Mixed monolayer decorated SPR sensing surface for thrombin detection

Sadık, Demet Ataman; Boyaci, İsmail Hakkı; Mutlu, Mehmet

doi:10.1016/j.jpba.2019.112822

Cited by 8 publications

(3 citation statements)

References 26 publications

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“…The same research group has developed an immunosensor for thrombin by immobilizing anti-thrombin antibodies on the DSP/MCH layer. The linear range was 1.0–500.0 nM [ 25 , 26 ]. However, there are some disadvantages of metal coating with thiol-SAMs.…”

Section: Surface Modification In Spr Assays: Coating Strategiesmentioning

confidence: 99%

Strategies for Surface Design in Surface Plasmon Resonance (SPR) Sensing

2023

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Surface plasmon resonance (SPR) comprises several surface-sensitive techniques that enable the trace and ultra-trace detection of various analytes through affinity pairing. Although enabling label-free, sensitive detection and real-time monitoring, several issues remain to be addressed, such as poor stability, non-specific adsorption and the loss of operational activity of biomolecules. In this review, the progress over sensor modification, immobilization techniques and novel 2D nanomaterials, gold nanostructures and magnetic nanoparticles for signal amplification is discussed. The advantages and disadvantages of each design strategy will be provided together with some of the recent achievements.

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Section: Surface Modification In Spr Assays: Coating Strategiesmentioning

confidence: 99%

Strategies for Surface Design in Surface Plasmon Resonance (SPR) Sensing

2023

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“…SPR sensors have also been used in diverse healthcare biosensing applications, bioimaging paediatric burns [75] as well as in the study of mutations [76] and the effects of radiation exposure [77]. Recent studies have also shown the versatility of such devices in the study of pathogens [78], thermal injuries [79], carcinogens [80] and several clinically vital molecules [81][82][83].…”

Section: Surface Plasmon Resonance Sensors For Biomedical Diagnosticsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

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In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

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“…Thrombin is a serine protease, an enzyme that plays a key role in blood coagulation, and is relevant to a plethora of diseases such as chronic inflammatory diseases, vascular disease, coagulation abnormalities, cancer, and atherosclerosis. − Generally, excessive thrombin will cause venous thrombosis, and a low amount of thrombin will increase bleeding risks . The concentration of thrombin during coagulation varies from picomolar to micromolar levels, and thus, the limit of detection (LOD) and detection range need to cover the picomolar to micromolar range. , Moreover, recent studies have made progress in thrombin detection, including enzyme-linked sandwich assays, , strips, − optical sensors, − electrochemical platforms, − and piezoelectric methods . In general, enzyme-linked sandwich assays are the gold standard for thrombin detection but also experience laborious steps, low analysis speed, and false positives.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Quartz Crystal Microbalance Biosensor Based on Aptamer-Capped Gold Nanocages Loaded with Polyamidoamine for Thrombin Detection

Niyonshuti

et al. 2021

ACS Appl. Nano Mater.

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Thrombin is an important biomarker, and its detection is of great significance for diagnosis and prevention of related diseases. Conventional methods such as enzyme-linked immunosorbent assay are sensitive but require multiple steps. In this work, a label-free biosensor that only required one step of incubation was developed based on target-triggered release of the cargo molecules from gold nanocages, which achieved highly sensitive and specific detection of thrombin. The proposed biosensor consists of an array of gold nanocages loaded with molecules in their interiors and the DNA probes immobilized on their surface for hybridization with thrombin-specific aptamers to seal their pores. Upon interaction with thrombin, the surface aptamers were lifted off the gold nanocages, resulting in release of the cargo molecules. The loss of cargo molecules was detected by quartz crystal microbalance (QCM). The signal could be amplified by the choice of cargo molecules. The use of polyamidoamine as cargo molecules allowed us to achieve a label-free biosensor with a linear detection range of 0.0086−86 nM and a limit of detection of 7.7 pM. The biosensor was also tested with spiked human serum samples with a limit of detection of 1.2 nM. The detection could be carried out within 1.5 h with only one incubation step of 45 min. The specificity of the biosensor was confirmed by testing against bovine serum albumin and lysozyme at 1 μM.

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Mixed monolayer decorated SPR sensing surface for thrombin detection

Cited by 8 publications

References 26 publications

Strategies for Surface Design in Surface Plasmon Resonance (SPR) Sensing

Strategies for Surface Design in Surface Plasmon Resonance (SPR) Sensing

Recent Progress in Optical Sensors for Biomedical Diagnostics

Label-Free Quartz Crystal Microbalance Biosensor Based on Aptamer-Capped Gold Nanocages Loaded with Polyamidoamine for Thrombin Detection

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