miR-1285-3p Controls Colorectal Cancer Proliferation and Escape from Apoptosis through DAPK2

Villanova, Lidia; Barbini, Chiara; Piccolo, Cristina; Boe, Alessandra; Maria, Ruggero De; Fiori, Micol Eleonora

doi:10.3390/ijms21072423

Cited by 14 publications

(9 citation statements)

References 44 publications

(51 reference statements)

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“…It is estimated that around 1 million people will be affected by CRC every year, accompanied by overt metastatic disease [2]. Recently, several methods including radiotherapy and chemoradiotherapy have been used for the treatment of CRC for years [5][6][7]. However, the prognosis of patients with CRC is still unsatisfactory [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, several methods including radiotherapy and chemoradiotherapy have been used for the treatment of CRC for years [5][6][7]. However, the prognosis of patients with CRC is still unsatisfactory [5][6][7]. Therefore, identifying specific biomarkers is of great importance to improve early diagnosis and investigate novel treatment strategies for patients of CRC.…”

Section: Introductionmentioning

confidence: 99%

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Exosomal miR-224-5p from Colorectal Cancer Cells Promotes Malignant Transformation of Human Normal Colon Epithelial Cells by Promoting Cell Proliferation through Downregulation of CMTM4

Yang

Shang

et al. 2022

Oxidative Medicine and Cellular Longevity

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Background. Interactions between malignant cells and neighboring normal cells are important for carcinogenesis. In addition, cancer cell-derived exosomes have been shown to promote the malignant transformation of recipient cells, but the mechanisms remain unclear. Methods. The level of miR-224-5p in CRC cell-derived exosomes was determined by RT-qPCR assay. In addition, PKH26 dye-labeled exosomes were used to assess the efficacy of the transfer of exosomes between SW620 and normal colon epithelial cell line CCD 841 CoN. Results. In this study, we found that overexpression of miR-224-5p significantly promoted the proliferation, migration, and invasion and inhibited the oxidative stress of SW620 cells. In addition, miR-224-5p can be transferred from SW620 cells to CCD 841 CoN cells via exosomes. SW620 cell-derived exosomal miR-224-5p markedly promoted proliferation, migration, and invasion of CCD 841 CoN cells. Meanwhile, SW620 cell-derived exosomal miR-224-5p notably decreased the expression of CMTM4 in CCD 841 CoN cells. Furthermore, SW620 cell-derived exosomal miR-224-5p significantly promoted tumor growth in a xenograft model in vivo. Conclusion. These findings suggested that SW620 cell-derived exosomal miR-224-5p could promote malignant transformation and tumorigenesis in vitro and in vivo via downregulation of CMTM4, suggesting that miR-224-5p might be a potential target for therapies in CRC.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exosomal miR-224-5p from Colorectal Cancer Cells Promotes Malignant Transformation of Human Normal Colon Epithelial Cells by Promoting Cell Proliferation through Downregulation of CMTM4

Yang

Shang

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…Previous reports presented that miR-1285-3p modulated multiple molecular targets and served as a sponge in ovarian cancer 29 , oral squamous cell carcinoma 30 , and cervical cancer 31 . Upregulated the expression of miR-1285-3p also inhibited cell proliferation and escape from apoptosis of colorectal cancer 32 . Our study confirmed that the expression level of circRNA_400029 was negatively correlated with miR-1285-3p in CC tissues and cells.…”

Section: Discussionmentioning

confidence: 95%

CircRNA_400029 promotes the aggressive behaviors of cervical cancer by regulation of miR-1285-3p/TLN1 axis

Ma¹,

Liu²,

Yang³

et al. 2022

J. Cancer

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Background: Cervical cancer (CC) is the highest incidence of female malignant tumor in China. Circular RNAs (circRNAs) has been reported to affect CC progression by altering mRNA stability at the transcriptional level or binding to miRNAs to produce competitive endogenous RNA (ceRNA). For this purpose, our study was aimed to investigate the function effects and the potential regulatory mechanism of the circRNA_400029 in CC cells. Materials and methods:The expression levels of circRNA_400029 and miR-1285-3p were detected by real time polymerase chain reaction (RT-PCR). Similarly, the mRNA and protein levels of TLN1 (Talin 1) was detected by RT-PCR and Western blot. Cell-Counting Kit-8 (CCK-8), EdU and Flow cytometry assay were used to detect cell proliferation, cell cycle and apoptosis. Then the Transwell assays were used to test cell migration and invasion. Besides this, the functional targets were confirmed by Dual luciferase reporter assays. Tumor xenograft in nude mice checked the result in vivo. Results: To begin with, circRNA_400029 was upregulated in CC cells and tissue. Knockdown circRNA_400029 inhibited cell proliferation, migration and invasion while induced cell apoptosis. Interestingly, miR-1285-3p targeted circRNA_400029 and down-regulated of miR-1285-3p could reverse the effects of circRNA_400029 weak-expression on progression and apoptosis of CC cells. Moreover, TLN1 was up-regulated in CC cells and identified as a direct target of miR-1285-3p. Meanwhile, we found that miR-1285-3p negatively regulated the function of TLN1.Finally, the circRNA_ 400029/miR-1285-3p/TLN1 axis could affect tumor growth in vivo. Conclusion:The overexpressed circRNA_400029 promoted CC proliferation, migration and invasion while deduced apoptosis by sponging miR-1285-3p to regulate TLN1. CircRNA_400029 was a potential onco-circRNA in CC, and might be a promised therapy target.

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“…Moreover, miR-135a promoted the proliferation and invasion of oxaliplatin-resistant gastric cancer cells, and inhibited E2F transcription factor 1 (E2F1) induced apoptosis by inhibiting E2F1 and DAPK2 expression (Yan et al, 2016b). In colorectal cancer, miR-1285-3p improved colorectal cancer cell proliferation and escape from apoptosis by targeting DAPK2 (Villanova et al, 2020). In diabetic cardiomyopathy (DCM), lncRNA myocardial infarction associated transcript (MIAT) was significantly upregulated in the rat model of DCM.…”

Section: Non-coding Rnas Involved In the Transcription Regulation Of Death-associated Protein Kinase2mentioning

confidence: 99%

Regulatory Non-coding RNAs for Death Associated Protein Kinase Family

Wang

Lin

Zhong

et al. 2021

Front. Mol. Biosci.

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The death associated protein kinases (DAPKs) are a family of calcium dependent serine/threonine kinases initially identified in the regulation of apoptosis. Previous studies showed that DAPK family members, including DAPK1, DAPK2 and DAPK3 play a crucial regulatory role in malignant tumor development, in terms of cell apoptosis, proliferation, invasion and metastasis. Accumulating evidence has demonstrated that non-coding RNAs, including microRNA (miRNA), long non-coding RNA (lncRNA) and circRNA, are involved in the regulation of gene expression and tumorigenesis. Recent studies indicated that non-coding RNAs participate in the regulation of DAPKs. In this review, we summarized the current knowledge of non-coding RNAs, as well as the potential miRNAs, lncRNAs and circRNAs, that are involved in the regulation of DAPKs.

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miR-1285-3p Controls Colorectal Cancer Proliferation and Escape from Apoptosis through DAPK2

Cited by 14 publications

References 44 publications

Exosomal miR-224-5p from Colorectal Cancer Cells Promotes Malignant Transformation of Human Normal Colon Epithelial Cells by Promoting Cell Proliferation through Downregulation of CMTM4

Exosomal miR-224-5p from Colorectal Cancer Cells Promotes Malignant Transformation of Human Normal Colon Epithelial Cells by Promoting Cell Proliferation through Downregulation of CMTM4

CircRNA_400029 promotes the aggressive behaviors of cervical cancer by regulation of miR-1285-3p/TLN1 axis

Regulatory Non-coding RNAs for Death Associated Protein Kinase Family

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