miR‑5590‑3p inhibits the proliferation and metastasis of renal cancer cells by targeting ROCK2 to inhibit proliferation, migration and invasion

Liu, Queling; Zhu, Anyi; Gao, Weiyin; Gui, Fu; Zou, Yan; Zhou, Xiaocheng; Zhou, Hong

doi:10.3892/ol.2022.13497

Cited by 4 publications

(3 citation statements)

References 33 publications

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“…Similarly, it is reported that miR‐5590‐3p is downregulated in triple‐negative breast cancer (TNBC), and elevation of miR‐5590‐3p suppresses TNBC cell proliferation and migration, as well as boosts cell apoptosis. 19 Liu et al 42 have supported that miR‐5590‐3p is downregulated in renal cell carcinoma (RCC), and transfection of miR‐5590‐3p mimic hinders RCC cell proliferative, migratory, and invasive capacities. Furthermore, we also found that miR‐5590‐3p targeted and negatively regulated DCBLD2 expression.…”

Section: Discussionmentioning

confidence: 99%

LncRNA MIR100HG affects the proliferation and metastasis of lung cancer cells through mediating the microRNA‐5590‐3p/DCBLD2 axis

Min,

Zhang,

Zhang

et al. 2024

Immunity Inflam & Disease

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ObjectiveThe aim of this paper is to investigate the effect of long noncoding RNA (lncRNA) MIR100HG on the proliferation and metastasis of lung cancer cells by mediating the microRNA (miR)−5590‐3p/DCBLD2 axis.MethodsRNA levels of MIR100HG, miR‐5590‐3p, and DCBLD2 in lung cancer tissues and cells were detected by quantitative reverse‐transcription polymerase chain reaction, and protein level was assessed by Western blot. Effects of MIR100HG or miR‐5590‐3p on proliferation, migration, and invasion of lung cancer cells were detected by Cell Counting Kit‐8, colony formation, and Transwell assays. Luciferase reporter assay and RNA‐immunoprecipitation assay confirmed the target relationship between miR‐5590‐3p and MIR100HG or DCBLD2.ResultsMIR100HG and DCBLD2 were highly expressed, while miR‐5590‐3p was lowly expressed in lung cancer tissues and cells. Silencing MIR100HG or upregulating miR‐5590‐3p impeded lung cancer cell proliferation, migration, and invasion. MIR100HG could up‐regulate DCBLD2 by sponging miR‐5590‐3p. Downregulation of miR‐5590‐3p partly overturned the suppressive effect of silencing MIR100HG on lung cancer cell proliferation and metastasis, and overexpression of DCBLD2 also reversed the effect of overexpression of miR‐5590‐3p on lung cancer cell proliferation and metastasis.ConclusionLncRNA MIR100HG promotes lung cancer progression by targeting and negatively regulating DCBLD2 through binding with miR‐5590‐3p.

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Section: Discussionmentioning

confidence: 99%

LncRNA MIR100HG affects the proliferation and metastasis of lung cancer cells through mediating the microRNA‐5590‐3p/DCBLD2 axis

Min,

Zhang,

Zhang

et al. 2024

Immunity Inflam & Disease

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“…Involved in many cellular processes, miRNAs are important EV cargoes. In fact, the differential expression levels of miRNAs have been associated with renal damage; they have therefore been used as biomarkers of the disease and/or proposed as potential therapeutic targets [35,[46][47][48]. Taking this into account, we sought to determine their levels in cellular pellets and EVs from podocytes silenced and treated with glucose.…”

Section: Discussionmentioning

confidence: 99%

Rab3A/Rab27A System Silencing Ameliorates High Glucose-Induced Injury in Podocytes

Martínez-Arroyo

Flores-Chova

Sanchez-Garcia

et al. 2023

Biology

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Diabetic nephropathy is a major complication in diabetic patients. Podocytes undergo loss and detachment from the basal membrane. Intra- and intercellular communication through exosomes are key processes for maintaining function, and the Rab3A/Rab27A system is an important counterpart. Previously, we observed significant changes in the Rab3A/Rab27A system in podocytes under glucose overload, demonstrating its important role in podocyte injury. We investigated the implication of silencing the Rab3A/Rab27A system in high glucose-treated podocytes and analysed the effect on differentiation, apoptosis, cytoskeletal organisation, vesicle distribution, and microRNA expression in cells and exosomes. For this, we subjected podocytes to high glucose and transfection through siRNAs, and we isolated extracellular vesicles and performed western blotting, transmission electron microscopy, RT-qPCR, immunofluorescence and flow cytometry assays. We found that silencing RAB3A and RAB27A generally leads to a decrease in podocyte differentiation and cytoskeleton organization and an increase in apoptosis. Moreover, CD63-positive vesicles experienced a pattern distribution change. Under high glucose, Rab3A/Rab27A silencing ameliorates some of these detrimental processes, suggesting a differential influence depending on the presence or absence of cellular stress. We also observed substantial expression changes in miRNAs that were relevant in diabetic nephropathy upon silencing and glucose treatment. Our findings highlight the Rab3A/Rab27A system as a key participant in podocyte injury and vesicular traffic regulation in diabetic nephropathy.

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“…Studies had shown that miR-5590-3p downregulation facilitated proliferation in prostate cancer cells [11] . Besides, miR-5590-3p had been confirmed to restrain cell invasion in ovarian cancer and renal cancer [12,13] . Therefore, miR-5590-3p may serve as a tumor suppressor.…”

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confidence: 99%

Sapindus Saponin Inhibits Colorectal Cancer cell Proliferation and Metastasis by regulating microRNA-5590-3p expression

Zhang,

Wang,

Cao

2023

IJPS

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Zhang et al.: Sapindus Saponin in Colorectal Cancer Cells and Its MechanismThis study explored Sapindus saponins role in the proliferation and metastasis of colorectal cancer cells and its possible mechanism. SW620 cells were divided into 8 groups; control group, Sapindus saponin-low group, Sapindus saponin-middle group, Sapindus saponin-high group, microRNA+negative control group, microRNA-5590-3p group, Sapindus saponin+anti-microRNA-negative control group and Sapindus saponin+anti-microRNA-5590-3p group. Cell proliferation and metastasis were determined using colony formation assay, wound healing assay, 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay and transwell assay. MicroRNA-5590-3p expression was detected by quantitative reverse transcription-polymerase chain reaction. Matrix metalloproteinases-2 and matrix metalloproteinases-9 protein levels were examined by Western blot. SW620 cell proliferation inhibition rate and microRNA-5590-3p expression were increased, while colony numbers, invaded cell numbers, wound healing rate, matrix metalloproteinases-2 and matrix metalloproteinases-9 protein levels were decreased in Sapindus saponin-low group, Sapindus saponin-middle group, Sapindus saponin-high group in a dose-dependent manner. Besides, microRNA-5590-3p overexpression increased SW620 cell proliferation inhibition rate, while suppressed colony numbers, invaded cell numbers, wound healing rate, matrix metalloproteinases-2 and matrix metalloproteinases-9 protein levels. Compared with the Sapindus saponin+anti-microRNA-negative control group, SW620 cell proliferation inhibition rate was reduced, while colony numbers, invaded cell numbers, wound healing rate, matrix metalloproteinases-2 and matrix metalloproteinases-9 protein levels were enhanced in the Sapindus saponin+anti-microRNA-5590-3p group. Sapindus saponin could repress colorectal cancer cell proliferation and metastasis by upregulating microRNA-5590-3p.

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miR‑5590‑3p inhibits the proliferation and metastasis of renal cancer cells by targeting ROCK2 to inhibit proliferation, migration and invasion

Cited by 4 publications

References 33 publications

LncRNA MIR100HG affects the proliferation and metastasis of lung cancer cells through mediating the microRNA‐5590‐3p/DCBLD2 axis

LncRNA MIR100HG affects the proliferation and metastasis of lung cancer cells through mediating the microRNA‐5590‐3p/DCBLD2 axis

Rab3A/Rab27A System Silencing Ameliorates High Glucose-Induced Injury in Podocytes

Sapindus Saponin Inhibits Colorectal Cancer cell Proliferation and Metastasis by regulating microRNA-5590-3p expression

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