Dysregulation of miR-210 is involved in the development of diabetic retinopathy and serves a regulatory role in retinal vascular endothelial cell proliferation

Abstract

Background: Diabetic retinopathy is a common complication of diabetes mellitus (DM). The purpose of this study was to investigate the expression and clinical significance of miR-210 in DR patients and explore the regulatory effect of miR-210 on vascular endothelial cell function under high-glucose condition. Methods: Quantitative real-time PCR was used to estimate miR-210 expression. A receiver operating characteristics curve (ROC) was plotted to evaluate the diagnostic value of miR-210. Human umbilical vein endothelial cells (HUVECs) were used and treated with high glucose (30 mM), and the cell proliferation was assessed by MTT assay.Results: Serum expression of miR-210 was upregulated in DR patients compared with DM without DR patients and healthy controls. The expression of miR-210 in proliferative DR (PDR) patients was higher than non-proliferative DR (NPDR) patients. The increased serum miR-210 could be used to distinguish DR cases from healthy individuals and also simple DM patients, and can screen PDR cases from NPDR cases. The overexpression of miR-210 promoted HUVEC proliferation, while the knockdown of miR-210 resulted in the opposite effect under a high-glucose condition. Conclusion:The data of this study demonstrated that serum increased miR-210 serves as a diagnostic biomarker in DR patients and may have the ability to predict DR development and severity. The regulatory effect of miR-210 on vascular endothelial cell proliferation under high-glucose condition, indicating its therapeutic potential in the treatment of diabetic vascular diseases.

To the Future: The Role of Exosome-Derived microRNAs as Markers, Mediators, and Therapies for Endothelial Dysfunction in Type 2 Diabetes Mellitus

“…Conversely, miR-495-3p was reportedly lower in patients with diabetic retinopathy [ 36 ]. miR-210 is also associated with diabetes mellitus and diabetic retinopathy, reportedly by mediating functional responses in endothelial cell function [ 37 ]. In a study of 150 type 2 diabetic patients, Yin et al found that serum miR-210 was increased in patients with diabetic retinopathy and was positively correlated with HbA1c, HOMA-IR, and fasting plasma glucose [ 37 ].…”

“…miR-210 is also associated with diabetes mellitus and diabetic retinopathy, reportedly by mediating functional responses in endothelial cell function [ 37 ]. In a study of 150 type 2 diabetic patients, Yin et al found that serum miR-210 was increased in patients with diabetic retinopathy and was positively correlated with HbA1c, HOMA-IR, and fasting plasma glucose [ 37 ]. They also report that miR-210 could differentiate patients with diabetic retinopathy from the healthy controls, highlighting the diagnostic potential of this miR [ 37 ].…”

“…In a study of 150 type 2 diabetic patients, Yin et al found that serum miR-210 was increased in patients with diabetic retinopathy and was positively correlated with HbA1c, HOMA-IR, and fasting plasma glucose [ 37 ]. They also report that miR-210 could differentiate patients with diabetic retinopathy from the healthy controls, highlighting the diagnostic potential of this miR [ 37 ]. Conversely, it was recently reported that miR-210 is lower in human RBCs in T2DM patients [ 38 ].…”

MicroRNA and diabetic retinopathy—biomarkers and novel therapeutics

“…Furthermore, proliferative DR (PDR) patients had higher levels than non-proliferative DR (NPDR) patients. The differential upregulation suggests that increased serum miR-210 could be used to identify patients with higher risk for PDR from NPDR patients (39). Two miRNAs mostly associated with the risk of DR in patients with T1DM are miR-27b and miR-320a (40).…”

microRNAs in Human Adipose Tissue Physiology and Dysfunction

“…Moreover, the expression of miR-210 was upregulated in the SAT of obese individuals with normal glucose tolerance, compared to those diagnosed with type 2 diabetes mellitus (T2DM), suggesting its protective role in the development of obesity-related adipose tissue dysfunction [ 34 ]. However, in patients with T2DM, serum miR-210 concentration may serve as a diagnostic biomarker of diabetic retinopathy patients and may have the ability to predict disease development and severity [ 35 ].…”