miR-144-3p inhibits cell proliferation of colorectal cancer cells by targeting BCL6 via inhibition of Wnt/β-catenin signaling

Sun, Naihui; Liang, Zhen; Zhang, Chongguang; Yuan, Yuan

doi:10.1186/s11658-020-00210-3

Cited by 50 publications

(36 citation statements)

References 33 publications

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“…MicroRNAs (miRNAs, miRs) are endogenously expressed small non-coding RNAs that generally regulate gene expression by binding to the 3ʹ untranslated region (3ʹ-UTR) of their targets, resulting in silencing [ 13 ]. Numerous studies have shown that they play crucial roles in human cancer development, with particular impact on cancer cell proliferation, differentiation, cell cycle and apoptosis [ 14 – 16 ]. Thus, they’re involved in regulating the progression and metastasis of tumors [ 17 – 19 ].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-296-5p inhibits cell metastasis and invasion in nasopharyngeal carcinoma by reversing transforming growth factor-β-induced epithelial–mesenchymal transition

Chen

Luo

et al. 2020

Cell Mol Biol Lett

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Aim To explore the effect of miR-296-5p on the metastasis of nasopharyngeal carcinoma (NPC) cells and investigate the underlying mechanism. Methods The expressions of miR-296-5p in NPC tissues and cells were determined using GSE32920 database analysis and real-time PCR and miRNA microarray assays. An miR-296-5p mimic and inhibitor were transfected into NPC cells. Then, immunofluorescence imaging, scratch wound-healing, transwell migration and invasion assays were used to observe the effects of miR-296-5p on cell metastasis and invasion. Real-time PCR and western blotting were carried out to detect the expressions of genes and proteins related to epithelial–mesenchymal transition (EMT). A dual luciferase reporter assay was used to identify whether TGF-β is the target gene of miR-296-5p. Finally, TGF-β expression plasmids were transfected into NPC cells to verify the role of TGF-β in the miR-296-5p-mediated inhibition of nasopharyngeal carcinoma cell metastasis. Results Our results show that miR-296-5p inhibits the migratory and invasive capacities of NPC cells by targeting TGF-β, which suppresses EMT. Importantly, the miR-296-5p level was significantly lower in human NPC tissues than in adjacent normal tissues. It also negatively correlated with TGF-β and was significantly associated with the lymph node metastasis of patients with NPC. Conclusions Our findings show that miR-296-5p represses the EMT-related metastasis of NPC by targeting TGF-β. This provides new insight into the role of miR-296-5p in regulating NPC metastasis and invasiveness.

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Section: Introductionmentioning

confidence: 99%

MicroRNA-296-5p inhibits cell metastasis and invasion in nasopharyngeal carcinoma by reversing transforming growth factor-β-induced epithelial–mesenchymal transition

Chen

Luo

et al. 2020

Cell Mol Biol Lett

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“…Sun and co-workers [ 21 ] identified miR-144-3p as a new biomarker for CRC diagnosis and response to treatment. miR-144-3p was found downregulated and associated with CRC pathological stages in CRC patients.…”

Section: Canonical Wnt/β-catenin Pathway and Crcmentioning

confidence: 99%

“…Previous studies revealed that BCL6 is involved in the control of cell cycle progression and differentiation [ 22 , 23 ]. Indeed, miR-144-3p/ BCL6 co-operate to inhibit cellular proliferation, development, and progression of CRC by interfering with c-myc and cyclin D1 expression [ 21 ] ( Table 1 ).…”

Section: Canonical Wnt/β-catenin Pathway and Crcmentioning

confidence: 99%

The Wnt Signalling Pathway: A Tailored Target in Cancer

Koni

Pinnarò

Brizzi

2020

IJMS

115

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Cancer is one of the greatest public health challenges. According to the World Health Organization (WHO), 9.6 million cancer deaths have been reported in 2018. The most common cancers include lung, breast, colorectal, prostate, skin (non-melanoma) and stomach cancer. The unbalance of physiological signalling pathways due to the acquisition of mutations in tumour cells is considered the most common cancer driver. The Wingless-related integration site (Wnt)/β-catenin pathway is crucial for tissue development and homeostasis in all animal species and its dysregulation is one of the most relevant events linked to cancer development and dissemination. The canonical and the non-canonical Wnt/β-catenin pathways are known to control both physiological and pathological processes, including cancer. Herein, the impact of the Wnt/β-catenin cascade in driving cancers from different origin has been examined. Finally, based on the impact of Extracellular Vesicles (EVs) on tumour growth, invasion and chemoresistance, and their role as tumour diagnostic and prognostic tools, an overview of the current knowledge linking EVs to the Wnt/β-catenin pathway is also discussed.

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“…Survivin and Bcl-2 family proteins exhibit their anti-apoptotic functions in tumor cells. Additionally, a recent investigation revealed that miR-144-3p could inhibit proliferation in CRC cells by targeting B-celll ymphoma 6 (BCL6) via the inhibition of Wnt/β-catenin signaling [28]. Iwaya et al [29] reported that mTOR is the direct target of miR-144 in CRC cells, and miR-144 suppression of mTOR led to the inhibition of cell proliferation.…”

Section: Microrna-144 In Cancer Proliferation and Apoptosismentioning

confidence: 99%

MicroRNA-144: A novel biological marker and potential therapeutic target in human solid cancers

Zhou

Hongwu

et al. 2020

J. Cancer

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MicroRNAs (miRNAs) are a class of small non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. It has been reported that microRNA-144 (miR-144) is highly conserved and can combine complementarily with the 3'-UTRs of target gene mRNAs to inhibit mRNA translation or promote targeted mRNA degradation. MiR-144 is abnormally expressed and has been identified as a tumor suppressor in many types of solid tumors. Increasing evidence supports a crucial role for miR-144 in modulating physiopathologic processes, such as proliferation, apoptosis, invasion, migration and angiogenesis in different tumor cells. Apart from these functions, miR-144 can also affect drug sensitivity, cancer treatment and patient prognosis. In this review, we summarize the biological functions of miR-144, its direct targets and the important signal pathways through which it acts in relation to various tumors. We also discuss the role of miR-144 in tumor biology and its clinical significance in detail and offer novel insights into molecular targeting therapy for human cancers.

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miR-144-3p inhibits cell proliferation of colorectal cancer cells by targeting BCL6 via inhibition of Wnt/β-catenin signaling

Cited by 50 publications

References 33 publications

MicroRNA-296-5p inhibits cell metastasis and invasion in nasopharyngeal carcinoma by reversing transforming growth factor-β-induced epithelial–mesenchymal transition

MicroRNA-296-5p inhibits cell metastasis and invasion in nasopharyngeal carcinoma by reversing transforming growth factor-β-induced epithelial–mesenchymal transition

The Wnt Signalling Pathway: A Tailored Target in Cancer

MicroRNA-144: A novel biological marker and potential therapeutic target in human solid cancers

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