Mineralization Mechanism of Calcium Phosphates under Three Kinds of Langmuir Monolayers

Zhang, Lijuan; Liu, Hong-Guo; Feng, Xi; Zhang, Ren‐Jie; Zhang, Li; Ye, Mu; Hao, Jingcheng; Qian, Dong−Jin; Lou, Yiting

doi:10.1021/la035381j

Cited by 50 publications

(64 citation statements)

References 51 publications

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“…The control titanium sample and the OH-modified sample showed less calcium phosphate deposition than the COOH-and PO 4 H 2 -modified samples [52]. While most of the results so far indicate that negatively charged functional groups are more effective than positive or neutral groups in inducing heterogeneous nucleation of HA [50,53], there are some studies showing different results: Zhang et al [60] investigated HA precipitation in the presence of PO 4 , COOH and NH 2 functional groups present on Langmuir monolayers made of dipalmitoylphosphatidylcholine, arachidic acid and octadecylamine, respectively. They showed that all PO 4 , COOH and NH 2 functional groups promoted the nucleation of calcium phosphate to very similar extents [60].…”

Section: Amino Acids Bound To Surfacesmentioning

confidence: 99%

“…While most of the results so far indicate that negatively charged functional groups are more effective than positive or neutral groups in inducing heterogeneous nucleation of HA [50,53], there are some studies showing different results: Zhang et al [60] investigated HA precipitation in the presence of PO 4 , COOH and NH 2 functional groups present on Langmuir monolayers made of dipalmitoylphosphatidylcholine, arachidic acid and octadecylamine, respectively. They showed that all PO 4 , COOH and NH 2 functional groups promoted the nucleation of calcium phosphate to very similar extents [60]. However, the Ca/P ratio for the calcium phosphates formed in the presence of PO 4 was similar to that of HA (1.67), whereas COOH (1.49) and NH 2 (1.60) showed lower Ca/P ratios indicating the presence of ACP precursor.…”

Section: Amino Acids Bound To Surfacesmentioning

confidence: 99%

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The role of amino acids in hydroxyapatite mineralization

Tavafoghi¹,

Cerruti²

2016

J. R. Soc. Interface.

199

147

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Polar and charged amino acids (AAs) are heavily expressed in noncollagenous proteins (NCPs), and are involved in hydroxyapatite (HA) mineralization in bone. Here, we review what is known on the effect of single AAs on HA precipitation. Negatively charged AAs, such as aspartic acid, glutamic acid (Glu) and phosphoserine are largely expressed in NCPs and play a critical role in controlling HA nucleation and growth. Positively charged ones such as arginine (Arg) or lysine (Lys) are heavily involved in HA nucleation within extracellular matrix proteins such as collagen. Glu, Arg and Lys intake can also increase bone mineral density by stimulating growth hormone production. In vitro studies suggest that the role of AAs in controlling HA precipitation is affected by their mobility. While dissolved AAs are able to inhibit HA precipitation and growth by chelating Ca 2þ and PO 4 32 ions or binding to nuclei of calcium phosphate and preventing their further growth, AAs bound to surfaces can promote HA precipitation by attracting Ca 2þ and PO 4 32 ions and increasing the local supersaturation. Overall, the effect of AAs on HA precipitation is worth being investigated more, especially under conditions closer to the physiological ones, where the presence of other factors such as collagen, mineralization inhibitors, and cells heavily influences HA precipitation. A deeper understanding of the role of AAs in HA mineralization will increase our fundamental knowledge related to bone formation, and could lead to new therapies to improve bone regeneration in damaged tissues or cure pathological diseases caused by excessive mineralization in tissues such as cartilage, blood vessels and cardiac valves.

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Section: Amino Acids Bound To Surfacesmentioning

confidence: 99%

Section: Amino Acids Bound To Surfacesmentioning

confidence: 99%

The role of amino acids in hydroxyapatite mineralization

Tavafoghi¹,

Cerruti²

2016

J. R. Soc. Interface.

199

147

View full text Add to dashboard Cite

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“…Most of the works show that HA precipitation was faster on negatively charged than on positively charged SAMs, and that among the negatively charged groups, phosphonate groups are the strongest HA nucleators [23,24]. In one case, though, it was found that PO 4 , COOH and NH 2 functional groups promoted the nucleation of calcium phosphate to a very similar extent [25]. However, the Ca/P ratio for the calcium phosphate formed in the presence of PO 4 was similar to that of HA (1.67) while lower Ca/P ratios were found for COOH (1.49) and NH 2 (1.60), indicating the presence of some amorphous calcium phosphate (ACP) on these samples.…”

Section: Introductionmentioning

confidence: 99%

Hydroxyapatite formation on graphene oxide modified with amino acids: arginine versus glutamic acid

Tavafoghi¹,

Brodusch²,

Gauvin³

et al. 2016

J. R. Soc. Interface.

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Hydroxyapatite (HA, Ca 5 (PO 4 ) 3 OH) is the main inorganic component of hard tissues, such as bone and dentine. HA nucleation involves a set of negatively charged phosphorylated proteins known as non-collagenous proteins (NCPs). These proteins attract Ca 2þ and PO 4 32 ions and increase the local supersaturation to a level required for HA precipitation. Polar and charged amino acids (AAs) are highly expressed in NCPs, and seem to be responsible for the mineralizing effect of NCPs; however, the individual effect of these AAs on HA mineralization is still unclear. In this work, we investigate the effect of a negatively charged (Glu) and positively charged (Arg) AA bound to carboxylated graphene oxide (CGO) on HA mineralization in simulated body fluids (SBF).Our results show that Arg induces HA precipitation faster and in larger amounts than Glu. We attribute this to the higher stability of the complexes formed between Arg and Ca 2þ and PO 4 32 ions, and also to the fact that Arg exposes both carboxyl and amino groups on the surface. These can electrostatically attract both Ca 2þ and PO 4 32 ions, thus increasing local supersaturation more than Glu, which exposes carboxyl groups only.

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“…(-NH 3+ ) groups, this organic multilayered template is able to regulate biomineral formation and overcome the dispersion problem of CaP particles on PET fibers (17). In addition, the carboxylic groups interact with the calcium ions of CaP, which enables the multilayered template to effectively stimulate BM and induce the deposition of crystalline CaP (18)(19)(20). However, in contrast to other fast methods for biomimetic deposition of nanocrystalline apatite, the current technique requires approximately three days to deposit a uniform nanoscale CaP coating on the PET ligament, which may be due to the chemical and physical properties of the surface of the PET ligament.…”

Section: Resultsmentioning

confidence: 99%

Preparation and in vitro evaluation of a biomimetic nanoscale calcium phosphate coating on a polyethylene terephthalate artificial ligament

Chen

Guo

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. In the present study, a polyethylene terephthalate (PET) artificial ligament was coated with an organic layer-by-layer (LBL) self-assembled template of chitosan and hyaluronic acid, and then incubated in a calcium phosphate (CaP) solution to prepare a biomimetic CaP coating. The surface characterization of the ligament was examined using scanning electron microscopy, atomic force microscopy and energy-dispersive X-ray spectroscopy. The effects of CaP coatings on the osteogenic activity of MC3T3 E1 mouse osteoblastic cells were investigated by evaluating their attachment, proliferation and the relative expression levels of alkaline phosphatase. The results revealed that the organic LBL template on the PET artificial ligament was effective for CaP apatite formation. Following incubation for 72 h, numerous nanoscale CaP apatites were deposited on the PET ligament fibers. In addition, the results of the in vitro culture of MC3T3-E1 mouse osteoblastic cells demonstrated that the CaP coating had a good biocompatibility for cell proliferation and adhesion, and the CaP-coated group had a significantly higher alkaline phosphatase activity compared with the uncoated control group after seven days of cell culture. Collectively, these results demonstrated that the biomimetic nanoscale CaP-coated PET artificial ligaments have potential in bone-tissue engineering.

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Mineralization Mechanism of Calcium Phosphates under Three Kinds of Langmuir Monolayers

Cited by 50 publications

References 51 publications

The role of amino acids in hydroxyapatite mineralization

The role of amino acids in hydroxyapatite mineralization

Hydroxyapatite formation on graphene oxide modified with amino acids: arginine versus glutamic acid

Preparation and in vitro evaluation of a biomimetic nanoscale calcium phosphate coating on a polyethylene terephthalate artificial ligament

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