miRNA-195 expression in the tumor tissues of female Brazilian breast cancer patients with operable disease

Abstract

OBJECTIVE: This study aimed to assess miRNA-195 expression in the tumor tissues from a cohort of Brazilian female breast cancer patients undergoing neoadjuvant chemotherapy (NAC) and evaluate its correlation with various clinicopathological markers. METHODS: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to evaluate the miRNA-195 expression in tumor tissues from a cohort of female breast cancer patients undergoing NAC. This expression was then correlated with the occurrence of several distinct breast cancer molecular subtypes and other clinicopathological variables. RESULTS: A total of 55 patients were included in this study, 28 (50.9%) of whom were treated using NAC. Tumor miRNA-195 expression was suppressed in breast cancer patients, regardless of their exposure to systemic treatments, histological grade, size, nodal status, and tumor-node-metastasis (TNM) staging. This was more pronounced in luminal and triple-negative patients, and patient’s response to NAC was correlated with an increase in miRNA-195 expression. CONCLUSION: miRNA-195 is downregulated in the tumor tissues of Brazilian breast cancer patients regardless of NAC exposure; this reinforces its role as a tumor suppressor and a potential biomarker for chemotherapy response.

MicroRNAs: Important Players in Breast Cancer Angiogenesis and Therapeutic Targets

“…( Singh et al, 2015 ; Wang et al, 2016 ). In both BC cell lines and BC tissues, miR-195 is negatively linked to Insulin receptor substrate 1 (IRS1) ( de Sales et al, 2021 ). IRS1 expression is downregulated by induction miR-195 using a miR-195 oligo transfection method or infection with a lentivirus containing the miR-195 gene ( Wang et al, 2016 ; Lai et al, 2018 ).…”