Mitochondrial RNA from cultured animal cells. Distinctive high-molecular-weight and 4 s species

Dubin, Donald T.; Montenecourt, Bland S.

doi:10.1016/0022-2836(70)90161-0

Cited by 86 publications

(31 citation statements)

References 43 publications

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“…The only striking metabolic alteration observed was the cessation of cytoplasmic rRNA synthesis (cf . 2,11,12) . In addition, as was found with BHK (2) and HeLa cells (13,14), standard preparative methods did not yield L-cell mitochondria completely free of cytoplasmic ribosomes.…”

Section: Preliminary Studiesmentioning

confidence: 99%

“…Hence, in the experiments described below, mitochondria were prepared only from cultures labeled in the presence of actinomycin D (0 .1 µg/ml, 22-24 hr) . When uridine-14C-labeled mitochondrial RNA prepared from such L-cell cultures was fractionated by density gradient centrifugation under conditions designed to display the cytoplasmic ribosomal RNA components, a "I 7s" peak resembling the 2 4 6 unresolved high molecular weight RNA peak of BHK cells was observed (1,2) .…”

Section: Preliminary Studiesmentioning

confidence: 99%

“…1, the 14C and 32 P patterns were quite similar ; under these centrifugal conditions the discrete high molecular weight mitochondrial RNA from both and 32P (10 µCi/1 .2 µmoles/ml), respectively, in the presence of 0 .1 µg/ml of actinomycin D . Mitochondria were purified in parallel by differential and isopycnic gradient centrifugation (2) . The mitochondrial bands were pooled, unlabeled L-cell cytoplasmic RNA was added, and the RNA was extracted with 2% sodium dodecyl sulfate (SDS) and purified by shaking with phenol at room temperature as previously described (15) .…”

Section: Preliminary Studiesmentioning

confidence: 99%

“…Cells were fractionated in hypotonic buffer, and mitochondria were purified by differential and isopycnic gradient centrifugation (2) . The procedures for purification and analysis of RNA are described in the legends to Figs .…”

Section: Methodsmentioning

confidence: 99%

“…We have recently described distinctive high molecular weight RNA associated with the mitochondria of cultured hamster (BHK-21) cells (1,2) . This RNA sedimented slightly slower than cytoplasmic 18s RNA and differed from the latter in that it had an unusually low GC content, was very poorly methylated, and was synthesized in the presence of a level of actinomycin D (0 .1 µg/ml) that preferentially suppresses cytoplasmic ribosomal RNA (rRNA) synthesis .…”

Section: Introductionmentioning

confidence: 99%

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Mitochondrial Rna From Cultured Animal Cells

Montenecourt

Langsam

Dubin

1970

The Journal of Cell Biology

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Discrete RNA fractions sedimenting slightly slower than 18s ribosomal RNA have been found in mitochondrial preparations from both hamster (BHK-21) and mouse (L-929) cells . This RNA could be separated into two components, present in approximately equimolar amounts, by prolonged zonal centrifugation or acrylamide gel electrophoresis . The hamster components had sedimentation constants averaging 16 .8 and 13 .4, and molecular weights (estimated by gel electrophoresis) averaging 0.74 and 0.42 X 106 daltons . Mixed labeling experiments showed that the mouse components sedimented and electrophoresed 3-6% more slowly than the corresponding hamster components . The RNA from both cell lines resembled mitochondrial ribosomal RNA from yeast and Neurospora in being GC poor, and in addition the larger and smaller components resembled each other in base composition . These results, taken with those of other recent studies, are compatible with the idea that our high molecular weight mitochondrial RNA is ribosomal ; such RNA would then constitute a uniquely small size-class of ribosomal RNA .

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Section: Preliminary Studiesmentioning

confidence: 99%

Section: Preliminary Studiesmentioning

confidence: 99%

Section: Preliminary Studiesmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mitochondrial Rna From Cultured Animal Cells

Montenecourt

Langsam

Dubin

1970

The Journal of Cell Biology

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The elucidation of the human mitochondrial genome: A historical perspective

Attardi

1986

BioEssays

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SummaryThe progressive unraveling over the past fifteen years of the structure and function of the human mitochondrial genome, taken as a prototype of all vertebrate mitochondrial genomes, has been marked by a series of startling discoveries. The history of these developments is one in which prediction ojien turned out to be wrong, and in which solidly established dogmas were violated. The unique features of this genome have forced a revision of our ideas about the universality of the genetic code and of the decoding mechanism, the minimalstructural requirements for rRNA, tRNA and mRNA function and the mode and control of gene expression.

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A compendium of human mitochondrial gene expression machinery with links to disease

Shutt

Shadel

2010

Environ and Mol Mutagen

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Mammalian mitochondrial DNA encodes thirty-seven essential genes required for ATP production via oxidative phosphorylation, instability or misregulation of which is associated with human diseases and aging. Other than the mtDNA-encoded RNA species (thirteen mRNAs, 12S and 16S rRNAs, and twenty-two tRNAs), the many remaining factors needed for mitochondrial gene expression (i.e. transcription, RNA processing/modification and translation), including a dedicated set of mitochondrial ribosomal proteins, are products of nuclear genes that are imported into the mitochondrial matrix. Herein, we inventory the human mitochondrial gene expression machinery, and while doing so highlight specific associations of these regulatory factors with human disease. Major new breakthroughs have been made recently in this burgeoning area that set the stage for exciting future studies on the key outstanding issue of how mitochondrial gene expression is regulated differentially in vivo. This should promote a greater understanding of why mtDNA mutations and dysfunction cause the complex and tissue-specific pathology characteristic of mitochondrial disease states and how mitochondrial dysfunction contributes to more common human pathology and aging.

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Mitochondrial RNA from cultured animal cells. Distinctive high-molecular-weight and 4 s species

Cited by 86 publications

References 43 publications

Mitochondrial Rna From Cultured Animal Cells

Mitochondrial Rna From Cultured Animal Cells

The elucidation of the human mitochondrial genome: A historical perspective

A compendium of human mitochondrial gene expression machinery with links to disease

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