CircRNA_400029 promotes the aggressive behaviors of cervical cancer by regulation of miR-1285-3p/TLN1 axis

Abstract

Background: Cervical cancer (CC) is the highest incidence of female malignant tumor in China. Circular RNAs (circRNAs) has been reported to affect CC progression by altering mRNA stability at the transcriptional level or binding to miRNAs to produce competitive endogenous RNA (ceRNA). For this purpose, our study was aimed to investigate the function effects and the potential regulatory mechanism of the circRNA_400029 in CC cells. Materials and methods:The expression levels of circRNA_400029 and miR-1285-3p were detected by real time polymerase chain reaction (RT-PCR). Similarly, the mRNA and protein levels of TLN1 (Talin 1) was detected by RT-PCR and Western blot. Cell-Counting Kit-8 (CCK-8), EdU and Flow cytometry assay were used to detect cell proliferation, cell cycle and apoptosis. Then the Transwell assays were used to test cell migration and invasion. Besides this, the functional targets were confirmed by Dual luciferase reporter assays. Tumor xenograft in nude mice checked the result in vivo. Results: To begin with, circRNA_400029 was upregulated in CC cells and tissue. Knockdown circRNA_400029 inhibited cell proliferation, migration and invasion while induced cell apoptosis. Interestingly, miR-1285-3p targeted circRNA_400029 and down-regulated of miR-1285-3p could reverse the effects of circRNA_400029 weak-expression on progression and apoptosis of CC cells. Moreover, TLN1 was up-regulated in CC cells and identified as a direct target of miR-1285-3p. Meanwhile, we found that miR-1285-3p negatively regulated the function of TLN1.Finally, the circRNA_ 400029/miR-1285-3p/TLN1 axis could affect tumor growth in vivo. Conclusion:The overexpressed circRNA_400029 promoted CC proliferation, migration and invasion while deduced apoptosis by sponging miR-1285-3p to regulate TLN1. CircRNA_400029 was a potential onco-circRNA in CC, and might be a promised therapy target.

Cervical extracellular matrix hydrogel optimizes tumor heterogeneity of cervical squamous cell carcinoma organoids

“…ITGB1, a crucial integrin subunit, is essential for mediating cell-ECM interactions, thereby contributing to the formation of focal adhesions ( 71 ). Furthermore, various other proteins present in EVs, such as ANXA2 ( 72 , 73 ), TLN1 ( 74 ), ENO1 ( 75 ), and TFRC ( 76 ) exhibit substantial overexpression in cervical cancer. These proteins play a role in cell adhesion and movement, regulating tumor cell proliferation and metastasis, and are also linked to patient prognosis.…”

Exosome‐derived circTFDP2 promotes prostate cancer progression by preventing PARP1 from caspase‐3‐dependent cleavage

“…For example, circRNA_400029 promoted cervical cancer progression by sponging miR‐1285‐3p, finally regulating TLN1 expression. 35 Moreover, circRNAs can interact with RBPs. Zhang et al.…”

“…For example, circRNA_400029 promoted cervical cancer progression by sponging miR-1285-3p, finally regulating TLN1 expression. 35 Moreover, circRNAs can interact with RBPs. Zhang et al 11 showed that circVPS13C could bind with RRBP1 proteins to decrease the stability of IFITM1 mRNA, eventually promoting pituitary adenoma progression.…”

“…The most well‐studied mechanism of circRNAs is their function as miRNA sponges. For example, circRNA_400029 promoted cervical cancer progression by sponging miR‐1285‐3p, finally regulating TLN1 expression 35 . Moreover, circRNAs can interact with RBPs.…”

EREG, HOPX and SYNGR3 influence the Pathogenesis and Prognosis of Cervical Cancer through immune cells infiltration

“…The competitive endogenous RNA (ceRNA) theory, which proposes that non-coding RNA, mRNA, and other transcripts can competitively bind miRNA response elements (MREs) to mutually regulate the expression of their respective genes, is central to the interaction mechanism between RNAs [6]. A large number of studies have discovered that aberrant circRNA regulation causes a variety of diseases, including cervical cancer [7][8][9]. Through bioinformatics research and multiple databases, such as the Gene Expression Omnibus (GEO) database, the cancerspeci c circRNA database (CSCD), and the TargetScan database, we attempt to create the circRNA-miRNA-mRNA-related ceRNA regulation network for cervical cancer.…”