The mitochondrial ribosome and ribosomal RNA of the chick

Rabbitts, Terence H.; Work, T. S.

doi:10.1016/0014-5793(71)80620-8

Cited by 16 publications

(3 citation statements)

References 19 publications

(5 reference statements)

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“…0 , 30-min incubation; 0-0, 60-min incubation in vitro reported earlier [15] and shows that the same 5 5 3 particle which is detected as the functional unit of protein synthesis in vitro also exists and has an identical function in the intact animal. As shown by Rabbits and Work [23], this particle contains RNA molecules with sedimentation values of 18 S and 12 S (by electrophoresis), which identify it as the mitoribosome. As a final check on the identity and function of the 55-5 particle from mitochondria we followeed labelling with [SH]leucine in a culture of BHK-21 cells.…”

Section: Identification Of a 55-s Mitoribosome Active In Protein Syntmentioning

confidence: 81%

Proteins Coded by Mitochondrial DNA of Mammalian Cells

Coote¹,

Work²

1971

Eur J Biochem

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Improved conditions are described for the maintenance of protein synthesis by isolated liver mitochondria. The products of synthesis in vitro are first attached to a 55-S mitoribosome (mitochondrial-ribosome) but move off the mitoribosome as completed peptide chains in less than 15 min a t 30 "C. Over a 1-h period of incubation there is no change in the spectrum of molecular weights of the peptides being synthesized. The protein synthesized by isolated hamsterliver mitochondria were compared with those synthesized by whole cells under conditions where cytoplasmic protein synthesis had been blocked by emetine. The proteins synthesized by whole cell mitochondria were indistinguishable from those synthesized by isolated mitochondria. By the combined use of emetine, chloramphenicol and ethidium bromide as specific inhibitors of cytoribosomal and mitoribosomal protein synthesis it was possible to calculate that 7 of the total mitochondrial protein was coded by the mitochondrial genome. A phosphate buffer a t p H 11.5 solubilized about goo/, of the total protein of the mitochondrion but left over goo/, of the protein coded by the mitochondrial genome as an insoluble residue. This fraction on polyacrylamide in the presence of sodium dodecylsulphate and mercaptoethanol was shown to contain a t least 20 distinct proteins. At least 10 of these were coded by the mitochondrial genome and their molecular weights ranged from 14000 to 50000. These proteins together with the mitochondrial tRNA and the mitoribosomal RNA account for most of the information available in the mitochondrial genome.Investigations in several laboratories (see review, Ashwell and Work [l]) have shown that mitochondria are semi-autonomous bodies which contain informational DNA, mitoribosomes (mitochondrial ribosomes) and a mechanism for protein synthesis which is similar to but quite distinct from that present in cytoplasm of the same cells.It has been shown that the 16-S and 12-S RNA of mitoribosomes is coded by mitochondrial DNA, as are an uncertain number of transfer RNA species[a]. The molecular weights of various types of mammalian mitochondrial DNA molecules are all about 10000000, and the DNA is a double-stranded circular molecule, only one strand of which seems to be informational [3]. Hybridization experiments have indicated that the genes for mitoribosomal RNA are not reiterated on the mitoribosomal DNA [3] so that some of the mitochondrial genes are certainly available to code for messenger RNA. This messenger RNA appears to code for proteins which form a n essential part of the structure of the inner mitochondrial membrane and in their absence the enzymes of electron transport, which are coded by nuclear genes, accumulate in the cytoplasm and cannot be integrated into their active configuration in the cristae membrane. It thus appears that the mitochondrial Enzyme. Lactate dehydrogenase (EC 1.1.1.27); pyruvate kinase (CE 2.7.1.40); lyoszyme (EC 3.2.1.17).genes code for organizer proteins essential to the life of the cell [l].It has been our p...

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Section: Identification Of a 55-s Mitoribosome Active In Protein Syntmentioning

confidence: 81%

Proteins Coded by Mitochondrial DNA of Mammalian Cells

Coote¹,

Work²

1971

Eur J Biochem

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“…Values within the range 54S to 61S have been obtained for these particles from rat (O'Brien and Kalf, 1967), HeLa cells (Perlman and Penman, 1970), rabbit, pig, cow (O'Brien. 1971), hamster (Coote et al , 1971), chicken (Rabbitts and Work, 1971),…”

Section: Mitochondrial Ribosomes Of Animalsmentioning

confidence: 99%

Proteins of the mammalian mitochondrial ribosome / by David Earl Matthews.

Matthews¹

1976

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“…and 40S for the ribosomal subunits (Swanson and Dawid, 1970;Rabbitts and Work, 1971;Aaij et al, 1972;DeVries and Van der Koogh-Schuuring, 1973;Sacchi et al, 1973;O'Brien et al, 1974;O'Brien and Kalf, 1976b). In contrast, the sedimentation value for animal cytoplasmic ribosomes is 80S with 40S and 60S subunits (O'Brien et al, 1974).…”

Section: Mammalian Mitochondrial Mrnas and Protein-coding Genesmentioning

confidence: 99%

Studies of the HeLa mitochondrial translation system

Girard

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The mitochondrial ribosome and ribosomal RNA of the chick

Cited by 16 publications

References 19 publications

Proteins Coded by Mitochondrial DNA of Mammalian Cells

Proteins Coded by Mitochondrial DNA of Mammalian Cells

Proteins of the mammalian mitochondrial ribosome / by David Earl Matthews.

Studies of the HeLa mitochondrial translation system

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