Mitochondrial adenosinetriphosphatase system. III. Isolation from the oligomycin-sensitive adenosinetriphosphatase complex of the factors which bind F1 and determine oligomycin sensitivity of bound F1

Subunit equivalence in Escherichia coli and bovine heart mitochondrial F1F0 ATPases

“…Similarly, e-subunits are probably non-equivalent. The available sequence information suggests that the counterpart of the E.coli 8-subunit is the bovine mitochondrial oscp, a basic protein known to play an important part in binding FI to F0 [8]. The bacterial 8-subunit has a similar role [12].…”

“…Equivalence of subunits with the same names (a-c) in mitochondria and E.coli is often ~, assumed despite considerable size differences between the 8-subunits and between the c-subunits ( fig.l). The bacterial F0 is made of 3 subunits a, b ~ and c; mitochondrial F0 appears to be more complex: extra subunits have been detected in this sector, the best characterised being the oligomycin ~ sensitivity conferral protein (oscp) [6][7][8][9] and factor 6 (F6) [10]. The oscp is a basic, water-soluble protein of -18 000 Mr [5].…”

Mitochondrial Genes and Translation Products

“…The yeast complex consists of at least ten different protein subunits (51) none of whose functions can be clearly stated at present. The oligomycin-sensitive ATPase complexes of bovine heart and yeast mitochondria have been dissected into three fractions that can spontaneously reassociate to form the native enzyme (52,53). The three reconstitutively active fractions are Fl (54), OSCP (55), and a set of hydrophobic proteins referred to as the membrane factor (52).…”

Cross‐reconstitution of isolated F1‐ATPase from potato tuber mitochondria with F1‐depleted beef heart and yeast submitochondrial particles

“…Fr-depleted submitochondrial particles from yeast were prepared by treatment of the particles with 3.5 M NaBr, as described for beef heart particles [21].…”