Miniaturised hybrid immunoassay for high sensitivity analysis of aflatoxin M1 in milk

Kanungo, Lizy; Pal, Souvik; Bhand, Sunil

doi:10.1016/j.bios.2010.11.014

Cited by 42 publications

(24 citation statements)

References 25 publications

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“…Regarding the method used for the identification of AFM 1 in bovine milk, it showed a good sensitivity and precision for the quantification of aflatoxin within the Brazilian parameters, although there are also very sensitive methods for detection of aflatoxin M1 (Vdovenko et al, 2014;Kanungo et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

Determination of aflatoxin M1 in bovine milk from the Alagoas/Brazil State dairy belt by high performance liquid chromatography (HPLC)

Alves¹,

Lima²,

Costa³

et al. 2018

Afr. J. Microbiol. Res.

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Milk is considered a nutritionally noble food and is therefore suitable for feeding children and adults. However, contamination of milk by mycotoxins may pose a health risk to the consumer. Aflatoxins, mycotoxins produced by fungi of the genus Aspergillus, can be found in several food products, including milk and its derivatives, which reinforces the importance of this type of study on the occurrence of the aflatoxin M 1 (AFM 1 ) in raw milk. We analyzed 45 samples of raw bovine milk from expansion tanks from 23 farms in different municipalities of the dairy belt of the state of Alagoas/Brazil. Samples were collected directly from the cooling tanks and transported under refrigeration for analysis. The method used for the extraction of AFM 1 was that proposed by the Adolfo Lutz Institute. On the other hand, the detection of AFM 1 occurred by high performance liquid chromatography (HPLC) by identifying retention times. The results of the analyses indicated that none of the collected samples presented contamination by aflatoxin M 1 , thus indicating that the milk commercialized in Alagoas shows a good quality against this toxic agent.

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Section: Resultsmentioning

confidence: 99%

Determination of aflatoxin M1 in bovine milk from the Alagoas/Brazil State dairy belt by high performance liquid chromatography (HPLC)

Alves¹,

Lima²,

Costa³

et al. 2018

Afr. J. Microbiol. Res.

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show abstract

“…The author reported a LOD of 30 pg/mL with the high throughput ELISA procedure. In the last decades, nanomaterial-based signal amplification strategies for conventional ELISA and electrochemical detection have been applied for AFM1 detection [53,54]. For AFB1 detection, an impedimetric immunosensor based on colloidal gold and silver electrodeposition for AFM1 detection was developed by Vig et al [55].…”

Section: Electrochemical Immunosensors For Afs Detectionsmentioning

confidence: 99%

Recent Advances in Electrochemical-Based Sensing Platforms for Aflatoxins Detection

et al. 2016

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Abstract:Mycotoxin are small (MW~700 Da), toxic secondary metabolites produced by fungal species that readily colonize crops and contaminate them at both pre-and post-harvesting. Among all, aflatoxins (AFs) are mycotoxins of major significance due to their presence in common food commodities and the potential threat to human health worldwide. Based on the severity of illness and increased incidences of AFs poisoning, a broad range of conventional and analytical detection techniques that could be useful and practical have already been reported. However, due to the variety of structural analogous of these toxins, it is impossible to use one common technique for their analysis. Numerous recent research efforts have been directed to explore alternative detection technologies. Recently, immunosensors and aptasensors have gained promising potential in the area of sample preparation and detection systems. These sensors offer the advantages of disposability, portability, miniaturization, and on-site analysis. In a typical design of an aptasensor, an aptamer (ssDNA or RNA) is used as a bio-recognition element either integrated within or in intimate association with the transducer surface. This review paper is focused on the recent advances in electrochemical immunoand aptasensing platforms for detection of AFs in real samples.

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“…The hybrid-assay, by coupling the 1 antibodies (Ab) immobilized MNPs column with microwell plate assay enabled simultaneous measurement of low (0.5 pg/mL) and high AFM1 contamination (200 pg/mL). The MNPs-ELISA advantages were that it had a small column size, high capture efficiency and lower cost over other reported materials [46].…”

Section: Update On Aflatoxin M1 Extraction Detection and Quantificatmentioning

confidence: 99%

“…This method showed a 2% of false negative results. Kanungo et al [46] also developed an ultra-sensitive sandwich for the detection of AFM1 in milk. The assay involved the immobilization of rat monoclonal antibody of AFM1 in 384 microtiter plate to capture AFM1 antigen.…”

Section: Update On Aflatoxin M1 Extraction Detection and Quantificatmentioning

confidence: 99%

“…The assay involved the immobilization of rat monoclonal antibody of AFM1 in 384 microtiter plate to capture AFM1 antigen. The miniaturised assay (10µL) enabled ultra-trace analysis of AFM1 in milk with much improved lower limit of detection at 0.005pg/mL [46]. Sensitive magnetic nanoparticles (MNPs) based ELISA has been also developed and coupled with micro plate ELISA for analysis in milk.…”

Section: Update On Aflatoxin M1 Extraction Detection and Quantificatmentioning

confidence: 99%

See 1 more Smart Citation

A Decade of Aflatoxin M1 Surveillance in Milk and Dairy Products in Developing Countries (2001-2011): A Review

Mwanza¹,

Ngoma²,

Nyirenda³

et al. 2013

Mycotoxin and Food Safety in Developing Countries

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Miniaturised hybrid immunoassay for high sensitivity analysis of aflatoxin M1 in milk

Cited by 42 publications

References 25 publications

Determination of aflatoxin M1 in bovine milk from the Alagoas/Brazil State dairy belt by high performance liquid chromatography (HPLC)

Determination of aflatoxin M1 in bovine milk from the Alagoas/Brazil State dairy belt by high performance liquid chromatography (HPLC)

Recent Advances in Electrochemical-Based Sensing Platforms for Aflatoxins Detection

A Decade of Aflatoxin M1 Surveillance in Milk and Dairy Products in Developing Countries (2001-2011): A Review

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