Mitogen- and antigen-responsive milk lymphocytes

Smith, Janet W.; Schultz, Ronald D.

doi:10.1016/0008-8749(77)90285-4

Cited by 59 publications

(16 citation statements)

References 12 publications

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“…This hyporesponsiveness has been observed previously (4,5). Milk cells from two coeliac subjects showed significantly increased proliferation to Con-A and to gluten, but not to soya bean antigen.…”

Section: Discussionsupporting

confidence: 86%

“…Funetionally, milk lymphoeytes of activation of milk cells from coeliae subjeets are hyporesponsive to mitogens and most antiis unknown. In a companion study, the present gens, but sometimes show reciprocal stimulaauthors have already demonstrated that sensition to certain antigens for whieh the peripheral tized CD3+ and CD4+ T lymphocytes in the systemie response is poor (4,5).…”

Section: Introductionmentioning

confidence: 57%

See 1 more Smart Citation

Phenotype and activation of milk‐derived and peripheral blood lymphocytes from normal and coeliac subjects

Ba²,

Ia³

et al. 1991

Immunology & Cell Biology

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Summary The phenotype of milk-derived and peripheral blood lymphoeytes from normal and coeliac subjects was assessed for CD3. aP-TcR (T cell receptor). yS-TcR. CD4. CD8. HML-I (human mucosal lymphocyte) determinants, and activation was measured by interleukin-2 receptor {1L-2R) expression. Milk cells from normal and coeliac subjects were analysed by manual immunofluoreseence and milk and blood cells from normal subjects were analysed by flow cytometry. Milk eells from two eoeliac subjects were tested for proliferation to gluten antigen. The CD4:CD8 ratio of milk lymphoeytes from both normal and eoeliac subjects was similar (0.78-1.1). but lower than that present in blood (1.5-2.1). The IL-2R expression of milk lymphocytes from both coeliac and normal subjeets was increased by 3-6 times eompared with peripheral blood eells. For example. IL-2R was present on 27.3% of milk CD3+ lymphocytes and on 8.0% of blood CD3+ lymphocytes from normal subjects. Y5-and HML-I+ T cells were inereased 4.2-fold and 12-fold respeetively compared with blood lymphocytes. Milk cells from coeliac subjects showed specific proliferation to gluten but not to soya bean antigen. We conclude that milk cells have a 'mucosal" phenotype. with increased Y5-TCR. CD8+ and HML-I+ T cells, and have an inereased proportion of aetivated eells. Milk eells from coeliae subjects have no 'toxic' phenotype. but show functional reactivity by speeific proliferation to gluten antigen.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 57%

Phenotype and activation of milk‐derived and peripheral blood lymphocytes from normal and coeliac subjects

Ba²,

Ia³

et al. 1991

Immunology & Cell Biology

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“…Identification of lymphocyte subpopulations was not attempted, but it is likely that they are similar to those in human colostrum [Diaz-Jouanen and Williams, 1974], bovine [Smith and Schultz, 1977;Concha et al, 1978] and ovine [Lee and Outteridge, 1981] milk, where both T and B cells are present. However, further work is necessary to elucidate this point.…”

Section: Discussionmentioning

confidence: 99%

Cytology of Milk in Guinea Pigs

Nordin¹,

Lee²

1982

Cells Tissues Organs

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Identification, total and differential counts of cell populations in guinea pig colostrum, milk and involution secretion were carried out. The cellular concentration in colostrum was high, but there was a dramatic decline in milk collected at the peak of lactation. Advancement of mammary gland involution was associated with a dramatic increase in cellular concentration. The neutrophil was the predominant cell type in colostrum, milk and early involution secretion. Macrophages were present at all stages of lactation and became the predominant cell as involution advanced. The proportion of lymphocytes was low and remained constant throughout the various stages of lactation. Small numbers of eosinophils and epithelial cells were present in most of the samples examined.

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“…The in vivo functions of the stimulatory activ ities for cytokine production observed in hu man milk are unknown. These can act on the mononuclear cells present in the milk [26][27][28] which have been shown to have the capacity to respond to immunogenic stimulation [29][30] and to produce cytokines such as IL-1 [31][32][33], IL-2 [31] and interferon-y [33], Alter natively, the stimulatory activities for cyto kine production may act on the oropharyngeal and intestinal-associated lymphoid tissues. From these sites activated lymphocytes and monocytes can influence other distant lym phoid and nonlymphoid tissues thus inducing immunostimulation.…”

Section: Discussionmentioning

confidence: 99%

Human Colostrum Stimulates Cytokine Production

Bessler¹,

Straussberg

Hart

et al. 1996

Neonatology

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The effect of human colostrum on the production of IL-1, IL-3 and IL-6 by peripheral blood mononuclear cells (PBMCs) has been investigated. The aqueous phase of human colostrum significantly stimulated the production of these three cytokines. These findings show the importance of breast feeding not only as a well-balanced nutrient supply but also as a source for growth-promoting factors. It is suggested that the enhanced secretion of IL-1, IL-3 and IL-6 induced by human colostrum may compensate for the lower capacity of neonatal PBMCs to produce these cytokines. It is also possible that, by stimulating the secretion of these cytokines, breast feeding may provide an additional mechanism for the regulation of the neonatal immune system and hematopoiesis.

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Mitogen- and antigen-responsive milk lymphocytes

Cited by 59 publications

References 12 publications

Phenotype and activation of milk‐derived and peripheral blood lymphocytes from normal and coeliac subjects

Phenotype and activation of milk‐derived and peripheral blood lymphocytes from normal and coeliac subjects

Cytology of Milk in Guinea Pigs

Human Colostrum Stimulates Cytokine Production

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