LncRNA DLX6‐AS1 promotes tumor proliferation and metastasis in osteosarcoma through modulating miR‐641/HOXA9 signaling pathway

Zhang, Ning; Meng, Xin; Mei, Lijun; Zhao, Chedong; Chen, Wei

doi:10.1002/jcb.28426

Cited by 33 publications

(40 citation statements)

References 30 publications

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“…Previous studies showed that miR-641 served as a tumor suppressor in human lung cancer [17] and induced apoptosis of cervical cancer cells [18]. To study the function of miR-641, we checked its expression and found that miR-641 was apparently downregulated in OS tissues and cells, which was supported by Chen's research [19]. In-depth research showed that miR-641 was negatively regulated by circUBAP2 in OS cells and miR-641 depletion boosted proliferation and invasion of OS cells and promoted the process of EMT.…”

Section: Discussionsupporting

confidence: 56%

“…Human osteoblastic cell line (hFOB1. 19) and OS cell lines (U2OS and SaOS2) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). McCoy's 5A medium (Sigma, St Louis, MO, USA), containing 5% CO 2 and 10% fetal bovine serum (FBS) (Sigma), was utilized to culture cells.…”

Section: Samples and Cell Culturementioning

confidence: 99%

“…Growing papers have emphasized the core position of miRNAs in controlling cancer development [14][15][16]. MiR-641 has been reported to be dysregulated in many human cancers [17,18] and a recent report indicated that miR-641 was downregulated in OS tissues [19]. Nevertheless, the function of miR-641 in OS development needs to be further explored.…”

Section: Introductionmentioning

confidence: 99%

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Circular RNA circUBAP2 regulates proliferation and invasion of osteosarcoma cells through miR-641/YAP1 axis

Zhu

et al. 2020

Cancer Cell Int

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Background: Osteosarcoma (OS) is a common malignant bone cancer and is still a growing threat to young people. Circular RNAs (CircRNAs) are reported to be involved in the development of diverse human cancers. However, the role of circUBAP2 in OS progression is rarely reported. Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to detect the expression levels of circUBAP2 and miR-641 in OS tissues and cells. Cell Counting Kit-8 (CCK-8) assay was employed to check cell proliferation. The ability of cell invasion was evaluated by transwell assay. The protein levels of E-cadherin, Vimentin and Yes-associated protein 1 (YAP1) were measured by western blot. The starBase was used to predict binding sites between miR-641 and circUBAP2 or YAP1 and the dual-luciferase reporter assay was performed to verify the interaction. Results: The level of circUBAP2 was significantly upregulated in OS tissues and cells compared with normal tissues and cells, which was contrary to the expression of miR-641. Downregulation of circUBAP2 suppressed proliferation and invasion of OS cells and weakened the process of epithelial-mesenchymal transition (EMT). Moreover, miR-641 was a target of circUBAP2 and could bind to the 3′-untranslated region (3′UTR) of YAP1. In addition, overexpression of circUBAP2 or YAP1 reversed the inhibitory effects of miR-641 on proliferation and invasion of OS cells. Further research indicated that circUBAP2 regulated the expression of YAP1 by interacting with miR-641 in OS cells. Conclusion: Knockdown of circUBAP2 impeded proliferation and invasion of OS cells by downregulating the expression of YAP1 via sponging miR-641.

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Section: Discussionsupporting

confidence: 56%

Section: Samples and Cell Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Circular RNA circUBAP2 regulates proliferation and invasion of osteosarcoma cells through miR-641/YAP1 axis

Zhu

et al. 2020

Cancer Cell Int

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“…A total of 23 articles were identified for full review, 11 of which were excluded due to lack of necessary data or in vitro studies. Finally, the remaining 12 articles [8,9,11,[14][15][16][17][18][19][20][21][22] were included in this meta-analysis. These eligible studies were published between 2017 and 2019, and covered a total of 841 patients.…”

Section: Search Results and Basic Characteristics Of The Included Docmentioning

confidence: 99%

LncRNA DLX6-AS1 as a potential molecular biomarker in the clinicopathology and prognosis of various cancers: a meta-analysis

et al. 2020

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Objective: Recent studies have shown that distal-less homeobox 6 antisense 1 （DLX6-AS1） is aberrantly expressed in various cancers and is associated with poor prognosis. This meta-analysis is designed to investigate the effects of DLX6-AS1 expression on clinicopathological features and survival outcomes. Methods: All eligible studies were searched from Pubmed, Web of Science, Embase, the Cochrane Library, and Wanfang database, up to August 2019. The literature was selected according to the inclusion and exclusion criteria listed in this work, and the quality of each eligible study was assessed. Each patient’s clinicopathological features and survival data were analyzed using Stata12.0 software. Begg’s test and sensitivity analysis were also conducted. Results: A total of 12 articles were included, covering 841 patients. Results showed that high expression of DLX6-AS1 was significantly closely associated with poor overall survival in tumor patients (HR = 2.30, 95% CI: 1.70–3.09, P < 0.01). This meta-analysis also showed that overexpression of DLX6-AS1 was significantly associated with tumor stage (P < 0.01), tumor size (P < 0.01), lymph node metastasis (P < 0.01), and distant metastasis (P < 0.01). Begg’s test suggested no publication bias. Conclusion: This meta-analysis revealed that high expression of DLX6-AS1 was related to the advanced clinicopathological characteristics of human digestive system cancers (gastric cancer, esophageal cancer, colon cancer, pancreatic cancer and hepatocellular carcinoma) and other cancers such as ovarian cancer, osteosarcoma and non-small cell lung cancer, and DLX6-AS1 has important predictive value for poor prognosis. However, more studies are needed to further corroborate these findings.

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“…This regulation could effectively reverse the inhibitory effects of miRNA-98-5p on the proliferation, migration, invasion, cell cycle and apoptosis of OS cells. Another report showed that distal-less homeobox 6-AS1 could inhibit the proliferation and metastasis of OS cells by targeting the miRNA-641/HOXA9 signaling pathway and acting as a ceRNA ( 32 ). Sun et al ( 33 ), found that MALAT1 could regulate the proliferation and metastasis of OS cells by competitively binding with miRNA-34a/c-5p and miRNA-449a/b.…”

Section: Promotive Role Of Lncrnas In the Occurrence And Development mentioning

confidence: 99%

Research progress regarding the role of long non‑coding RNAs in osteosarcoma (Review)

Wang

et al. 2020

Oncol Lett

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Osteosarcoma is a malignant tumor that occurs in children and adolescents. Although treatments for osteosarcoma have improved, the likelihood of survival remains low for most patients with metastasis and recurrence. Elucidating the mechanism underlying the development of osteosarcoma and chemotherapy resistance will be important to improve diagnosis and treatment. Long non-coding RNAs (lncRNAs), which are longer than 200 nucleotides in length and do not encode for proteins, have been shown to play a regulatory role in the occurrence and development of osteosarcoma, and are expected to serve as biomarkers and molecular targets. This review discusses the progress in the study of the role of lncRNAs in osteosarcoma, and highlights the recent developments in this field. Contents

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LncRNA DLX6‐AS1 promotes tumor proliferation and metastasis in osteosarcoma through modulating miR‐641/HOXA9 signaling pathway

Cited by 33 publications

References 30 publications

Circular RNA circUBAP2 regulates proliferation and invasion of osteosarcoma cells through miR-641/YAP1 axis

Circular RNA circUBAP2 regulates proliferation and invasion of osteosarcoma cells through miR-641/YAP1 axis

LncRNA DLX6-AS1 as a potential molecular biomarker in the clinicopathology and prognosis of various cancers: a meta-analysis

Research progress regarding the role of long non‑coding RNAs in osteosarcoma (Review)

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