Mitochondrial Rna From Cultured Animal Cells

Montenecourt, Bland S.; Langsam, Margaret E.; Dubin, Donald T.

doi:10.1083/jcb.46.2.245

Cited by 20 publications

(5 citation statements)

References 22 publications

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“…The molecular weights are, therefore, probably close to the true values. The values are similar to those found for mammalian mitochondrial rRNA species by Robberson et al (1971), Montenecourt et al (1970) and Groot et al (1970). More reliable values must await a chemical or physical measurement that is independent of conformation.…”

Section: Discussionsupporting

confidence: 78%

“…Mitochondrial rRNA of the rodents, rat and mouse, had the same SE value but differed from those of human and hamster. Montenecourt et al (1970) previously reported that mouse L-cell mitochondrial rRNA has lower S and higher SE values than hamster BHK-21 cell mitochondrial rRNA. This can be most easily explained by a more unfolded conformation for L-cell mitochondrial rRNA.…”

Section: Discussionmentioning

confidence: 90%

“…Mitochondria from mammalian cells contain ribosomal RNA different from that of the cytoplasm (Vesco & Penman, 1969;Bartoov et al, 1970;). Montenecourt et al (1970) found that mitochondrial rRNA species from hamster and mouse differ in electrophoretic mobilities and sedimentation coefficients. The significance of this is unclear because the physical properties of mitochondrial rRNA from mammalian as well as other species is highly dependent on the ionic composition of the solvent and the temperature (Groot et al, 1970;Forrester et al, 1970;Edelman et al, 1971;Grivell et al, 1971).…”

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confidence: 99%

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Comparison of mammalian mitochondrial ribosomal ribonucleic acid from different species

Mitra

Bartoov

Monahan

et al. 1972

Biochemical Journal

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Mitochondrial ribosomal RNA species from mouse L cells, rat liver, rat hepatoma, hamster BHK-21 cells and human KB cells were examined by electrophoresis on polyacrylamide-agarose gels and sedimentation in sucrose density gradients. The S(E) (electrophoretic mobility) and S values of mitochondrial rRNA of all species were highly dependent on temperature and ionic strength of the medium; the S(E) values increased and the S values decreased with an increase in temperature at a low ionic strength. At an ionic strength of 0.3 at 23-25 degrees C or an ionic strength of 0.01 at 3-4 degrees C the S and S(E) values were almost the same being about 16.2-18.0 and 12.3-13.6 for human and mouse mitochondrial rRNA. The molecular weights under these conditions were calculated to be 3.8x10(5)-4.3x10(5) and 5.9x10(5)-6.8x10(5), depending on the technique used. At 25 degrees C in buffers of low ionic strength mouse mitochondrial rRNA species had a lower electrophoretic mobility than those of human and hamster. Under these conditions the smaller mitochondrial rRNA species of hamster had a lower electrophoretic mobility than that of human but the larger component had an identical mobility. Mouse and rat mitochondrial rRNA species had identical electrophoretic mobilities. Complex differences between human and mouse mitochondrial rRNA species were observed on sedimentation in sucrose density gradients under various conditions of temperature and ionic strength. Mouse L-cell mitochondrial rRNA was eluted after cytoplasmic rRNA on a column of methylated albumin-kieselguhr.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 90%

mentioning

confidence: 99%

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Comparison of mammalian mitochondrial ribosomal ribonucleic acid from different species

Mitra

Bartoov

Monahan

et al. 1972

Biochemical Journal

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“…The temperature of electrophoresis can affect the relative mobilities of RNA molecules, as was demonstrated with mitochondrial rRNA species (Groot et al, 1970;Montenecourt et al, 1970). In all our experiments electrophoresis was carried out at 20-25°C, so that the mobility differences we observed cannot be due to large variations in the temperature of electrophoresis.…”

Section: Effects Ofchanges In Salt Concentration and Ofheat On Apparementioning

confidence: 73%

Amphibian ribosomal ribonucleic acids

Rogers

Klein

1972

Biochemical Journal

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1. The larger rRNA molecules of different species of amphibia do not all have the same mobility on polyacrylamide gels, and the difference appears to be one between the Anuran and Urodele amphibia. 2. Alteration of conditions of electrophoresis so as to distinguish between differences of conformation and molecular weight does not affect the results. 3. The mobility on gel electrophoresis of the rRNA precursor in two species of amphibia is similar, although the intermediate processing products differ. 4. It is suggested that the mobility differences observed represent differences of molecular weight, and are an example of the variability of the products of transcription and processing of rRNA genes in two amphibian genera.

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“…Reports have appeared on large molecular weight RNAs associated with the mitochondria of higher organisms. These include HeLa cells [ 1 ] , BHK-2 1 cells [2] , mouse L-cells [3,4] , rat liver [4, S] and Xenopus ovary [6] . This RNA consists of two predominant species found to be 17 S and 13 S by sedimentation [2, 31 .…”

Section: Introductionmentioning

confidence: 99%