miR‐17‐5p Regulates Differential Expression of NCOA3 in Pig Intramuscular and Subcutaneous Adipose Tissue

Abstract

Fat distribution affects economic value in pork production. Intramuscular adipose tissue (IMAT) improves meat quality, whereas subcutaneous adipose tissue (SCAT) is usually regarded as waste. In the present study, we analyzed IMAT/SCAT (I/S) ratios in each pig. Individuals selected from a population of 1200 Suhuai pigs were divided into two cohorts; those with high I/S ratios and those with low I/S ratios, and correlations between nuclear Receptor Co-activator 3 (NCOA3), a critical gene involved in regulating fat accumulation, and fat distribution were investigated. The ratio of IMAT NCOA3 to SCAT NCOA3 expression levels (NCOA3/NCOA3) was higher in the high I/S group compared with the low I/S group. The NCOA3 expression level in fat tissue was positively correlated with fat deposition. miR-17-5p was identified as a putative regulator of NCOA3 based on bioinformatics prediction analysis followed by gene expression analysis. The miR-17-5p/miR-17-5p ratio was negatively correlated with the NCOA3/NCOA3 ratio. The predicted relationship between miR-17-5p and NCOA3 was further verified by dual luciferase activity assays, qPCR, and western blots. Overexpression of miR-17-5p in intramuscular preadipocytes inhibited NCOA3 expression and reduced preadipocyte differentiation. FABP4 and PPARG expression were also significantly decreased, as was triglyceride content. Meanwhile, knockdown of miR-17-5p significantly increased NCOA3 expression and promoted intramuscular preadipocyte differentiation. Based on these results, we propose that differential expression of NCOA3 in pig intramuscular and subcutaneous adipose tissue is regulated by miR-17-5p.

Development and Genome Sequencing of a Laboratory-Inbred Miniature Pig Facilitates Study of Human Diabetic Disease

“…Rapidly evolving energy metabolism genes in BM are involved mainly in adipose deposition (such as AGPAT2, AGPAT4, AWAT1, and FABP6) and the growth and development of cardiac and skeletal muscles (such as FGFR2, FGFR4, and IGFBP4), reflecting a need to enhance the efficiency of biomass production under the nutrient-restricted feeding condition. Conversely, energy metabolism PSGs in Duroc are involved mainly in diabetic diseases, including eight PSGs playing important roles in resistance to diabetes (GAPT4, ZNF608, and BBS2) (Nishimura et al, 2001;Speliotes et al, 2010), atherosclerosis (SERPINE1) (Koch et al, 2010), insulin secretion (UCP2) (Bordone et al, 2006), and energy expenditure (GPAM, PRKCA, and NCOA3) (Han et al, 2017;Yu et al, 2017).…”

Integrative analysis of circRNAs, miRNAs, and mRNAs profiles to reveal ceRNAs networks in chicken intramuscular and abdominal adipogenesis

“…An increasing number of studies have shown that noncoding RNAs regulates various normal and pathological processes, such as microRNAs (miRNAs) [7][8][9][10] and long noncoding RNAs (lncRNAs) [11][12][13]. Although recent studies on the role of miRNAs and lncRNAs in adipogenesis have reached a mature stage, information on the functional roles of other classes of non-coding RNAs, such as circular RNAs (circRNAs) remains scanty.…”

Integrative analysis of circRNAs, miRNAs, and mRNAs profiles to reveal ceRNAs networks in chicken intramuscular and abdominal adipogenesis

“…An increasing number of studies have shown that non-coding RNAs regulates various normal and pathological processes, such as microRNAs (miRNAs) [7][8][9][10] and long non-coding RNAs (lncRNAs) [11][12][13]. Although recent studies on the role of miRNAs and lncRNAs in adipogenesis have reached a mature stage, information on the functional roles of other classes of non-coding RNAs, such as circular RNAs (circRNAs) remains scanty.…”