Recent Advances in Electrochemical-Based Sensing Platforms for Aflatoxins Detection

Abstract

Abstract:Mycotoxin are small (MW~700 Da), toxic secondary metabolites produced by fungal species that readily colonize crops and contaminate them at both pre-and post-harvesting. Among all, aflatoxins (AFs) are mycotoxins of major significance due to their presence in common food commodities and the potential threat to human health worldwide. Based on the severity of illness and increased incidences of AFs poisoning, a broad range of conventional and analytical detection techniques that could be useful and practical have already been reported. However, due to the variety of structural analogous of these toxins, it is impossible to use one common technique for their analysis. Numerous recent research efforts have been directed to explore alternative detection technologies. Recently, immunosensors and aptasensors have gained promising potential in the area of sample preparation and detection systems. These sensors offer the advantages of disposability, portability, miniaturization, and on-site analysis. In a typical design of an aptasensor, an aptamer (ssDNA or RNA) is used as a bio-recognition element either integrated within or in intimate association with the transducer surface. This review paper is focused on the recent advances in electrochemical immunoand aptasensing platforms for detection of AFs in real samples.

Thin Films Sensor Devices for Mycotoxins Detection in Foods: Applications and Challenges

“…To transform the mycotoxin interaction into a measurable analytical signal, different electrochemical techniques have been used like (1) amperometric, which measures the changes in the current at a given applied voltage resulting from the oxidation or reduction of an electroactive biological element providing specific quantitative analytical information; (2) potentiometric, which measures the changes in the voltage between the working and the reference electrodes due to the establishment of an electrostatic interaction; (3) conductometric, which measures the changes in the capability of the sensing material to transport charge (electron); and (4) impedimetric, which measures the resistance of the generated electric current at certain applied voltage and combines the analysis of both the resistive and capacitive properties of the materials [109][110][111][112][113]. Many review articles have focused on mycotoxin detection using different electrochemical biosensors [47,107,114,115] and on the application of nanomaterial-mediated bio and immunosensors [21,75,76]. Table 3 summarizes research regarding electrochemical sensors devices recently used for mycotoxin analysis in food samples.…”

Electrochemical Immunosensors and Aptasensors

“…The review by Marty and coworkers [21] presents the state-of-the-art in the field of aflatoxin (AF) detection with affinity biosensors. Mycotoxins are toxic secondary metabolites produced by fungal species that can colonize and contaminate crops.…”

Electrochemical Aptasensor Based on Poly(Neutral Red) and Carboxylated Pillar[5]arene for Sensitive Determination of Aflatoxin M1

“…Several approaches to the sensor based determination of mycotoxins have been reported in the past decade (see reviews ). Thus, indirect competitive immunoassay has been elaborated with screen‐printed electrode array adapted to use together with a standard 96‐well microplate for the immunoassay of frozen and lyophilized milk .…”