miR-204–containing exosomes ameliorate GVHD-associated dry eye disease

Zhou, Tian; Lai, Peilong; Yang, Ziqi; Liu, Yan; Xu, Huiyi; Lin, Xiaojing; Ni, Biyan; Ju, Rong; Wei, Yi; Liang, Lingyi; Pei, Duanqing; Egwuagu, Charles E.; Liu, Xialin

doi:10.1126/sciadv.abj9617

Cited by 70 publications

(55 citation statements)

References 64 publications

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“…EVs secreted by MSC-derived from bone marrow, corneal stroma, human placenta, and induced pluripotent stem cells have been reported to accelerate corneal epithelial wound healing, decrease corneal epithelial defects, and reduce inflammatory cytokine production in animal models of corneal epithelial wound healing, corneal scarring, and DED associated with both Sjögren’s syndrome and graft-versus-host disease (GVHD) (GVHD-DED) [ 71 , 72 , 73 , 74 , 75 , 76 , 77 ].…”

Section: Corneal Epithelium Adsc Regeneration: Preclinical and Clinic...mentioning

confidence: 99%

“…Recently, patients affected by GVHD-DED were treated with exosomes isolated from MSCs in a prospective clinical trial [ 77 ] (Zhou 2022, Table 2 ). Fourteen patients, with 28 eyes in total, were included in the trial.…”

Section: Corneal Epithelium Adsc Regeneration: Preclinical and Clinic...mentioning

confidence: 99%

“…No adverse events related to cell products were reported. Symptoms (OSDI) and signs (TBUT, Schirmer’s test, and corneal staining) improved 14 days after treatment [ 77 ]. In addition, these authors suggested that exosomes reprogramme pro-inflammatory M1 macrophages towards immunosuppressive M2.…”

Section: Corneal Epithelium Adsc Regeneration: Preclinical and Clinic...mentioning

confidence: 99%

“…In addition, these authors suggested that exosomes reprogramme pro-inflammatory M1 macrophages towards immunosuppressive M2. These results showed remission of DED symptoms and an improvement in quality of life, suggesting that exosomes isolated from UC-MSCs can be a safe and innovative treatment for patients affected by GVHD-DED [ 77 ].…”

Section: Corneal Epithelium Adsc Regeneration: Preclinical and Clinic...mentioning

confidence: 99%

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Corneal Regeneration Using Adipose-Derived Mesenchymal Stem Cells

Barrio

Mata

Miguel

et al. 2022

Cells

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Adipose-derived stem cells are a subtype of mesenchymal stem cell that offers the important advantage of being easily obtained (in an autologous manner) from low invasive procedures, rendering a high number of multipotent stem cells with the potential to differentiate into several cellular lineages, to show immunomodulatory properties, and to promote tissue regeneration by a paracrine action through the secretion of extracellular vesicles containing trophic factors. This secretome is currently being investigated as a potential source for a cell-free based regenerative therapy for human tissues, which would significantly reduce the involved costs, risks and law regulations, allowing for a broader application in real clinical practice. In the current article, we will review the existing preclinical and human clinical evidence regarding the use of such adipose-derived mesenchymal stem cells for the regeneration of the three main layers of the human cornea: the epithelium (derived from the surface ectoderm), the stroma (derived from the neural crest mesenchyme), and the endothelium (derived from the neural crest cells).

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Section: Corneal Epithelium Adsc Regeneration: Preclinical and Clinic...mentioning

confidence: 99%

Section: Corneal Epithelium Adsc Regeneration: Preclinical and Clinic...mentioning

confidence: 99%

Section: Corneal Epithelium Adsc Regeneration: Preclinical and Clinic...mentioning

confidence: 99%

Section: Corneal Epithelium Adsc Regeneration: Preclinical and Clinic...mentioning

confidence: 99%

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Corneal Regeneration Using Adipose-Derived Mesenchymal Stem Cells

Barrio

Mata

Miguel

et al. 2022

Cells

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“…Therefore, MSC-Exos can be used for potential drug delivery vehicles in eye drops for SS-associated dry eyes [ 38 ]. MSC-Exos were also shown to be efficacious in treating graft-versus-host disease–associated dry eye disease via miR-204–mediated pathway [ 39 ]. Exosomes derived from mouse adipose-derived mesenchymal stem cells promote the reconstruction corneal epithelium, goblet cells, and tear secretion, reduce the levels of inflammatory cytokines IL-1β and interleukin-6 (IL-6) in the cornea and conjunctiva tissues of DED mouse models, suggesting that they could be used as a potential treatment [ 40 ].…”

Section: Research Progress Of Exosomes and Autophagy In Ocular Surfac...mentioning

confidence: 99%

Exosomes and autophagy in ocular surface and retinal diseases: new insights into pathophysiology and treatment

Liu

Yin

et al. 2022

Stem Cell Res Ther

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Background Ocular surface and retinal diseases are widespread problems that cannot be ignored in today’s society. However, existing prevention and treatment still have many shortcomings and limitations, and fail to effectively hinder the occurrence and development of them. Main body The purpose of this review is to give a detailed description of the potential mechanism of exosomes and autophagy. The eukaryotic endomembrane system refers to a range of membrane-bound organelles in the cytoplasm that are interconnected structurally and functionally, which regionalize and functionalize the cytoplasm to meet the needs of cells under different conditions. Exosomal biogenesis and autophagy are two important components of this system and are connected by lysosomal pathways. Exosomes are extracellular vesicles that contain multiple signaling molecules produced by multivesicular bodies derived from endosomes. Autophagy includes lysosome-dependent degradation and recycling pathways of cells or organelles. Recent studies have revealed that there is a common molecular mechanism between exosomes and autophagy, which have been, respectively, confirmed to involve in ocular surface and retinal diseases. Conclusion The relationship between exosomes and autophagy and is mostly focused on fundus diseases, while a deeper understanding of them will provide new directions for the pathological mechanism, diagnosis, and treatment of ocular surface and retinal diseases.

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Framework Nucleic Acids Combined with 3D Hybridization Chain Reaction Amplifiers for Monitoring Multiple Human Tear Cytokines

Lu,

Zhou,

Song

et al. 2024

Advanced Materials

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Existing tear sensors are difficult to perform multiplexed assays due to the minute amounts of biomolecules in tearsand the tiny volume of tears. Herein, we leverage DNA tetrahedral frameworks (DTFs) modified on the wireless portable electrodes to effectively capture three dimensional (3D)‐hybridization chain reaction (HCR) amplifiers for automatic and sensitive monitoring of multiple cytokines in human tears. The developed sensors allow the sensitive determination of various dry eye syndrome (DES)‐associated cytokines in human tears with the limit of detection down to 0.1 pg/mL, consuming as little as 3 microliters of tear fluid. Double‐blind testing of clinical DES samples using the developed sensor and commercial ELISA showed no significant difference between them. Compared with single‐biomarker diagnosis, the diagnostic accuracy of this sensor based on multiple biomarkers has improved by approximately 16%. The developed system offers the potential for tear sensors to enable personalized and accurate diagnosis of various ocular diseases.This article is protected by copyright. All rights reserved

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miR-204–containing exosomes ameliorate GVHD-associated dry eye disease

Abstract: MSC-exo eye drops alleviate GVHD-associated dry eye disease by shifting M1 macrophages to M2 via miR-204.

Cited by 70 publications

References 64 publications

Corneal Regeneration Using Adipose-Derived Mesenchymal Stem Cells

Corneal Regeneration Using Adipose-Derived Mesenchymal Stem Cells

Exosomes and autophagy in ocular surface and retinal diseases: new insights into pathophysiology and treatment

Framework Nucleic Acids Combined with 3D Hybridization Chain Reaction Amplifiers for Monitoring Multiple Human Tear Cytokines

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