MIP-based electrochemical sensor for direct detection of hepatitis C virus via E2 envelope protein

Antipchik, Mariia; Reut, Jekaterina; Ayankojo, Akinrinade George; Öpik, Andres; Syritski, Vitali

doi:10.1016/j.talanta.2022.123737

Cited by 18 publications

(6 citation statements)

References 60 publications

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“…Antipchik et al [ 123 ] published the first report on the development of an MIP-based electrochemical sensor for detecting the hepatitis C virus via its surface protein E2. Green fluorescent protein was used in conjunction with E2 (total MW of 54 kDa) to prevent protein agglomeration and stabilize its structure.…”

Section: Mip Application For the Detection Of Biomarkers Of Infectiou...mentioning

confidence: 99%

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Molecularly Imprinted Polymer-Based Electrochemical Sensors for the Diagnosis of Infectious Diseases

et al. 2023

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The appearance of biological molecules, so-called biomarkers in body fluids at abnormal concentrations, is considered a good tool for detecting disease. Biomarkers are usually looked for in the most common body fluids, such as blood, nasopharyngeal fluids, urine, tears, sweat, etc. Even with significant advances in diagnostic technology, many patients with suspected infections receive empiric antimicrobial therapy rather than appropriate treatment, which is driven by rapid identification of the infectious agent, leading to increased antimicrobial resistance. To positively impact healthcare, new tests are needed that are pathogen-specific, easy to use, and produce results quickly. Molecularly imprinted polymer (MIP)-based biosensors can achieve these general goals and have enormous potential for disease detection. This article aimed to overview recent articles dedicated to electrochemical sensors modified with MIP to detect protein-based biomarkers of certain infectious diseases in human beings, particularly the biomarkers of infectious diseases, such as HIV-1, COVID-19, Dengue virus, and others. Some biomarkers, such as C-reactive protein (CRP) found in blood tests, are not specific for a particular disease but are used to identify any inflammation process in the body and are also under consideration in this review. Other biomarkers are specific to a particular disease, e.g., SARS-CoV-2-S spike glycoprotein. This article analyzes the development of electrochemical sensors using molecular imprinting technology and the used materials’ influence. The research methods, the application of different electrodes, the influence of the polymers, and the established detection limits are reviewed and compared.

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Section: Mip Application For the Detection Of Biomarkers Of Infectiou...mentioning

confidence: 99%

“… Schematic representation of HCV diagnostics principle by HCV sensor. Reprinted with permission from [ 123 ]. …”

Section: Figurementioning

confidence: 99%

Molecularly Imprinted Polymer-Based Electrochemical Sensors for the Diagnosis of Infectious Diseases

et al. 2023

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“…Several studies reported in the literature have successfully demonstrated the use of MIPs for the quantification of large molecules such as proteins and drugs [ 31 , 32 , 33 ] since these analytes could enter into the cavities of the imprinted polymer. However, it is worth noting that sensors based on only MIP generally do not have as suitable conductivity [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Biomimetic Material for Quantification of Methotrexate Using Sensor Based on Molecularly Imprinted Polypyrrole Film and MWCNT/GCE

et al. 2023

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This study investigates biomimetic sensors for the detection of methotrexate contaminants in environmental samples. Sensors inspired by biological systems are the focus of this biomimetic strategy. Methotrexate is an antimetabolite that is widely used for the treatment of cancer and autoimmune diseases. Due to the widespread use of methotrexate and its rampant disposal into the environment, the residues of this drug are regarded as an emerging contaminant of huge concern, considering that exposure to the contaminant has been found to lead to the inhibition of some essential metabolic processes, posing serious risks to humans and other living beings. In this context, this work aims to quantify methotrexate through the application of a highly efficient biomimetic electrochemical sensor constructed using polypyrrole−based molecularly imprinted polymer (MIP) electrodeposited by cyclic voltammetry on a glassy carbon electrode (GCE) modified with multi−walled carbon nanotubes (MWCNT). The electrodeposited polymeric films were characterized by infrared spectrometry (FTIR), scanning electron microscopy (SEM), and cyclic voltammetry (CV). The analyses conducted using differential pulse voltammetry (DPV) yielded a detection limit of 2.7 × 10−9 mol L−1 for methotrexate, a linear range of 0.01–125 μmol L−1, and a sensitivity of 0.152 μA L mol−1. The results obtained from the analysis of the selectivity of the proposed sensor through the incorporation of interferents in the standard solution pointed to an electrochemical signal decay of only 15.4%. The findings of this study show that the proposed sensor is highly promising and suitable for use in the quantification of methotrexate in environmental samples.

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“…Accordingly, molecular imprinting has become one of the most important techniques for fabricating synthetic ligands on sensor surfaces [17,19]. MIPs have been successfully coupled to surface plasmon resonance (SPR) sensors [23], quartz crystal microbalance sensors [24], luminescence probes [25] and electrochemical sensors [26,27]. Molecular-imprinted fluorescent sensors (MIFS) have been used for detection of several organic molecules and metal ions including proteins [28], caffeine [29] and cocaine [30] in addition to silver [31] and aluminum ions [32].…”

Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Solid Phase Extraction Strategy for Quinic Acid

et al. 2022

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Quinic acid (QA) and its ester conjugates have been subjected to in-depth scientific investigations for their antioxidant properties. In this study, molecularly imprinted polymers (MIPs) were used for selective extraction of quinic acid (QA) from coffee bean extract. Computational modelling was performed to optimize the process of MIP preparation. Three different functional monomers (allylamine, methacrylic acid (MAA) and 4-vinylpyridine (4-VP)) were tested for imprinting. The ratio of each monomer to template chosen was based on the optimum ratio obtained from computational studies. Equilibrium rebinding studies were conducted and MIP C, which was prepared using 4-VP as functional monomer with template to monomer ratio of 1:5, showed better binding performance than the other prepared MIPs. Accordingly, MIP C was chosen to be applied for selective separation of QA using solid-phase extraction. The selectivity of MIP C towards QA was tested versus its analogues found in coffee (caffeic acid and chlorogenic acid). Molecularly imprinted solid-phase extraction (MISPE) using MIP C as sorbent was then applied for selective extraction of QA from aqueous coffee extract. The applied MISPE was able to retrieve 81.918 ± 3.027% of QA with a significant reduction in the amount of other components in the extract.

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MIP-based electrochemical sensor for direct detection of hepatitis C virus via E2 envelope protein

Cited by 18 publications

References 60 publications

Molecularly Imprinted Polymer-Based Electrochemical Sensors for the Diagnosis of Infectious Diseases

Molecularly Imprinted Polymer-Based Electrochemical Sensors for the Diagnosis of Infectious Diseases

Biomimetic Material for Quantification of Methotrexate Using Sensor Based on Molecularly Imprinted Polypyrrole Film and MWCNT/GCE

Molecularly Imprinted Solid Phase Extraction Strategy for Quinic Acid

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