2019
DOI: 10.1038/s41467-019-08417-5
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Mitotic chromosome binding predicts transcription factor properties in interphase

Abstract: Mammalian transcription factors (TFs) differ broadly in their nuclear mobility and sequence-specific/non-specific DNA binding. How these properties affect their ability to occupy specific genomic sites and modify the epigenetic landscape is unclear. The association of TFs with mitotic chromosomes observed by fluorescence microscopy is largely mediated by non-specific DNA interactions and differs broadly between TFs. Here we combine quantitative measurements of mitotic chromosome binding (MCB) of 501 TFs, TF mo… Show more

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Cited by 89 publications
(113 citation statements)
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“…Cells were cultured and prepared as described in 25 . Cells with stable integration of Halo-CDX2 under doxycycline-induced expression control (kind gift from the lab of David Suter, EFPL, Lausanne, Switzerland) were seeded one day before the measurement on a Delta-T glass bottom dish (Bioptechs, Pennsylvania, USA).…”
Section: Cell Culture and Preparationmentioning
confidence: 99%
“…Cells were cultured and prepared as described in 25 . Cells with stable integration of Halo-CDX2 under doxycycline-induced expression control (kind gift from the lab of David Suter, EFPL, Lausanne, Switzerland) were seeded one day before the measurement on a Delta-T glass bottom dish (Bioptechs, Pennsylvania, USA).…”
Section: Cell Culture and Preparationmentioning
confidence: 99%
“…We then aimed to identify the molecular mechanisms by which small and transient endogenous fluctuations of SOX2 and OCT4 result in major biases in differentiation potential. As SOX2 and OCT4 were shown to regulate chromatin accessibility (King & Klose, 2017;Raccaud et al, 2019), we reasoned that small changes in their expression level could prime cells for different fates by altering the chromatin accessibility landscape. We thus performed ATAC-seq in G1-sorted SHOH, SHOL, SLOH and SLOL cells.…”
Section: Oct4-high Cells Open Differentiation Enhancersmentioning
confidence: 99%
“…Proliferating cells show a global downregulation of transcription during mitosis. This results from the 2 combination of three main processes: 1) nuclear envelope breakdown leading to an increase of the volume 3 that transcription factors (TFs) and the RNA polymerases II (RNAPII) can explore and therefore 4 a decrease of their local concentration around gene promoters; 2) major reorganization of chromatin 5 architecture characterized by chromosome condensation, repositioning of nucleosomes in some regulatory 6 regions, loss of long-range interaction between enhancers and promoters and disassembling of topological 7 associated domains (TADs); and, 3) TF-DNA binding inactivation through postranscriptionally regulated 8 phosphorylation. As a consequence, most TFs and the RNAPII are evicted from mitotic chromosomes 9 and RNA synthesis is drastically reduced [1].…”
Section: Introductionmentioning
confidence: 99%