ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2

Abstract

MicroRNAs (miRNAs) take part in a variety of biological processes by regulating target genes. Transforming growth factor β receptor 1 (TGFBR1) and TGFBR2 are crucial members of the TGF‐β family and are serine/threonine kinase receptors. The aim of this study was to explore the functions of ssc‐miR‐204 in porcine preadipocyte differentiation and apoptosis with regard to the TGFβ/Smad pathway. We identified miRNAs predicted to target TGFBR1 and TGFBR2 using a database and selected ssc‐miR‐204 as a candidate miRNA. ssc‐miR‐204 overexpression dramatically reduced the levels of TGFBR1 and TGFBR2. However, after transfection with ssc‐miR‐204 inhibitor, TGFBR1 and TGFBR2 levels were dramatically increased. ssc‐miR‐204 overexpression dramatically promoted porcine preadipocyte differentiation and apoptosis. After transfection with ssc‐miR‐204 inhibitor, porcine preadipocyte differentiation and apoptosis were dramatically inhibited. After transfection with ssc‐miR‐204 mimics, Smad2, Smad3, Smad4, p‐Smad2, and p‐Smad3 protein levels significantly decreased, and adipogenesis was regulated by inhibiting the TGF‐β/Smad3 signaling pathway. Taken together, these results verified that ssc‐miR‐204 regulates porcine preadipocyte differentiation and apoptosis by targeting TGFBR1 and TGFBR2.

MiR-146a-5p targeting SMAD4 and TRAF6 inhibits adipogenensis through TGF-β and AKT/mTORC1 signal pathways in porcine intramuscular preadipocytes

“…Based on the above results, SMAD4 functions mostly as a positive regulator of cell proliferation. Moreover, the SMAD4 and TGF-β signaling pathways play important roles in miRNA regulation of cell proliferation [41,42]. Notably, miR-183 promotes preadipocyte adipogenesis by suppressing SMAD4 expression in goats [43], and myostatin/ SMAD4 signaling inhibits 3T3-L1 cell differentiation [44].…”

MiR-146a-5p Targeting SMAD4 and TRAF6 Inhibits Adipogenensis Through TGF-β and NF-κB Signal Pathways in Porcine Intramuscular Preadipocytes

“…Based on above results, SMAD4 is mostly a positive regulator of cell proliferation. Moreover, The SMAD4 and TGF-β signaling pathways played important roles in the process of miRNA regulating cell proliferation, respectively [36,37]. Here, we revealed that miR-146a-5p targeting the SMAD4 was identi ed as a novel miRNA that repressed proliferation of porcine intramuscular preadipocytes via the TGF-β signaling pathway.…”

High-fat diet-induced gut microbiota alteration promotes lipogenesis by butyric acid/miR-204/ACSS2 axis in chickens

“…Our findings revealed that the highly expressed DEmiRNA miR-204 exhibited the most significant expression alteration subsequent to gut microbiota depletion. Previous studies have reported that miR-204 regulates the differentiation of mammalian preadipocytes, thereby influencing fat deposition ( Jin et al, 2019 ; Zhang et al, 2020 ). These results suggest that miR-204 may be a crucial target of gut microbiota in regulating the lipogenesis of chickens.…”