenic mycelia. These results suggest that Pleurotus eryngii expression system can be effective for the production of rhIL-32 at an economically relevant level.
Heparin-immobilized Pluronic (F-68)/Polyvinylalcohol (PVA) composite microparticles were designed and characterized for the sustained drug delivery of ionic drug. Venlafaxine, antidepressant medication, was used as a model drug. For the efficient loading of ionic drug, heparin was immobilized into F-68/PVA composite microparticles. Differential scanning calorimetry (DSC) was used to understand the intra/intermolecular interactions in the heparin-immobilized F-68/PVA composite gels containing model drug. For the application as a sustained drug delivery system, the loading amount and release pattern of loaded drug were measured using high performance liquid chromatography (HPLC).
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