MiR-148a-3p suppresses cell proliferation, migration and invasion by targeting PIK3CA in human osteosarcoma cells

Wang, Xu-Ke; Zhou, Yingjie; Zhao, Gang; Zheng, Huai-Liang

doi:10.18632/oncotarget.23931

Cited by 4 publications

(3 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expressions of SNHG4 and miR-148a-3p in CC was reported for the first time in the present study. The dysregulation of miR-148a-3p expression is involved in the progression of various tumors [31,32]. For instance, it was reported that miR-148a-3p was down-regulated in laryngeal squamous cell carcinoma [33].…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Long non-coding RNA SNHG4 promotes cervical cancer progression through regulating c-Met via targeting miR-148a-3p

Jiang

Wijayakulathilaka

2019

Cell Cycle

View full text Add to dashboard Cite

Long non-coding RNA (lncRNA) SNHG4 has been shown to be associated with the development of a variety of cancers. The purpose of this study was to investigate the effect of SNHG4 on cervical cancer (CC) and the corresponding mechanism. The qRT-PCR was used to determine the expressions of SNHG4 and miR-148a-3p in CC cell lines and tissues. Cell apoptosis and proliferation were measured by flow cytometry and MTT assay, respectively. The interaction between SNHG4, miR-148a-3p and c-Met was verified by bioinformatics, dual-luciferase reporter gene and RNA immunoprecipitation (RIP), and the effect of SNHG4 on the growth of CC tumor in vivo was explored. The expression of SNHG4 was increased in both CC cell lines and tissues, while the expression of miR-148a-3p was down-regulated. Meanwhile, silencing SNHG4 remarkably inhibited CC cell proliferation and promoted apoptosis. In addition, miR-148a-3p was a direct target gene of SNHG4, and down-regulation of miR-148a-3p could observably reverse the effect of silencing SNHG4 on the proliferation and apoptosis of CC cells. More importantly, SNHG4 could up-regulate the expression of c-Met by targeting and interacting with miR-148a-3p. Finally, in vivo experiments confirmed that silence SNHG4 down-regulated the expression of c-Met by promoting miR-148a-3p, and ultimately suppressed the growth of CC tumor in vivo. In conclusion, SNHG4 could be used as a competitive endogenous RNA to bind to miR-148a-3p, thereby up-regulating the expression of c-Met and ultimately promoting the progression of CC, which provided a potential therapeutic target for the targeted treatment of CC.

show abstract

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Long non-coding RNA SNHG4 promotes cervical cancer progression through regulating c-Met via targeting miR-148a-3p

Jiang

Wijayakulathilaka

2019

Cell Cycle

View full text Add to dashboard Cite

show abstract

“…Osteosarcoma (OS) is the most common type of primary malignant bone tumor and is characterized by complex genetic changes and resistance to conventional treatments . Although the incidence of OS is relatively low (about 6% of all childhood cancers) compared with other malignant tumors, the 5‐year overall survival rate of OS remains poor .…”

Section: Introductionmentioning

confidence: 99%

“…Osteosarcoma (OS) is the most common type of primary malignant bone tumor and is characterized by complex genetic changes and resistance to conventional treatments. 1 Although the incidence of OS is relatively low (about 6% of all childhood cancers) compared with other malignant tumors, the 5-year overall survival rate of OS remains poor. 2 As current research failed to obtain remarkable achievements in treatment outcomes, it is urgent for us to explore new therapeutic targets and novel approaches for the treatment of OS.…”

Section: Introductionmentioning

confidence: 99%

LncRNA DLX6‐AS1 promotes tumor proliferation and metastasis in osteosarcoma through modulating miR‐641/HOXA9 signaling pathway

Zhang

Meng

Mei

et al. 2019

J of Cellular Biochemistry

View full text Add to dashboard Cite

Osteosarcoma (OS) is the most common primary malignant bone tumor. Recently, increasing evidence has shown that the long noncoding RNA (lncRNA) DLX6-AS1 (distal-less homeobox 6 antisense 1) plays significant roles in various types of cancers. However, the functions and underlying mechanisms of DLX6-AS1 have not been explored in OS yet. In this study, we assessed the expression of DLX6-AS1 in OS tissues and cell lines and explored the underlying molecular mechanisms. DLX6-AS1 was found to be significantly upregulated in OS tissues and OS cell lines. High expression of DLX6-AS1 was significantly correlated with advanced TNM stage, high tumor grade, and distant metastasis of patients with OS. Knockdown of DLX6-AS1 suppressed OS cell proliferation, invasion, and migration, and induced cell apoptosis. Knockdown of DLX6-AS1 also suppressed in vivo tumor growth. Bioinformatics and luciferase assay analysis showed that DLX6-AS1 functioned as a competing endogenous RNA (ceRNA) to negatively regulate miR-641 expression.Furthermore, miR-641 was found to target the 3′ untranslated region of homeobox protein Hox-A9 (HOXA9) and suppressed the expression of HOXA9.Mechanistic studies showed that DLX6-AS1 regulated OS cell proliferation, invasion, and migration via regulating HOXA9 by acting as a ceRNA for miR-641. Our results suggested that DLX6-AS1 functions as a ceRNA by targeting miR-641/HOXA9 signal pathway to suppress OS cell proliferation and metastasis. Our study may provide novel insights into understanding pathogenesis and development of OS. K E Y W O R D SceRNA, DLX6-AS1, HOXA9, miR-641, osteosarcoma

show abstract

A spotlight on the interplay of signaling pathways and the role of miRNAs in osteosarcoma pathogenesis and therapeutic resistance

Doghish

Hegazy

Ismail

et al. 2023

Pathology - Research and Practice

View full text Add to dashboard Cite

MiR-148a-3p suppresses cell proliferation, migration and invasion by targeting PIK3CA in human osteosarcoma cells

Cited by 4 publications

References 34 publications

RETRACTED ARTICLE: Long non-coding RNA SNHG4 promotes cervical cancer progression through regulating c-Met via targeting miR-148a-3p

RETRACTED ARTICLE: Long non-coding RNA SNHG4 promotes cervical cancer progression through regulating c-Met via targeting miR-148a-3p

LncRNA DLX6‐AS1 promotes tumor proliferation and metastasis in osteosarcoma through modulating miR‐641/HOXA9 signaling pathway

A spotlight on the interplay of signaling pathways and the role of miRNAs in osteosarcoma pathogenesis and therapeutic resistance

Contact Info

Product

Resources

About