Cross‐reconstitution of isolated F₁‐ATPase from potato tuber mitochondria with F₁‐depleted beef heart and yeast submitochondrial particles

Abstract

Cross-reconstitution of isolated potato mitochondrial Fr-ATPase with Fr-depleted beef heart and yeast submitochondrial particles is reported. Potato Fr binds to the heterologous membrane and confers oligomycin sensitivity on the ATPase activity of the reconstituted system. Binding of F1 is promoted by the presence of Mg2+ with the maximal stimulatory effect at 20 mM. Mgz+ increase the sensitivity to oligomycin of the reconstituted system consisting of potato F1 and yeast membranes, however, they do not influence oligomytin sensitivity of potato Fr and heef heart membranes.

Hybrid Fo Complexes of the ATP Synthases of Spinach Chloroplasts and Escherichia coli

“…studied in vitro (for example, see Bar-Zvi et al, 1985;Gromet-Elhanan et al, 1985;Richter et al, 1986;Kauffer et al, 1987;Steffens et al, 1987;Norling et al, 1987;Horak et al, 1988;Engelbrecht et al, 1989;Laubinger et al, 1990) and in vivo (for example, see Hawthorne and Brusilow, 1986;Scarpetta et al, 1991;Munn et al, 1991;Kaim et al, 1992;Kaim and Dimroth, 1993;Lill et al, 1993). However, hybrid F, complexes have only been studied for the chloroplast and the E. coli system (Schmidt et al, 1990;Schmidt, 1992;Burkovski et al, 1990).…”