No abstract

No abstract

No abstract

No abstract

The synergistic effect of combining molecular imprinting and surface acoustic wave (SAW) technologies for the selective and label-free detection of sulfamethizole as a model antibiotic in aqueous environment was demonstrated. A molecularly imprinted polymer (MIP) for sulfamethizole (SMZ) selective recognition was prepared in the form of a homogeneous thin film on the sensing surfaces of SAW chip by oxidative electropolymerization of m-phenylenediamine (mPD) in the presence of SMZ, acting as a template. Special attention was paid to the rational selection of the functional monomer using computational and spectroscopic approaches. SMZ template incorporation and its subsequent release from the polymer was supported by IR microscopic measurements. Precise control of the thicknesses of the SMZ-MIP and respective nonimprinted reference films (NIP) was achieved by correlating the electrical charge dosage during electrodeposition with spectroscopic ellipsometry measurements in order to ensure accurate interpretation of label-free responses originating from the MIP modified sensor. The fabricated SMZ-MIP films were characterized in terms of their binding affinity and selectivity toward the target by analyzing the binding kinetics recorded using the SAW system. The SMZ-MIPs had SMZ binding capacity approximately more than eight times higher than the respective NIP and were able to discriminate among structurally similar molecules, i.e., sulfanilamide and sulfadimethoxine. The presented approach for the facile integration of a sulfonamide antibiotic-sensing layer with SAW technology allowed observing the real-time binding events of the target molecule at nanomolar concentration levels and could be potentially suitable for cost-effective fabrication of a multianalyte chemosensor for analysis of hazardous pollutants in an aqueous environment.

No abstract

The present study examines the potential hepatoprotective action of methanolic extract of Cochlospermum tinctorium leaf on carbon tetrachloride-induced liver damaged in rats. Forty rats divided into four groups (group 1-control; group 2-CCl 4 induced; group 3-CCl 4 + C. tinctorium extract (200 mg/kg); group 4-CCl 4 + prednisolone (standard anti-inflammatory drug) of ten rats in each were used. Administration of CCl 4 induced hepatic damage in rats, as evidenced by a significant (p<0.05) increase in the levels of serum glutamate pyruvate transaminase SGPT, serum glutamate oxaloacetate transaminase SGOT, cholesterol and bilirubin levels. The level of malondialdehyde MDA (an index of oxidative stress) was also higher in the CCl 4 group, when compared to other groups. However, administration of methanolic extract of C. tinctorium (200 mg/kg) after CCl 4 administration (group 3) brought a significant (p<0.05) reduction in values of these parameters compared to the CCl 4 treated group (group 1). The potential hepatoprotective activity of the extract was also demonstrated by its regenerative action on some damaged liver tissues, as evidenced by the histopathological studies of the representative liver sample of group 3 rats' liver section, which showed hepatic regeneration, with no visible pathology. This study therefore showed the potential of the C. tinctorium leaf extract to decrease the levels of serum markers enzymes, indicating the protection of hepatic cells, and to confer some levels of protection against CCl 4 induced hepatocellular injury.

No abstract