MiR-125a-5p silencing inhibits cerebral ischemia-induced injury
through targeting IGFBP3

Song, Bo; Xu, Jing; Zhong, Ping; Fang, Lei

doi:10.5114/fn.2021.107109

Cited by 8 publications

(2 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The insulin-like growth factor binding protein (IGFBP) family consists of peptides that share similar structures, each approximately 260 amino acids long [22]. These peptides display different binding sites for miRNAs and are crucial in cell apoptosis and angiogenesis [23,24]. In addition, IGFBP can combine with IGF to influence cell proliferation, migration, differentiation, angiogenesis and apoptosis [25].…”

Section: Introductionmentioning

confidence: 99%

Testosterone promotes human foreskin fibroblast growth through miR-143-3p targeting IGFBP-3

2023

Journal of Men's Health

View full text Add to dashboard Cite

Testosterone is an important male hormone, which could improve the maintenance and recovery of gonadal function in males as well as the repair of human hypospadias and cell fibrosis. Our study focused on investigating the regulatory effect of testosterone in human foreskin fibroblasts (HFF-1) and regulatory mechanisms involved. In this study, HFF-1 cells were treated with testosterone, and cell viability and migration were assessed by cell counting kit-8 (CCK8) and Transwell assays. The expression levels of androgen receptor (AR), miR-143-3p and insulin-like growth factor binding protein-3 (IGFBP-3) were measured by quantitative real-time PCR (qRT-PCR), Western blotting, and immunofluorescence. In addition, a potential binding site for miR-143-3p on IGFBP-3 was predicted and its direct binding was further confirmed by a dual luciferase reporter assay. These results showed that testosterone increased the viability and migration of HFF-1 cells. Testosterone could down-regulate miR-143-3p and up-regulate IGFBP-3 and AR. Overexpression of miR-143-3p hindered HFF-1 cell viability and negatively regulated IGFBP-3, whereas inhibition of IGFBP-3 impeded cell viability and migration. Furthermore, miR-143-3p was found to directly bind to IGFBP-3. Overexpression of IGFBP-3 countered the regulation of HFF-1 cells by miR-143-3p mimics. In conclusion, this study showed that testosterone promoted the proliferation and migration of HFF-1 cells and AR signaling, at least via the miR-143-3p/IGFBP-3 axis. This discovery presents a novel insight for testosterone application in male disorders like hypospadias.

show abstract

Section: Introductionmentioning

confidence: 99%

Testosterone promotes human foreskin fibroblast growth through miR-143-3p targeting IGFBP-3

2023

Journal of Men's Health

View full text Add to dashboard Cite

show abstract

“…MicroRNAs (miRNAs) are endogenous small non‐coding RNAs that are important in regulating gene expression by binding to the 3′ UTR of target mRNA during post‐transcriptional processing steps (Wang et al, 2014). Abnormally expressed miRNAs are important regulators of cerebrovascular diseases, including ischemic stroke (Song et al, 2021). miR‐21‐5p is an anti‐apoptotic miRNA that has a protective effect on oxidative stress‐induced cell death (Li, Wei, et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Effect of remote limb ischemic post‐conditioning on the expression of miR‐21‐5p/PirB in the brain of rats with focal cerebral ischemia

Jiao

Wang

Xue

2022

Eur J of Neuroscience

View full text Add to dashboard Cite

Cerebral ischemia causes great damage to ischemic brain regions. Remote limb post-conditioning (RLIPostC) has neuroprotective effects on cerebral ischemia.Paired immunoglobulin receptor B (PirB) has been confirmed to affect the cerebral ischemia injury. Therefore, this study investigated the mechanism of RLIPostC on cerebral ischemia injury. The middle cerebral artery occlusion (MCAO) model was established in rats, followed by RLIPostC (by blocking the blood flow in the distal limb for 10 min after MCAO, and restored for 10 min, three cycles), or in vivo lentivirus infection of miR-21-5p agomir, sh-PirB and oe-PirB. The locomotor abilities were assessed using the foot fault test and balance beam walking test at 48 h, 7 and 21 days after operation, and neurological function was measured on day 21. The cerebral infarct area, Nissl bodies and the pathology of brain tissues were examined using histological staining, followed by the assessment of miR-21-5p and PirB levels and the binding relationship between miR-21-5p and PirB. miR-21-5p was poorly expressed in MCAO rats. Both RLIPostC and overexpression of miR-21-5p reduced motor dysfunction, neurological function score, cerebral infarction area and brain edema; increased Nissl bodies in MCAO rats and thus alleviated the brain damage caused by cerebral ischemia. RLIPostC promoted miR-21-5p expression. PirB was highly expressed in cerebral ischemia, and miR-21-5p targeted PirB. Overall, RLIPostC promotes miR-21-5p expression and then inhibits PirB to alleviate cerebral ischemia injury, which provides theoretical basis for basic research on motor dysfunction and neuron injury after cerebral ischemia and the development of neuroscience.

show abstract

MiR-203a-3p/153-3p improves cognitive impairments induced by ischemia/reperfusion via blockade of SRC-mediated MAPK signaling pathway in ischemic stroke

Peng

et al. 2022

Chemico-Biological Interactions

View full text Add to dashboard Cite

MiR-125a-5p silencing inhibits cerebral ischemia-induced injury through targeting IGFBP3

Cited by 8 publications

References 27 publications

Testosterone promotes human foreskin fibroblast growth through miR-143-3p targeting IGFBP-3

Testosterone promotes human foreskin fibroblast growth through miR-143-3p targeting IGFBP-3

Effect of remote limb ischemic post‐conditioning on the expression of miR‐21‐5p/PirB in the brain of rats with focal cerebral ischemia

MiR-203a-3p/153-3p improves cognitive impairments induced by ischemia/reperfusion via blockade of SRC-mediated MAPK signaling pathway in ischemic stroke

Contact Info

Product

Resources

About