Upregulated miR‑665 expression independently predicts poor prognosis of lung cancer and facilitates tumor cell proliferation, migration and invasion

Abstract

Non-small cell lung cancer (NSCLC) is one of the leading causes of global cancer-associated mortality. Aberrant microRNAs (miRs) have been reported to be involved in the pathogenesis of various cancer types. The present study aimed to investigate the expression profile and prognostic value of miR-665 in patients with NSCLC, and to analyze its functional role in tumor progression using NSCLC cells. Reverse transcription-quantitative PCR was used to estimate the expression levels of miR-665. Kaplan-Meier survival curves and Cox regression analysis were performed to evaluate the prognostic value of miR-665. The effects of miR-665 on NSCLC cell proliferation, migration and invasion were examined by cell transfection, and the target gene of miR-665 was explored. miR-665 expression was elevated in the tissue and cell samples of NSCLC. This increased miR-665 expression was associated with lymph node metastasis and TNM stage. An independent association between miR-665 and overall survival was identified in patients with NSCLC. When regulating the expression levels of miR-665 in vitro, NSCLC cell proliferation, migration and invasion were enhanced by overexpression of miR-665, but were inhibited by knockdown of miR-665. The luciferase activity results indicated that the protein tyrosine phosphatase receptor type B (PTPRB) was a direct target of miR-665 in NSCLC cells. The present study provided evidence for the clinical significance of a decreased expression of miR-665 in the prognosis of NSCLC. Upregulation of miR-665 contributed to tumor cell proliferation, migration and invasion by targeting PTPRB, suggesting the potential of miR-665 as a candidate therapeutic target for NSCLC treatment.

Circular RNA hsa-circ-000881 suppresses the progression of lung adenocarcinoma in vitro via a miR-665/PRICKLE2 axis

“…Liu et al also found that miR-665 expression was upregulated in small cell lung cancer tissues (29). Xia et al demonstrated miR-665 up-regulation in NSCLC tissues and cells, with the overexpression of miR-665 promoting cell proliferation, migration, and invasion (30). Likewise, we also found that miR-665 expression was significantly upregulated in LUAD.…”

MicroRNA‑665 regulates the proliferation, apoptosis and adhesion of gastric cancer cells by binding to cadherin 3

“…Nonetheless, miR-665 could facilitate the tumorigenesis of ovarian cancer cells by targeting SRC Kinase Signaling Inhibitor 1 (31). In addition, miR-665 overexpression enhanced the proliferation, migration and invasion of non-small cell lung cancer cells (16). Regarding GC, miR-665 has been documented in several studies to suppress the progression of GC (20,33).…”

“…Previous studies have revealed that miRNAs regulate cell proliferation, invasion and apoptosis, as well as serving a pivotal role in tumor progression (14,15). For instance, miR-665 has been associated with lung cancer progression by increasing cell proliferation, migration and invasion (16). Some studies have suggested that miR-665 may be used as a potential molecular target for various types of cancer, including pancreatic (17), breast (18) and ovarian carcinoma (19).…”

Correlation analysis of microribonucleic acid‐155 and microribonucleic acid‐29 with type 2 diabetes mellitus, and the prediction and verification of target genes

“…Apart from cardiovascular diseases, microRNAs are also associated with obesity‐associated tumorigenesis. Upregulation of microRNA665 expression independently predicts poor lung cancer prognosis, and promotes tumor cell proliferation, migration and invasion 24 . Wu et al 25 .…”