Circ_0001658 promotes the proliferation and metastasis of osteosarcoma cells via regulating miR‐382‐5p/YB‐1 axis

Abstract

Circular RNA (circRNA) can modulate the gene expression via sponging microRNA (miRNA) in multiple malignancies. Nevertheless, the detailed function of circ_0001658 in osteosarcoma (OS) and its regulatory mechanism remain elusive.Real-time PCR was carried out to detect the expressions of circ_0001658, miR-382-5p, and Y box-binding protein 1 (YB-1) mRNA in OS tissues. Besides, western blot was done to monitor YB-1 expression in OS cells. Chi-squared test was used to analyse the correlation between circ_0001658 and clinicopathologic characteristics of OS patients. CCK8 assay, colony formation assay, flow cytometry, and transwell assay were performed to determine the function of circ_0001658. Moreover, dualluciferase reporter gene assay was done to verify the targeting relationship between circ_0001658 and miR-382-5p. Compared with adjacent tissues, circ_0001658 displayed a significantly higher expression in OS tissues. Circ_0001658 could facilitate the proliferation, migration, and invasion of OS cells and impede apoptosis by sponging miR-382-5p. Further, circ_0001658 was proved to directly and negatively regulate the expression of miR-382-5 while positively modulate the expression of YB-1. Circ_0001658 promotes the proliferation and metastasis of OS cells via modulating miR-382-5p/YB-1 axis. Significance of the study:In recent years, the expression and function of circular RNA in cancer have been the focus of tumour research. The study on circular RNA helps elucidate the molecular pathology of tumorigenesis and cancer progression.This study demonstrated that circ_0001658 promotes the proliferation and metastasis of OS cells via modulating miR-382-5p/YB-1 axis. To our best knowledge, this work is the first to study the expression and function of circ_0001658 in cancer. K E Y W O R D Scirc_0001658, miR-382-5p, osteosarcoma, YB-1 | INTRODUCTIONOsteosarcoma (OS) is a common primary malignant bone tumour among children and adolescents, frequently diagnosed in the distal femur, the proximal tibia, and the proximal humerus. 1,2 Though multiple treatments offer symptomatic relief and modest improvement in survival for OS patients, the long-term disease-free survival rate of patients remains unfavourable, only 60% to 75%. 3,4 To make matters worse, the 5-year survival rate was only 27% to 55% in patients with local relapsing and distant metastasis treated by the available therapy clinically. 5 Hence, it is imperative to probe into the detailed mechanism of OS.Circular RNA (circRNA) is a novel class of endogenous noncoding RNA, which exists widely and stably in mammalian cells without the function of encoding proteins. 6,7 Circ-RNA, unlike linear RNAs, features a closed-loop structure. 8 This distinctive structure contributes

<p>Knockdown of CircCRIM1 Inhibits HDAC4 to Impede Osteosarcoma Proliferation, Migration, and Invasion and Facilitate Autophagy by Targeting miR-432-5p</p>

“…20 Circ_0001658 boosted cellular proliferative and metastatic abilities in OS via the modulation of miR-382-5p/YB-1 axis. 21 And circMMP9 was validated to facilitate the oncogenesis of OS by restricting miR-1265 expression to up-regulate CHI3L1. 22 In this chapter, we not only discovered the abnormal overexpression of circCRIM1 in OS tissues and cells but also ascertained that circCRIM1 worked as a tumor driver of OS for the first time.…”

Down-regulating lncRNA LINC00485 inhibits the development of osteosarcoma by regulating miR-361-5p/Twist1 axis

“…In recent years, multiple lines of evidence have clari ed that various miRNAs are implicated in regulating the osteosarcoma occurrence and development, and are abnormally expressed in cancer, functioning as oncogenes to exert effects [35]. For example, Circ_0001658 propels the proliferation and metastasis of osteosarcoma cells in way of regulating the miR-382-5p / YB-1 axis [36]. Knocking down miR-9 in human osteosarcoma cell lines inhibits their metastatic potential [37].…”

Emerging Roles and Potential Biological Value of CircRNA in Osteosarcoma

“…For example, compared with adjacent tissues, circ_0001658 displayed a significantly higher expression in OSA tissues. Hsa_circ_0001658 could impede apoptosis by sponging miR-382-5p and positively modulating Y-box binding protein 1 (YB-1) expression to facilitate the proliferation, migration, and invasion of OSA cells (33). Wu et al (44) revealed that increased circTADA2A expression in OSA tissue and cells promotes the progression and metastasis of OSA by sponging miR-203a-3p and by targeting oncogene cyclic AMP-responsive element-binding protein 3 (CREB3), both functionally and mechanistically.…”