miR-382 functions as a tumor suppressor against esophageal squamous cell carcinoma

Feng, Jie; Qi, Bo; Guo, Ling; Chen, Lingyun; Wei, Xiufeng; Liu, Yuzhen; Zhao, Baosheng

doi:10.3748/wjg.v23.i23.4243

Cited by 22 publications

(21 citation statements)

References 21 publications

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“…A clear understanding of the relationship between miRNA and the biological behaviour of malignant cells would render a novel index for early diagnosis, progress monitoring, and prognosis judgement . Previous studies have indicated that multiple members from miR‐382 family were implicated in the inhibition of malignancies, such as colorectal cancer, liver cancer, and esophageal squamous cell carcinoma . From a mechanistic point of view, miR‐382 impedes the progression of NSCLC by repressing SETD8 expression .…”

Section: Discussionmentioning

confidence: 99%

Circ_0001658 promotes the proliferation and metastasis of osteosarcoma cells via regulating miR‐382‐5p/YB‐1 axis

Wang

et al. 2019

Cell Biochemistry & Function

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Circular RNA (circRNA) can modulate the gene expression via sponging microRNA (miRNA) in multiple malignancies. Nevertheless, the detailed function of circ_0001658 in osteosarcoma (OS) and its regulatory mechanism remain elusive.Real-time PCR was carried out to detect the expressions of circ_0001658, miR-382-5p, and Y box-binding protein 1 (YB-1) mRNA in OS tissues. Besides, western blot was done to monitor YB-1 expression in OS cells. Chi-squared test was used to analyse the correlation between circ_0001658 and clinicopathologic characteristics of OS patients. CCK8 assay, colony formation assay, flow cytometry, and transwell assay were performed to determine the function of circ_0001658. Moreover, dualluciferase reporter gene assay was done to verify the targeting relationship between circ_0001658 and miR-382-5p. Compared with adjacent tissues, circ_0001658 displayed a significantly higher expression in OS tissues. Circ_0001658 could facilitate the proliferation, migration, and invasion of OS cells and impede apoptosis by sponging miR-382-5p. Further, circ_0001658 was proved to directly and negatively regulate the expression of miR-382-5 while positively modulate the expression of YB-1. Circ_0001658 promotes the proliferation and metastasis of OS cells via modulating miR-382-5p/YB-1 axis. Significance of the study:In recent years, the expression and function of circular RNA in cancer have been the focus of tumour research. The study on circular RNA helps elucidate the molecular pathology of tumorigenesis and cancer progression.This study demonstrated that circ_0001658 promotes the proliferation and metastasis of OS cells via modulating miR-382-5p/YB-1 axis. To our best knowledge, this work is the first to study the expression and function of circ_0001658 in cancer. K E Y W O R D Scirc_0001658, miR-382-5p, osteosarcoma, YB-1 | INTRODUCTIONOsteosarcoma (OS) is a common primary malignant bone tumour among children and adolescents, frequently diagnosed in the distal femur, the proximal tibia, and the proximal humerus. 1,2 Though multiple treatments offer symptomatic relief and modest improvement in survival for OS patients, the long-term disease-free survival rate of patients remains unfavourable, only 60% to 75%. 3,4 To make matters worse, the 5-year survival rate was only 27% to 55% in patients with local relapsing and distant metastasis treated by the available therapy clinically. 5 Hence, it is imperative to probe into the detailed mechanism of OS.Circular RNA (circRNA) is a novel class of endogenous noncoding RNA, which exists widely and stably in mammalian cells without the function of encoding proteins. 6,7 Circ-RNA, unlike linear RNAs, features a closed-loop structure. 8 This distinctive structure contributes

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Section: Discussionmentioning

confidence: 99%

Circ_0001658 promotes the proliferation and metastasis of osteosarcoma cells via regulating miR‐382‐5p/YB‐1 axis

Wang

et al. 2019

Cell Biochemistry & Function

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“…MiR-382, a microRNAs in the chromosome 14q32 locus, has been shown to be involved in development, metastasis, and therapeutic resistance in multiple types of cancers. It functions as a tumor suppressor in colorectal cancer,8,9 ovarian cancer,10 esophageal squamous cell carcinoma,11,12 osteosarcoma,13,14 prostate cancer,15 melanoma,16 and non-small cell lung cancer 17. MiR-382 exhibits oncogenic properties in gastric cancer,18 hepatocellular carcinoma,19 and breast cancer 20.…”

Section: Introductionmentioning

confidence: 99%

<p>The role of miR-382-5p in glioma cell proliferation, migration and invasion</p>

Wang¹,

Chen²,

Xu³

et al. 2019

OTT

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Background: Dysregulation of a single miRNA can play an essential role in tumor development and progression. Recent studies have shown that miR-382-5p can function as an oncogene or as a tumor suppressor in different types of cancers. However, the role of miR-382-5p in glioma growth and expansion has not been characterized. Methods: Quantitative real time-PCR (qRT-PCR) was used to measure miR-382-5p levels in glioma tissues. The miR-382-5p mimics and inhibitors were employed to upregulate and downregulate miR-382-5p expression respectively in glioma cells. EdU assay was used to assess cell proliferation. Wound healing and Transwell assays were employed to evaluate cell migration and invasion. Western blot was used to measure the changes of epithelial-to-mesenchymal transition (EMT) markers and the potential miR-382-5p target genes. Results: We found that miR-382-5p levels were low in glioma tissues as determined by qRT-PCR. EdU assay showed that upregulation of miR-382-5p significantly decreased cell proliferation in both U87 and U251 cells. Wound healing rate was significantly decreased in response to miR-382-5p mimics and significantly increased in response to miR-382-5p inhibitors. Transwell migration assays further confirmed the inhibitory effects of miR-382-5p on the migration in both U251 and U87 cells. Transwell invasion assays showed that upregulation of miR-382-5p resulted in a remarkable decrease in the number of invading cells, whereas downregulation of miR-382-5p led to a significant increase in the numbers of invading U87 and U251 cells. In addition, overexpression of miR-382-5p decreased the protein levels of N-cadherin, Snail and Slug, and increased E-cadherin levels, in glioma cells. Furthermore, miR-382-5p levels negatively correlated with Y box-binding protein 1 (YBX1) in lower grade glioma tissues, and negatively regulated the expression of YBX1 in glioma cells. Conclusion: In summary, miR-382-5p inhibited proliferation, migration, invasion, and the EMT in glioma cells, possibly through targeting the oncogene YBX1.

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“…Apparently, COUP-TFII is at the center of a miRNA network that elegantly explain the loss of EMT. MiR-382 is an onco-suppressor in non-small-cell lung, ovarian, prostate and esophageal cancers [189][190][191][192], and it is downregulated in colorectal cancer where it inhibits tumor metastasis, blocking SP1, and sensitizes cancer cells to chemotherapy [193,194]. Interestingly, COUP-TFII potentiates the action of SP1 in angiogenesis and development [92,94], recognizing SP1 response elements and miR-382 directly binds COUP-TFII 3 -UTR, knocking down the receptor, and hence downregulates two transcription factors that are demonstrated to synergize and induce EMT.…”

Section: The Gastrointestinal Cancers: Mostly An Oncogenementioning

confidence: 99%

COUP-TFII in Health and Disease

Polvani

Pepe

Milani

et al. 2019

Cells

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The nuclear receptors (NRs) belong to a vast family of evolutionary conserved proteins acting as ligand-activated transcription factors. Functionally, NRs are essential in embryogenesis and organogenesis and in adulthood they are involved in almost every physiological and pathological process. Our knowledge of NRs action has greatly improved in recent years, demonstrating that both their expression and activity are tightly regulated by a network of signaling pathways, miRNA and reciprocal interactions. The Chicken Ovalbumin Upstream Promoter Transcription Factor II (COUP-TFII, NR2F2) is a NR classified as an orphan due to the lack of a known natural ligand. Although its expression peaks during development, and then decreases considerably, in adult tissues, COUP-TFII is an important regulator of differentiation and it is variably implicated in tissues homeostasis. As such, alterations of its expression or its transcriptional activity have been studied and linked to a spectrum of diseases in organs and tissues of different origins. Indeed, an altered COUP-TFII expression and activity may cause infertility, abnormality in the vascular system and metabolic diseases like diabetes. Moreover, COUP-TFII is actively investigated in cancer research but its role in tumor progression is yet to be fully understood. In this review, we summarize the current understanding of COUP-TFII in healthy and pathological conditions, proposing an updated and critical view of the many functions of this NR.

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miR-382 functions as a tumor suppressor against esophageal squamous cell carcinoma

Cited by 22 publications

References 21 publications

Circ_0001658 promotes the proliferation and metastasis of osteosarcoma cells via regulating miR‐382‐5p/YB‐1 axis

Circ_0001658 promotes the proliferation and metastasis of osteosarcoma cells via regulating miR‐382‐5p/YB‐1 axis

<p>The role of miR-382-5p in glioma cell proliferation, migration and invasion</p>

COUP-TFII in Health and Disease

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