Integrative analysis of circRNAs, miRNAs, and mRNAs profiles to reveal ceRNAs networks in chicken intramuscular and abdominal adipogenesis

Zhang, Meng; Han, Yu; Zhai, Yanhui; Ma, Xiangfei; An, Xinglan; Zhang, Sheng; Li, Ziyi

doi:10.1186/s12864-020-07000-3

Cited by 28 publications

(24 citation statements)

References 53 publications

(67 reference statements)

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“…In our previous research, LPIN1 , downregulated by miR-429 in high-fat group abdominal fat tissues (the same individuals used in this study), inhibited lipid droplet formation. For epigenetics, miRNA play a pivotal role in the chicken lipid metabolic process ( Chen et al, 2019 ; Sun et al, 2019 ; Zhang et al, 2020a ). In this study, we also performed a small RNA sequencing and obtained 245 DESs.…”

Section: Discussionmentioning

confidence: 99%

Whole Transcriptome Analysis Reveals a Potential Regulatory Mechanism of LncRNA-FNIP2/miR-24-3p/FNIP2 Axis in Chicken Adipogenesis

Guo

Chao

Huang

et al. 2021

Front. Cell Dev. Biol.

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Lipid biosynthesis is a complex process, which is regulated by multiple factors including lncRNA. However, the role of lncRNA in chicken abdominal fat accumulation is still unclear. In this research, we collected liver tissues from six high abdominal fat rate Sanhuang broilers and six low abdominal fat rate Sanhuang broilers to perform lncRNA sequencing and small RNA sequencing. A total of 2,265 lncRNAs, 245 miRNAs, and 5,315 mRNAs were differently expressed. Among of them, 1,136 differently expressed genes were enriched in the metabolic process. A total of 36 differently expressed genes, which were considered as differently expressed lncRNAs’ targets, were enriched in the metabolic process. In addition, we also found out that eight differently expressed miRNAs could target 19 differently expressed genes. FNIP2 and PEX5L were shared in a cis-regulatory network and a differently expressed miRNA target relationship network. LncRNA-FNIP2/miR-24-3p/FNIP2 axis was considered as a potential candidate that may participate in lipid synthesis. Experimentally, the objective reality of lncRNA-FNIP2/miR-24-3p/FNIP2 axis was clarified and the regulation effect of lncRNA-FNIP2/miR-24-3p/FNIP2 axis on synthesis was validated. In brief, our study reveals a potential novel regulatory mechanism that lncRNA-FNIP2/miR-24-3p/FNIP2 axis was considered as being involved in lipid synthesis during chicken adipogenesis in liver.

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Section: Discussionmentioning

confidence: 99%

Whole Transcriptome Analysis Reveals a Potential Regulatory Mechanism of LncRNA-FNIP2/miR-24-3p/FNIP2 Axis in Chicken Adipogenesis

Guo

Chao

Huang

et al. 2021

Front. Cell Dev. Biol.

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“…circRNAs are very abundant in pig muscle, adipose tissue, and liver, and are expressed dynamically in a spatiotemporal manner [ 90 ]. circLCLAT1, circFNDC3AL, circCLEC19A, and circARMH1 regulate miRNAs through the PPAR pathway to influence the differentiation of chicken adipose cells and tissue-specific adipose deposition [ 91 ]. circ-PLXNA1 is mainly expressed in duck adipose tissue and the liver.…”

Section: Circrnas Related To Adipocyte Metabolismmentioning

confidence: 99%

Emerging functions of circular RNA in the regulation of adipocyte metabolism and obesity

Zhang

Tian

et al. 2022

Cell Death Discov.

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As noncoding RNAs, circular RNAs (circRNAs) are covalently enclosed endogenous biomolecules in eukaryotes that have tissue specificity and cell specificity. circRNAs were once considered a rare splicing byproduct. With the development of high-throughput sequencing, it has been confirmed that they are expressed in thousands of mammalian genes. To date, only a few circRNA functions and regulatory mechanisms have been verified. Adipose is the main tissue for body energy storage and energy supply. Adipocyte metabolism is a physiological process involving a series of genes and affects biological activities in the body, such as energy metabolism, immunity, and signal transmission. When adipocyte formation is dysregulated, it will cause a series of diseases, such as atherosclerosis, obesity, fatty liver, and diabetes. In recent years, many noncoding RNAs involved in adipocyte metabolism have been revealed. This review provides a comprehensive overview of the basic structure and biosynthetic mechanism of circRNAs, and further discusses the circRNAs related to adipocyte formation in adipose tissue and liver. Our review will provide a reference for further elucidating the genetic regulation mechanism of circRNAs involved in adipocyte metabolism.

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“…During the different differentiation stages of Chinese Erhualian pigs, the expression of circRNAs was changed in subcutaneous fat tissues [ 18 ]. In chickens, circRNA may affect adipogenesis by regulating miRNA-, PPAR- and fatty acid metabolism-related pathways, such as circLCLAT1, circFNDC3AL, circCLEC19A and circARMH1 [ 19 ]. In ducks, circ-PLXAN1 can affect the differentiation of duck adipocytes, which regulate the corresponding genes and participate in fat deposition by binding miR-214 [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Sus_circPAPPA2 Regulates Fat Deposition in Castrated Pigs through the miR-2366/GK Pathway

et al. 2022

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CircRNAs play an important role in fat deposition, and testosterone-deficient boars exhibit significantly increased fat deposition; however, the mechanism by which testosterone regulates fat deposition through circRNAs remains unclear. In this study, circRNA-seq of backfat and abdominal fat from castrated and intact full-sib Yorkshire pigs was performed. The GO and KEGG enrichment analyses revealed that the host genes of the dorsal DE circRNAs were mainly involved in fatty acid transport, while in abdominal tissues, these genes were mainly involved in adipogenesis and inflammation. The interaction among sus_circPAPPA2, ssc-miR-2366 and GK was verified by dual fluorescence experiments and in porcine preadipocytes. The overexpression of sus_circPAPPA2 significantly inhibited the differentiation of preadipocytes. The expression of sus_circPAPPA2 was increased after adding 100 nM of testosterone, and preadipocyte differentiation was significantly inhibited. Testosterone can affect preadipocyte differentiation by upregulating the expression of sus_circPAPPA2, sponging miR-2366 and regulating the expression of genes, such as GK. These results indicate that testosterone can regulate the expression of adipocyte differentiation- and lipid metabolism-related genes by regulating the expression of circRNA, and ceRNA networks are different in the testosterone regulation of adipose deposition in different parts. This study provides basic data enhancing the understanding of the interaction between the hormone environment and mir-2366/GK to regulate trait performance in pigs.

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Integrative analysis of circRNAs, miRNAs, and mRNAs profiles to reveal ceRNAs networks in chicken intramuscular and abdominal adipogenesis

Cited by 28 publications

References 53 publications

Whole Transcriptome Analysis Reveals a Potential Regulatory Mechanism of LncRNA-FNIP2/miR-24-3p/FNIP2 Axis in Chicken Adipogenesis

Whole Transcriptome Analysis Reveals a Potential Regulatory Mechanism of LncRNA-FNIP2/miR-24-3p/FNIP2 Axis in Chicken Adipogenesis

Emerging functions of circular RNA in the regulation of adipocyte metabolism and obesity

Sus_circPAPPA2 Regulates Fat Deposition in Castrated Pigs through the miR-2366/GK Pathway

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