miRNAs: Nanomachines That Micromanage the Pathophysiology of Diabetes Mellitus

Sharma, Shilpy; Mathew, Abraham B; Chugh, Jeetender

doi:10.1016/bs.acc.2017.06.003

Cited by 13 publications

(5 citation statements)

References 262 publications

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“…Abnormal microRNAs (miRNAs) have been identified in various human diseases with an important functional role in disease pathogenesis [7]. Numerous miRNAs have been demonstrated to be involved in the progression of DM, as well as various complications of DM, including DR [8,9]. For example, miR-21 has been determined as a novel therapeutic target of DR by regulating retinal neovascularization and inflammation [10].…”

Section: Introductionmentioning

confidence: 99%

Dysregulation of miR-210 is involved in the development of diabetic retinopathy and serves a regulatory role in retinal vascular endothelial cell proliferation

Yin¹,

Lin²,

Sun³

et al. 2020

Eur J Med Res

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Background: Diabetic retinopathy is a common complication of diabetes mellitus (DM). The purpose of this study was to investigate the expression and clinical significance of miR-210 in DR patients and explore the regulatory effect of miR-210 on vascular endothelial cell function under high-glucose condition. Methods: Quantitative real-time PCR was used to estimate miR-210 expression. A receiver operating characteristics curve (ROC) was plotted to evaluate the diagnostic value of miR-210. Human umbilical vein endothelial cells (HUVECs) were used and treated with high glucose (30 mM), and the cell proliferation was assessed by MTT assay.Results: Serum expression of miR-210 was upregulated in DR patients compared with DM without DR patients and healthy controls. The expression of miR-210 in proliferative DR (PDR) patients was higher than non-proliferative DR (NPDR) patients. The increased serum miR-210 could be used to distinguish DR cases from healthy individuals and also simple DM patients, and can screen PDR cases from NPDR cases. The overexpression of miR-210 promoted HUVEC proliferation, while the knockdown of miR-210 resulted in the opposite effect under a high-glucose condition. Conclusion:The data of this study demonstrated that serum increased miR-210 serves as a diagnostic biomarker in DR patients and may have the ability to predict DR development and severity. The regulatory effect of miR-210 on vascular endothelial cell proliferation under high-glucose condition, indicating its therapeutic potential in the treatment of diabetic vascular diseases.

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Section: Introductionmentioning

confidence: 99%

Dysregulation of miR-210 is involved in the development of diabetic retinopathy and serves a regulatory role in retinal vascular endothelial cell proliferation

Yin¹,

Lin²,

Sun³

et al. 2020

Eur J Med Res

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show abstract

“…miRNAs are endogenous noncoding RNA generated by splicing of pre-miRNAs with a hairpin structure. 14 In recent years, study has found that miRNAs were involved in many important processes of diabetes through the regulation of cytokine expression. 15 For example, previous literature reported that miRNA-145-3p (miR-145-3p) was highly expressed in the human peripheral blood mononuclear cells (PBMCs) of patients with diabetes and attended to the development of neuropathy of diabetes.…”

Section: Introductionmentioning

confidence: 99%

Retracted: Circular RNA ACR relieves high glucose‐aroused RSC96 cell apoptosis and autophagy via declining microRNA‐145‐3p

2019

J of Cellular Biochemistry

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Retraction: ‘Circular RNA ACR relieves high glucose‐aroused RSC96 cell apoptosis and autophagy via declining microRNA‐145‐3p’, by Ying Liu, Xiaoqing Chen, Jingjing Yao, Jing Kang, J Cell Biochem. The above article, published online on 30 December 2019 in Wiley Online Library (https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.29568), has been retracted by agreement between the the journal's Editor in Chief, Prof. Dr. Christian Behl, and Wiley Periodicals LLC. The retraction has been agreed following an investigation based on allegations raised by a third party. Several flaws and inconsistencies between results presented and experimental methods described were found, the editors consider the conclusions of this article to be invalid. The authors collaborated in the investigation initially, but were not available for a final confirmation of the retraction.

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“…In addition, studies have shown that miR-378, miR-126-3p and miR-223 in blood are indicators of disease staging and prognosis in elderly patients with T2D (22) . In conclusion, serum miRNAs are noninvasive and ideal indicators for the diagnosis and prognosis of T2D (23) . However, no miRNA has been recognized as a speci c diagnostic marker for T2D.…”

Section: Discussionmentioning

confidence: 91%

“…However, recent studies have con rmed that epigenetic mechanisms play an important role in the pathophysiological process of T2D (3) . microRNA (miR) is a highly conserved non-coding small RNA widely distributed in eukaryotic cells, [18][19][20][21][22][23][24][25] nucleotides long, being capable of affecting the biological functions of other genes by binding to the 3'UTR end of its target gene (4,5) . More and more evidences (6)(7)(8) show that miRNAs are involved in the regulation of glucose metabolism, insulin synthesis and secretion, which are the core links in the development of T2D.…”

Section: Introductionmentioning

confidence: 99%

miR-720 Regulates Insulin Secretion by Targeting Rab35

Wang

Feng

et al. 2020

Preprint

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Background: miRNAs pose a good prospect in the diagnosis and treatment of type 2 diabetes (T2D). This study aimed to investigate whether miR-720 targets Rab35 to regulate insulin secretion in MIN6 cells and its molecular mechanism, and the clinical value of miR-720 as a specific biomarker of T2D. Methods: Fifty-five samples of new diagnosis T2D patients and normal control were collected. Levels of miR-720, fasting blood glucose, insulin and other indicators of glucose and lipid metabolism were determined. We increased and decreased miR-720 expression using miR-720 mimic and inhibitor to identify the effect of miR-720 on insulin secretion in MIN6 cells, respectively. Then we used miR-720 mimic, miR-720 inhibitor and dual luciferase reporter gene assays to prove miR-720 regulate insulin secretion by targeting Rab35 in MIN6 cells. In addition, we overexpressed and silenced of Rab35 gene to detect the expression of PI3K, Akt and mTOR in MIN6 cells by RT-PCR and western blot. Results: Circulating miR-720 was significantly higher in T2D group than control group, and miR-270 was positive correlated with fasting blood glucose, while negatively correlated with insulin. Overexpression of miR-720 inhibited insulin secretion and miR-720 downregulation promoted insulin secretion. miR-720 regulated insulin secretion by targeting Rab35 in MIN6 cells. Compared with the control group, the expression of PI3K, Akt and mTOR was significantly decreased by overexpression of Rab35 gene, while silencing Rab35 gene could induce the expression of PI3K, Akt and mTOR. Conclusions: We conclude that miR-720 may as a potential biomarker for the diagnosis of T2D. Increase of miR-720 reduced Rab35 expression then activate PI3K/Akt/mTOR signal pathway, thus inhibiting insulin secretion.

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miRNAs: Nanomachines That Micromanage the Pathophysiology of Diabetes Mellitus

Cited by 13 publications

References 262 publications

Dysregulation of miR-210 is involved in the development of diabetic retinopathy and serves a regulatory role in retinal vascular endothelial cell proliferation

Dysregulation of miR-210 is involved in the development of diabetic retinopathy and serves a regulatory role in retinal vascular endothelial cell proliferation

Retracted: Circular RNA ACR relieves high glucose‐aroused RSC96 cell apoptosis and autophagy via declining microRNA‐145‐3p

miR-720 Regulates Insulin Secretion by Targeting Rab35

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