Mink-Mouse Interspecific Hybridomas

Abstract

Mink-mouse interspecific hybridomas were produced by fusion of the american mink spleen cells with the NSO cells. Seven cloned lines of the mink-mouse hybridoma were isolated, and their functional mink Ig secretion and karyological characteristics are given. During cytogenetic analysis of mink-mouse hybridoma cell lines, we observed the elimination of mink chromosomes, and inter- and intralineral variability of the numbers of the cells with particular quantities of mink DNA. We did not find that the characteristic peculiarities of mink DNA distribution in the hybridoma cell lines had any bearing upon the secretion or non-secretion of mink Ig. There was no synthesis of lambda-L-chains of mink Ig in line 7 cells because the line lost the lambda-gene. With the aid of in situ hybridization with 3H-labeled total mink DNA, a considerable transformation of hybridoma cell karyotype was observed. Multiple integration of the mink DNA into mouse chromosomes and the appearance of chromosomes not characteristic for either the mink or mouse parent cells were noted. Increasing numbers of cells with translocations of mink chromosomes fragments into mouse chromosomes were found in the hybridoma lines cultivated for lengthy periods.

Review: Veterinary Sources of Nonrodent Monoclonal Antibodies: Interspecific and Intraspecific Hybridomas

“…(44) MAbs have been successfully derived from all of the common Western domestic species, including cattle, (45)(46)(47)(48) sheep, (20,49,50) goats, (51,52) pigs, (53)(54)(55) and horses (56,57) as well as cats, (58,59) dogs, (60,61) and mink. (62,63) These studies have been extensions of ongoing work on polyclonal antibodies or investigations into particular applications requiring nonmurine antibody-secreting lines. Pig (54) and sheep MAbs (23) have both been suggested as candidates for human immunotherapy.…”