miR-148a-3p promotes rabbit preadipocyte differentiation by targeting PTEN

Abstract

Although emerging data support crucial roles for microRNAs (miRNAs) during adipogenesis, the detailed mechanisms remain largely unknown. In this study, it was shown that in rabbits, levels of miR-148a-3p not only increased in white adipose tissue during early stages of growth but also during in vitro cultured preadipocyte differentiation. Furthermore, overexpression of miR-148a-3p significantly upregulated the mRNA levels of PPARγ, C/EBPα, and FABP4, as well as the protein levels of PPARγ, as indicated by qPCR and western blotting analyses. Overexpression of miR-148a-3p also promoted intracellular triglyceride accumulation. In contrast, downregulation of miR-148a-3p inhibited the differentiation of rabbit preadipocytes. Next, based on target gene prediction and a luciferase reporter assay, we further demonstrated that miR-148a-3p directly targeted one of the 3' untranslated regions of PTEN. Finally, it was observed inhibition of PTEN by siRNA promoted rabbit preadipocyte differentiation. Taken together, our results suggested that miR-148a-3p could be involved in regulating rabbit preadipocyte differentiation through inhibiting expression of PTEN, which further highlighted the importance of miRNAs during adipogenesis.

Inhibition of miR‐148a‐3p resists hepatocellular carcinoma progress of hepatitis C virus infection through suppressing c‐Jun and MAPK pathway

“…Similar results were also carried out in gastric cancer and HCC and miR‐148a was found up‐regulated in glioblastoma and chordomas . In our study, we figured c‐Jun as the target protein of miR‐148a, and it was the first time to combine miR‐148a and c‐Jun .…”

“…Lifers revealed miR‐148a, down‐regulated in human pancreatic ductal adenocarcinomas, regulated cell survival via CDC25B, a conserved dual specificity phosphatase which was significant in appropriate cell cycle . Similar results were also carried out in gastric cancer and HCC and miR‐148a was found up‐regulated in glioblastoma and chordomas .…”

“…MiRNA is a length of 19‐25 nt (nucleotides) noncoding RNAs . MiRNAs have a regulation in cell proliferation, differentiation, apoptosis and other cellular processes via enhancing or inhibiting the expression of many mRNAs . Numerous previous studies have manifested that miR‐148a‐3p was involved in laryngeal squamous cell carcinoma (LSCC), bladder cancer, gastric cancer and other several cancers .…”

“…13 MiRNAs have a regulation in cell proliferation, differentiation, apoptosis and other cellular processes via enhancing or inhibiting the expression of many mRNAs. [14][15][16] Numerous previous studies have manifested that miR-148a-3p was involved in laryngeal squamous cell carcinoma (LSCC), bladder cancer, gastric cancer and other several cancers. [13][14][15] However, there is a gap in the study of miR-148a-3p in the field of HCV, especially related to miRNA.…”

ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2

“…For example, Wang et al 24 confirmed that TGFBR1 and TGFBR2 are direct targets of miR-204 in fetal human retinal pigment epithelium using luciferase reporter assays. 31 It has also been previously reported that miR-204 is involved in cell proliferation and apoptosis, as Xin et al 32 found that this miRNA suppresses glioblastoma cell proliferation, migration, and invasion by targeting CYP27A1. Flores-Perez et al 26 found that miR-204 regulates tumor angiogenesis by targeting ANGPT1 and TGFBR2 in breast cancer.…”