MiR-135b-5p promotes viability, proliferation, migration and invasion of gastric cancer cells by targeting Krüppel-like factor 4 (KLF4)

Chen, Zhi; Gao, Yongjian; Gao, Shengxiang; Song, Defeng; Feng, Yan

doi:10.5114/aoms.2019.87761

Cited by 39 publications

(26 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cox regression analysis in patients with non-small cell lung cancer. Numerous studies have indicated that miRNAs are involved in the pathogenesis of human cancer by regulating tumor cell processes, such as proliferation, migration and invasion (30)(31)(32). Therefore, the present study carried out further cellular experiments to uncover the functional role of miR-665 in NSCLC progression.…”

Section: Discussionmentioning

confidence: 91%

Upregulated miR‑665 expression independently predicts poor prognosis of lung cancer and facilitates tumor cell proliferation, migration and invasion

Xia

Zhu

et al. 2020

Oncol Lett

View full text Add to dashboard Cite

Non-small cell lung cancer (NSCLC) is one of the leading causes of global cancer-associated mortality. Aberrant microRNAs (miRs) have been reported to be involved in the pathogenesis of various cancer types. The present study aimed to investigate the expression profile and prognostic value of miR-665 in patients with NSCLC, and to analyze its functional role in tumor progression using NSCLC cells. Reverse transcription-quantitative PCR was used to estimate the expression levels of miR-665. Kaplan-Meier survival curves and Cox regression analysis were performed to evaluate the prognostic value of miR-665. The effects of miR-665 on NSCLC cell proliferation, migration and invasion were examined by cell transfection, and the target gene of miR-665 was explored. miR-665 expression was elevated in the tissue and cell samples of NSCLC. This increased miR-665 expression was associated with lymph node metastasis and TNM stage. An independent association between miR-665 and overall survival was identified in patients with NSCLC. When regulating the expression levels of miR-665 in vitro, NSCLC cell proliferation, migration and invasion were enhanced by overexpression of miR-665, but were inhibited by knockdown of miR-665. The luciferase activity results indicated that the protein tyrosine phosphatase receptor type B (PTPRB) was a direct target of miR-665 in NSCLC cells. The present study provided evidence for the clinical significance of a decreased expression of miR-665 in the prognosis of NSCLC. Upregulation of miR-665 contributed to tumor cell proliferation, migration and invasion by targeting PTPRB, suggesting the potential of miR-665 as a candidate therapeutic target for NSCLC treatment.

show abstract

Section: Discussionmentioning

confidence: 91%

Upregulated miR‑665 expression independently predicts poor prognosis of lung cancer and facilitates tumor cell proliferation, migration and invasion

Xia

Zhu

et al. 2020

Oncol Lett

View full text Add to dashboard Cite

show abstract

“…Indeed, our results suggest that many aberrantly expressed circRNAs regulated many genes function in cancer-related pathways ( Figures S3–S5 ). miR-135b-5p is also reported to target KLF4 [ 67 ], an important transcription factor in skin barrier formation [ 68 ]; a pathway that is disrupted in psoriasis.…”

Section: Discussionmentioning

confidence: 99%

Canonical and Interior Circular RNAs Function as Competing Endogenous RNAs in Psoriatic Skin

Liu

Frost

Bowcock

et al. 2021

IJMS

View full text Add to dashboard Cite

(1) Background: Understanding the function of circular RNAs (circRNAs), a class of noncoding RNA, in psoriatic skin can provide important insights into the complex regulation of genes contributing to the pathogenesis of psoriasis. (2) Methods: A novel method was applied to RNA-seq datasets from 93 skin biopsy samples to comprehensively identify circRNAs of all types, i.e., canonical circRNAs from the intron-exon junctions of mRNAs and interior circRNAs (i-circRNAs) from the interior regions of exons, introns, and intergenic regions. Selected circRNAs were experimentally validated by qRT-PCR and Sanger sequencing. CircRNAs with abundant and differential expression were identified and their putative function as competing endogenous RNAs (ceRNAs) was analyzed by an integrated analysis of circRNAs, microRNAs, and mRNAs. (3) Results: With a comprehensive search using no information of splicing signals, we systematically identified 179 highly abundant circRNAs in psoriatic skin. Many of these were reported for the first time and many were differentially expressed in involved versus normal or uninvolved skin. Validation based on three additional RNA-seq datasets confirmed most of the identified circRNAs in psoriatic skin. Experimental analyses confirmed the expression of the well-known circRNA CDR1as, a canonical circRNA, and a novel i-circRNA in psoriasis. We also identified many circRNAs that may act as ceRNAs to regulate the expression of mRNA genes in psoriasis-related signaling pathways in psoriasis. (4) Conclusions: The result of the study suggested that circRNAs are abundant in psoriatic skin, have distinct characteristics, and contribute to psoriatic pathogenesis.

show abstract

“…A previous study has shown that overexpression of KLF4 will lead to a decrease in the expression of N-cadherin, MMP2, and MMP9 [ 34 ]. On the contrary, the loss of KLF4 weakened the inhibition of N-cadherin, MMP2, and MMP9, leading to increased tumor cell invasion and migration, thereby affecting survival and prognosis [ 35 ]. KLF5, in contrast to KLF4, showed high expression in gastrointestinal tumors.…”

Section: Discussionmentioning

confidence: 99%

The Partial Role of KLF4 and KLF5 in Gastrointestinal Tumors

Chen

Jian

et al. 2021

Gastroenterology Research and Practice

View full text Add to dashboard Cite

Background. KLF4 and KLF5 are members of the KLF transcription factor family, which play an important role in many gastrointestinal tumors. To gain a deeper insight into its function and role, bioinformatics was used to analyze the function and role of KLF4 and KLF5 in gastrointestinal tumors. Methods. Data were collected from several online databases. Gene Expression Profiling Interactive Analysis (GEPIA), UALCAN database analysis, Kaplan-Meier Plotter analysis, LOGpc system, the Pathology Atlas, and the STRING website were used to analyze the data. We download relevant data from TCGA and then perform GO enrichment and KEGG enrichment analysis. The effects of KLF5 on gastric cancer cell proliferation were measured by CCK-8 assay. The effect of KLF5 on the expression of CyclinD1 and MMP9 was detected by Western blot. Results. KLF4 and KLF5 were differentially expressed in normal and tumor tissues of the gastrointestinal tract, and their differential expression is related to several genes or pathways. KEGG analysis showed that KLF5 was coexpressed with endocytosis-related genes. KLF5 promotes the proliferation of gastric cancer cells and the expression of metastasis-related molecules. Conclusion. KLF4 and KLF5 are of great significance for developing gastrointestinal tumors and can be used as therapeutic targets.

show abstract

MiR-135b-5p promotes viability, proliferation, migration and invasion of gastric cancer cells by targeting Krüppel-like factor 4 (KLF4)

Cited by 39 publications

References 44 publications

Upregulated miR‑665 expression independently predicts poor prognosis of lung cancer and facilitates tumor cell proliferation, migration and invasion

Upregulated miR‑665 expression independently predicts poor prognosis of lung cancer and facilitates tumor cell proliferation, migration and invasion

Canonical and Interior Circular RNAs Function as Competing Endogenous RNAs in Psoriatic Skin

The Partial Role of KLF4 and KLF5 in Gastrointestinal Tumors

Contact Info

Product

Resources

About