MiR‐129‐5p inhibits glioma cell progression in vitro and in vivo by targeting TGIF2

Abstract

This study purposed to explore the correlation between miR‐129‐5p and TGIF2 and their impacts on glioma cell progression. Differentially expressed miRNA was screened through microarray analysis. MiR‐129‐5p expression levels in glioma tissues and cells were measured by qRT‐PCR. CCK‐8 assay, flow cytometer, transwell assay and wound‐healing assay were employed to detect cell proliferation, apoptosis and cycle, invasiveness and migration, respectively. Dual‐luciferase reporting assay was performed to confirm the targeted relationship between miR‐129‐5p and TGIF2. The effects of TGIF2 expression on cell biological functions were also investigated using the indicated methods. Tumour xenograft was applied to explore the impact of miR‐129‐5p on tumorigenesis in vivo. MiR‐129‐5p expression was down‐regulated in both glioma tissues and glioma cells, while TGIF2 expression was aberrantly higher than normal level. Dual‐luciferase reporter assay validated the targeting relation between miR‐129‐5p and TGIF2. Overexpression of miR‐129‐5p or down‐regulation of TGIF2 inhibited the proliferation, invasion and migration capacity of glioma cells U87 and U251, and meanwhile blocked the cell cycle as well as induced cell apoptosis. MiR‐129‐5p overexpression repressed the tumour development in vivo. MiR‐129‐5p and TGIF2 had opposite biological functions in glioma cells. MiR‐129‐5p could inhibit glioma cell progression by targeting TGIF2, shining light for the development of target treatment for glioma.

Polycomb complex mediated epigenetic reprogramming alters TGF‐β signaling via a novel EZH2/miR‐490/TGIF2 axis thereby inducing migration and EMT potential in glioblastomas

“…Literature so far suggests the dual nature of TGIF2 where it may function as a tumor suppressor or as an oncogene . Also, in line with our observation, TGIF2 was shown to promote aggressive phenotype in gliomas . Interestingly, some studies have reported TGF‐β signaling to have a context‐dependent role which could also be mediated via TGIF2 induced singling .…”

“…[36][37][38] Also, in line with our observation, TGIF2 was shown to promote aggressive phenotype in gliomas. 39 Interestingly, some studies have reported TGF-β signaling to have a context-dependent role which could also be mediated via TGIF2 induced singling. 40 Our TCGA data analysis showed TGIF2 to exhibit an inverse correlation with both miR-490-3p as well as CHRM2 in GBM patient samples thereby adding to its significance in gliomas.…”

PCAT‐1 promotes cell growth by sponging miR‐129 via MAP3K7/NF‐κB pathway in multiple myeloma

“…MiR‐129 is a recently discovered tumour‐associated miRNA found to be dysregulated in cancers. Diao et al demonstrated that miR‐129 combined with TGIF2 could disturb glioma progression. In the case of glioblastoma multiforme, miR‐129 blocked the ability of cell proliferation, invasion, migration, neurosphere formation, angiogenesis and temozolomide resistance via Wnt5a by blocking the PKC/ERK/NF‐κB and JNK pathways .…”

miR-541 serves as a prognostic biomarker of osteosarcoma and its regulatory effect on tumor cell proliferation, migration and invasion by targeting TGIF2

“…TGIF2 has been identified as an oncogene in some cancers, such as lung adenocarcinoma [30] and glioma [31]. In OS, TGIF2 has also been reported to be involved in tumor progression [32].…”