Mink-mouse hybridomas that secrete mink immunoglobulin G

Galakhar, N.L.; Djatchenko, S.N.; Fomicheva, I. I.; Mechetina, Ludmila V.; Taranin, Alexander V.; Belousov, E. S.; Nayakshin, A. M.; Baranov, O. K.

doi:10.1016/0022-1759(88)90307-9

Cited by 6 publications

(8 citation statements)

References 9 publications

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“…Davidson et al (81) compared murine myelomas as fusion partners for cattle and found that NS1 gave better results than X63. Yarmush et al (38) and Galakhar et al (62) also compared the performance of murine myelomas in rabbit and mink fusions, respectively. Both reported that the SP2 lines gave the worst results, with X63 and NS0 being better.…”

Section: Heteromyelomas-rationale and Experiencesmentioning

confidence: 96%

“…Galakahar et al (62) found feeders from nu/nu mice to be superior to those from Balb/c mice. Butcher (80) reported the use of a mitom ycin-C-inhibited 3T3/B embryonic fibroblast cell line.…”

Section: Cell Culture-feeder Cellsmentioning

confidence: 97%

“…(44) MAbs have been successfully derived from all of the common Western domestic species, including cattle, (45)(46)(47)(48) sheep, (20,49,50) goats, (51,52) pigs, (53)(54)(55) and horses (56,57) as well as cats, (58,59) dogs, (60,61) and mink. (62,63) These studies have been extensions of ongoing work on polyclonal antibodies or investigations into particular applications requiring nonmurine antibody-secreting lines. Pig (54) and sheep MAbs (23) have both been suggested as candidates for human immunotherapy.…”

Section: Other Species As Sources Of Mabsmentioning

confidence: 98%

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Review: Veterinary Sources of Nonrodent Monoclonal Antibodies: Interspecific and Intraspecific Hybridomas

Groves¹,

Morris

2000

Hybridoma

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The generation of monoclonal antibodies from species other than rats and mice has developed slowly over the last 20 years. The advent of antibody engineering and realization of the advantages of nonmurine antibodies, in terms of their superior affinities and specificities, and their potential as components of human and veterinary therapeutics has increased their relevance recently. There have been significant advances in the development of myeloma and heteromyeloma fusion partners. This is an opportune moment to consolidate experiences of MAb production across the range of species of veterinary interest and place it into context with other developments in the field of monoclonal antibodies. The background to the development of antibodies from species other than the mouse is discussed. The species and antigens used to date are reviewed, as are the methods and results reported. A suggested protocol is provided for first attempts to exploit the huge potential of this aspect of hybridoma technology and suggestions are made for its further expansion.

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Section: Heteromyelomas-rationale and Experiencesmentioning

confidence: 96%

Section: Cell Culture-feeder Cellsmentioning

confidence: 97%

Section: Other Species As Sources Of Mabsmentioning

confidence: 98%

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Review: Veterinary Sources of Nonrodent Monoclonal Antibodies: Interspecific and Intraspecific Hybridomas

Groves¹,

Morris

2000

Hybridoma

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“…wt. 1500, "Serva", GFR) (6). Cell suspensions in RPMI 1640 medium with 10% fetal calf serum, 2 mM L-glutamine, 1 mM sodium pyruvate, 20 mM HEPES, 50 ug/ml gentamycin and 1x10"4 hypoxanthine, 4x10"7 aminopterin, 1.6x10"5 M thymidine (HAT) were poured into 192 wells of 96-well tissue culture plates (Titertek, Flow Labs).…”

Section: Fusionmentioning

confidence: 99%

“…During the first cloning, the cells were seeded in HAT medium; during the following ones, without HAT components. Spleen cells of BALB/c nu/nu mice were used as feeder layers (6). Cloning efficiency n was calculated from a formula: n= (number of hybridoma clones x 100% (total number of seeded wells) x (seeding frequency)…”

Section: Cloningmentioning

confidence: 99%

Mink-Mouse Interspecific Hybridomas

Ufimtseva

Galakhar²,

Matjakhina³

et al. 1991

Hybridoma

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Mink-mouse interspecific hybridomas were produced by fusion of the american mink spleen cells with the NSO cells. Seven cloned lines of the mink-mouse hybridoma were isolated, and their functional mink Ig secretion and karyological characteristics are given. During cytogenetic analysis of mink-mouse hybridoma cell lines, we observed the elimination of mink chromosomes, and inter- and intralineral variability of the numbers of the cells with particular quantities of mink DNA. We did not find that the characteristic peculiarities of mink DNA distribution in the hybridoma cell lines had any bearing upon the secretion or non-secretion of mink Ig. There was no synthesis of lambda-L-chains of mink Ig in line 7 cells because the line lost the lambda-gene. With the aid of in situ hybridization with 3H-labeled total mink DNA, a considerable transformation of hybridoma cell karyotype was observed. Multiple integration of the mink DNA into mouse chromosomes and the appearance of chromosomes not characteristic for either the mink or mouse parent cells were noted. Increasing numbers of cells with translocations of mink chromosomes fragments into mouse chromosomes were found in the hybridoma lines cultivated for lengthy periods.

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