miR-142-5p regulates pancreatic cancer cell proliferation and apoptosis by regulation of RAP1A

Yao, Rong; Xu, Lijuan; Wei, Bin; Qian, Zhiguo; Wang, Jiru; Hui, Hongxia; Sun, Yuan

doi:10.1016/j.prp.2019.04.008

Cited by 29 publications

(17 citation statements)

References 25 publications

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“…Further dual-luciferase reporter assays and RIP assay confirmed the binding of miR-142-5p to hsa_circ_0137439. The miR-142-5p has been reported as a functional miRNA that could influence proliferation and migration in cancers, including pancreatic cancer, non-small cell lung cancer, osteosarcoma, and so on [23][24][25]. These previous studies reported that inhibition of miR-142-5p by hsa_circ_0137439 could suppress the proliferation and migration of breast cancer cells, which was coincided with our function experiments.…”

Section: Discussionsupporting

confidence: 89%

Identification of urinary hsa_circ_0137439 as a potential biomarker and tumor regulator of bladder cancer

Song¹,

Zhang²,

Zhu³

et al. 2020

neo

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Cell-free circular RNAs (circRNAs) stably and abundantly exist in body fluids. In this study we aimed to investigate the potential of urinary cell-free circRNAs as a novel class of non-invasive disease biomarkers for diagnosis of bladder cancer. Differentially expressed circRNAs from 10 normal and 10 bladder cancer urine samples were firstly detected by microarray. Hsa_circ_0137439 was then screened and validated in 30 normal and 116 bladder cancer samples. A receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of hsa_circ_0137439. The Kaplan-Meier method was used to evaluate the significance of hsa_circ_0137439 in the prognosis of bladder cancer. We found that hsa_circ_0137439 was significantly upregulated in bladder cancer samples. Moreover, increased expression of hsa_circ_0137439 was correlated with higher tumor stage, higher tumor grade, higher lymph node status, and history of muscle-invasive bladder cancer (MIBC). Also, urinary cell-free hsa_circ_0137439 could not only differentiate bladder cancer from normal controls but also distinguish MIBC from non-muscle-invasive bladder cancer (NMIBC). Additionally, hsa_circ_0137439 in urine supernatant could serve as an independent prognostic predicator of recurrence-free survival and overall survival for patients with bladder cancer. Cell assays showed that hsa_circ_0137439 knockdown contributed to the inhibition of cell proliferation and migration via hsa_circ_0137439/miR-142-5p/MTDH axis. In conclusion, urinary cell-free hsa_circ_0137439 could be a promising biomarker for tumor diagnosis and prognostic assessment of bladder cancer patients.

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Section: Discussionsupporting

confidence: 89%

Identification of urinary hsa_circ_0137439 as a potential biomarker and tumor regulator of bladder cancer

Song¹,

Zhang²,

Zhu³

et al. 2020

neo

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“…Our present study showed that upregulation of miR-142-5p resulted in clearly decreased apoptosis rate in OA chondrocytes along with significant changes in the expression levels of Bax, Bcl-2, cleaved caspase-3, and some inflammation-associated factors including IL-10, IL-1β, and TNF-α. It has been reported that miR-142-5p acts as a master regulator of osteoclast differentiation, cells proliferation, and apoptosis [32,33]. In pancreatic cancer cells, miR-142-5p has been found to target Ras-related protein Rap-1A (RAP1A) to downregulate p-ERK1/2 and phosphate p38 mitogen-activated protein kinases (p-p38) and control cell proliferation and apoptosis [33].…”

Section: Discussionmentioning

confidence: 99%

“…ese results suggest that miR-42-5p alleviates chondrocyte apoptosis, inflammation factor release, and ECM deposition even in chondrocytes with active SDF-1/ CXCR4 signaling, which provides rationale for clinical practice of treating OA patients with external miR-142-5p. On the other hand, since miR-142-5p is involved in many pathways such as regulating the PI3k/Akt/FoxO1 pathway via targeting PTEN in bone marrow-derived macrophages [32], more investigations are required to reveal the full mechanisms of the beneficial effects of miR-142-5p in OA chondrocytes, which might not be limited to targeting CXCR4. Our findings also support a link between OA development and the activation of MAPK signaling, the two major OA risk factors.…”

Section: Discussionmentioning

confidence: 99%

miR-142-5p as a CXCR4-Targeted MicroRNA Attenuates SDF-1-Induced Chondrocyte Apoptosis and Cartilage Degradation via Inactivating MAPK Signaling Pathway

Xiang

Liu

Yang

et al. 2020

Biochemistry Research International

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Osteoarthritis (OA) is a chronic joint function disorder with characteristics of chondrocytes reduction and extracellular matrix (ECM) components destruction. MicroRNAs (miRNAs) and the SDF-1/CXCR4 axis are essential factors of chondrocyte apoptosis and ECM degeneration. However, very few studies have investigated the correlation between miRNAs and the SDF-1/CXCR4 axis in osteoarthritis so far. Here, through miRNAs microarray and bioinformatics analyses, we identified miR-142-5p as a CXCR4-targeted and dramatically downregulated miRNA in cartilage from OA patients, as well as in SDF-1-induced OA chondrocytes in vitro. In SDF-1-treated primary human OA chondrocytes that were transfected with a miR-142-5p mimic or inhibitor, the expression of CXCR4 was found to be inversely correlated with the expression of miR-142-5p. The dual luciferase reporter assay further verified the target relationship between miR-142-5p and CXCR4. Overexpression of miR-142-5p alleviated OA pathology by suppressing chondrocyte apoptosis, even in CXCR4 overexpressed OA chondrocytes. This was associated with decreased cartilage matrix degradation, reduced cartilage inflammation, and inactivated MAPK signaling pathway. Our study suggests that upregulated expression of CXCR4-targeted miR-142-5p can inhibit apoptosis, inflammation, and matrix catabolism and inactivate the MAPK signaling pathway in OA chondrocytes. Our work provides important insight into targeting miR-142-5p and the SDF-1/CXCR4 axis in OA therapy.

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“…MiR-142-5p has been reported to be related to cell viability and apoptosis. In pancreatic cancer, miR-142-5p was reported to play the role of cancer suppressor through regulating pancreatic cancer cell proliferation and apoptosis [31]. Another study by Yang et al suggested that miR-142-5p functions as a growth promotive miRNA and plays an important role in neurogenic differentiation of adipose-derived stem cells (ADSCs) [32].…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective effects of miR-142-5p downregulation against isoflurane-induced neurological impairment

2020

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Background: Isoflurane can lead to neuron damage to the developing brain, resulting in learning and memory disability. The aim of this study was to investigate the role of miR-142-5p on isoflurane-induced neurological impairment. Methods: The Morris water maze (MWM) test was performed to evaluate spatial learning and memory of rats. The expression level of miR-142-5p was measured using qRT-PCR. MTT assay was used to calculate the viability of hippocampal neuronal cells. The cell apoptosis was analyzed using Flow cytometric assay. Results: Isoflurane treatment led to the increase of neurological function score and escape latency, and the reduction of time spent in the original quadrant in rats. The expression level of miR-142-5p was increased significantly in isoflurane-treated rats. MiR-142-5p downregulation protected against isoflurane-induced neurological impairment, which was reflected by the decrease of neurological function score and escape latency, and the increase of time spent in the original quadrant. In vitro, downregulation of miR-142-5p alleviated isoflurane-induced neuron cell viability inhibition, and relieved isoflurane-induced cell apoptosis. Conclusions: MiR-142-5p downregulation plays a neuroprotective role in protecting against isoflurane-induced neurological impairment through regulating neuron cell viability and apoptosis. It provides a theoretical basis for the investigation of the mechanism underlying the effect on isoflurane-induced neurological impairment.

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miR-142-5p regulates pancreatic cancer cell proliferation and apoptosis by regulation of RAP1A

Cited by 29 publications

References 25 publications

Identification of urinary hsa_circ_0137439 as a potential biomarker and tumor regulator of bladder cancer

Identification of urinary hsa_circ_0137439 as a potential biomarker and tumor regulator of bladder cancer

miR-142-5p as a CXCR4-Targeted MicroRNA Attenuates SDF-1-Induced Chondrocyte Apoptosis and Cartilage Degradation via Inactivating MAPK Signaling Pathway

Neuroprotective effects of miR-142-5p downregulation against isoflurane-induced neurological impairment

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