MiR-129-5p influences the progression of gastric cancer cells through interacting with <i>SPOCK1</i>

Liu, Yan; Sun, Kai; Liu, Yang; Liang, Jun; Cai, Kerui; Gui, Jinqiu

doi:10.1177/1010428317706916

Cited by 23 publications

(21 citation statements)

References 33 publications

(41 reference statements)

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“…For example, mRNAs like miR‐105 and miR‐592 were reported to inhibit glioma cell malignancy, while some other mRNAs like miR‐21 and miR‐183 promoted the metastasis of glioma cells . Previous studies had revealed the suppressive role of miR‐129‐5p in several malignancies such as chondrosarcoma, gastric cancer and laryngeal cancer . In our study, we attempted to elucidate the biological function of miR‐129‐5p in human glioma.…”

Section: Discussionmentioning

confidence: 98%

“…22,23 Previous studies had revealed the suppressive role of miR-129-5p in several malignancies such as chondrosarcoma, gastric cancer and laryngeal cancer. [24][25][26] In our study, we attempted to elucidate the biological function of miR-129-5p in human glioma. We revealed the down-regulation of miR-129-5p in human glioma tissues and cells and the remarkable suppressive effects of miR-129-5p F I G U R E 7 TGIF2 inhibited cell apoptosis and blocked cell cycle of U87 and U251.…”

Section: Tgif2 Stimulated Glioma Cell Multiplication and Metastasismentioning

confidence: 99%

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MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2

Diao

Jin

Huang

et al. 2018

J Cellular Molecular Medi

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This study purposed to explore the correlation between miR‐129‐5p and TGIF2 and their impacts on glioma cell progression. Differentially expressed miRNA was screened through microarray analysis. MiR‐129‐5p expression levels in glioma tissues and cells were measured by qRT‐PCR. CCK‐8 assay, flow cytometer, transwell assay and wound‐healing assay were employed to detect cell proliferation, apoptosis and cycle, invasiveness and migration, respectively. Dual‐luciferase reporting assay was performed to confirm the targeted relationship between miR‐129‐5p and TGIF2. The effects of TGIF2 expression on cell biological functions were also investigated using the indicated methods. Tumour xenograft was applied to explore the impact of miR‐129‐5p on tumorigenesis in vivo. MiR‐129‐5p expression was down‐regulated in both glioma tissues and glioma cells, while TGIF2 expression was aberrantly higher than normal level. Dual‐luciferase reporter assay validated the targeting relation between miR‐129‐5p and TGIF2. Overexpression of miR‐129‐5p or down‐regulation of TGIF2 inhibited the proliferation, invasion and migration capacity of glioma cells U87 and U251, and meanwhile blocked the cell cycle as well as induced cell apoptosis. MiR‐129‐5p overexpression repressed the tumour development in vivo. MiR‐129‐5p and TGIF2 had opposite biological functions in glioma cells. MiR‐129‐5p could inhibit glioma cell progression by targeting TGIF2, shining light for the development of target treatment for glioma.

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Section: Discussionmentioning

confidence: 98%

Section: Tgif2 Stimulated Glioma Cell Multiplication and Metastasismentioning

confidence: 99%

MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2

Diao

Jin

Huang

et al. 2018

J Cellular Molecular Medi

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“…28 In gastric cancer, miR-129-5p could inhibit the expression of SPOCK1, leading to the suppression of cancer cell processes. 20 Nonetheless, the target relationship between SPOCK1…”

Section: Discussionmentioning

confidence: 99%

MiR‐139‐5p, miR‐940 and miR‐193a‐5p inhibit the growth of hepatocellular carcinoma by targeting SPOCK1

Xiao

Luo

et al. 2019

J Cellular Molecular Medi

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The study was aimed to screen out miRNAs with differential expression in hepatocellular carcinoma (HCC), and to explore the influence of the expressions of these miRNAs and their target gene on HCC cell proliferation, invasion and apoptosis. MiRNAs with differential expression in HCC were screened out by microarray analysis. The common target gene of these miRNAs (miR‐139‐5p, miR‐940 and miR‐193a‐5p) was screened out by analysing the target genes profile (acquired from Targetscan) of the three miRNAs. Expression levels of miRNAs and SPOCK1 were determined by quantitative real time polymerase chain reaction (qRT‐PCR). The target relationships were verified by dual luciferase reporter gene assay and RNA pull‐down assay. Through 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2‐H‐tetrazolium bromide,thiazolyl blue tetrazolium bromide (MTT) and transwell assays and flow cytometry, HCC cell viability, invasion and apoptosis were determined. In vivo experiment was conducted in nude mice to investigate the influence of three miRNAs on tumour growth. Down‐regulation of miR‐139‐5p, miR‐940 and miR‐193a‐5p was found in HCC. Overexpression of these miRNAs suppressed HCC cell viability and invasion, promoted apoptosis and inhibited tumour growth. SPOCK1 , the common target gene of miR‐139‐5p, miR‐940 and miR‐193a‐5p, was overexpressed in HCC. SPOCK1 overexpression promoted proliferation and invasion, and restrained apoptosis of HCC cells. MiR‐139‐5p, miR‐940 and miR‐193a‐5p inhibited HCC development through targeting SPOCK1 .

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“…Previous studies have shown that miRNAs are widely dysregulated in GC tissues, 20 suggesting that miRNAs may play important roles in the development of GC. MiR‐129‐5p, the main functional executor of the miR‐129 family, is expressed at low levels in a variety of tumors and involves in cell proliferation, apoptosis, migration, and epithelial mesenchymal transition 21‐23 . It also play roles in the autophagy 24 and chemotherapy resistance 25 .…”

Section: Discussionmentioning

confidence: 99%

MiR‐129‐5p induces cell cycle arrest through modulating HOXC10/Cyclin D1 to inhibit gastric cancer progression

Lin

et al. 2020

FASEB j.

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MicroRNAs (miRNAs) play important roles in posttranscriptional regulation and may serve as targets for the diagnosis and treatment of cancers. Nevertheless, a comprehensive understanding of miRNAs profiles in gastric cancer progression is still lacking. Here, we report that miR‐129‐5p is downregulated in gastric cancer by analyzing TCGA database (n = 41) and clinical tumor samples (n = 60). MiR‐129‐5p transfection suppressed gastric cancer cell proliferation through inducing G1 phase arrest in vitro and inhibit xenograft tumor growth in vivo. MiR‐129‐5p directly targeted the 3′ untranslated regions (3′ UTR) of HOXC10 mRNA and downregulated its expression. Importantly, miR‐129‐5p could reverse the oncogenic effect induced by HOXC10. We systemically screened the downstream target of HOXC10 by ChIP sequencing, and found that HOXC10 could transcriptionally regulate the expression of Cyclin D1 and facilitate G1/S cell cycle transition. Notably, high levels of HOXC10 and Cyclin D1 were related with poor prognosis of gastric cancer patients (n = 90). These findings reveal a novel role of miR‐129‐5p/HOXC10/Cyclin D1 axis in modulating cell cycle and gastric tumorigenesis, which might provide potential prognostic biomarkers and therapeutic targets for gastric cancer patients.

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MiR-129-5p influences the progression of gastric cancer cells through interacting with SPOCK1

Cited by 23 publications

References 33 publications

MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2

MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2

MiR‐139‐5p, miR‐940 and miR‐193a‐5p inhibit the growth of hepatocellular carcinoma by targeting SPOCK1

MiR‐129‐5p induces cell cycle arrest through modulating HOXC10/Cyclin D1 to inhibit gastric cancer progression

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