MiR-125b promotes migration and invasion by targeting the vitamin D receptor in renal cell carcinoma

Abstract

Purpose: To investigate the expression of miR-125b and vitamin D receptor (VDR) in renal cell carcinoma (RCC) and assess the biological function of miR-125b in RCC. Methods: We used quantitative real-time polymerase chain reaction (RT-PCR) to detect the expression of nucleic acids and western blotting to analyze the protein abundance in RCC cell lines. MiR-125b mimic and inhibitor were employed to investigate the function and behavior of miR-125b in RCC cell lines. The relationship between miR-125 and VDR was verified using luciferase assays. Results: Overexpression of miR-125b promoted migration and invasion and prevent cell apoptosis in ACHN cells. In contrast, miR-125b deficiency suppressed migration and invasion and induced cell apoptosis in 786-O cells. Luciferase assays indicated the interaction between miR-125b and VDR. In collected samples, miR-125b was significantly higher in RCC tissues and negatively correlated to VDR (r=-0.444, p=0.04). Conclusion: MiR-125b displays an oncogene profile in RCC, patients with high expression of miR-125b should be a more frequent follow-up. MiR-125B may be a potential therapeutic target for RCC.

Ten-Year Recurrence Outcomes in Early Stage and Early Onset Colon Cancer and the Expression Profiles of MicroRNAs and Cancer Stem Cell Markers in These Tumors

“…miR-125a was significantly downregulated in breast and gastric cancer, and miR-125a substantially inhibited cancer cells' proliferation, migration, and invasion activities. [27][28][29] There is only one study showing an association between miR-125b and CSCs. Zhoug et al determined that miR-125b negatively correlated with CD133 expression in hepatocellular carcinoma patients.…”

miR‑5590‑3p inhibits the proliferation and metastasis of renal cancer cells by targeting ROCK2 to inhibit proliferation, migration and invasion

“…Çaykara et al ( 27 ) indicated that abnormally downregulated miR-124 was associated with the tumor stage, tumor size and neutrophil count in patients with RCC, and restoration of miR-124 expression may be effective in the treatment of RCC. He et al ( 28 ) reported that miR-125b was significantly upregulated in RCC tissues, which was negatively correlated with vitamin D receptor (VDR) levels. Through mediating VDR, miR-125 is capable of enhancing proliferative and migratory rates of RCC cells, suggesting miR-125 may be a potential therapeutic target of RCC.…”