Preparation and in vitro evaluation of a biomimetic nanoscale calcium phosphate coating on a polyethylene terephthalate artificial ligament

Abstract

Abstract. In the present study, a polyethylene terephthalate (PET) artificial ligament was coated with an organic layer-by-layer (LBL) self-assembled template of chitosan and hyaluronic acid, and then incubated in a calcium phosphate (CaP) solution to prepare a biomimetic CaP coating. The surface characterization of the ligament was examined using scanning electron microscopy, atomic force microscopy and energy-dispersive X-ray spectroscopy. The effects of CaP coatings on the osteogenic activity of MC3T3 E1 mouse osteoblastic cells were investigated by evaluating their attachment, proliferation and the relative expression levels of alkaline phosphatase. The results revealed that the organic LBL template on the PET artificial ligament was effective for CaP apatite formation. Following incubation for 72 h, numerous nanoscale CaP apatites were deposited on the PET ligament fibers. In addition, the results of the in vitro culture of MC3T3-E1 mouse osteoblastic cells demonstrated that the CaP coating had a good biocompatibility for cell proliferation and adhesion, and the CaP-coated group had a significantly higher alkaline phosphatase activity compared with the uncoated control group after seven days of cell culture. Collectively, these results demonstrated that the biomimetic nanoscale CaP-coated PET artificial ligaments have potential in bone-tissue engineering.

Enhance the biocompatibility and osseointegration of polyethylene terephthalate ligament by plasma spraying with hydroxyapatite in vitro and in vivo

“…5 Synthetic materials for ACL reconstruction and modification of those materials have been investigated for their potential to address the challenges of osseointegration. [6][7][8][9] Our previous studies have applied gelatin, 10 silk fibroin, 11 and material based on calcium phosphate 12 to artificial ligament using different surface modification techniques, which have shown positive effect on the biological performance. Among these materials, HA has been proved to improve osseointegration after applied on PET ligament.…”

“…Among these materials, HA has been proved to improve osseointegration after applied on PET ligament. 12 HA, which is the natural component of bone tissue, has been studied in a series of researches for implant surface modification. [13][14][15] Multiple studies have investigated the biological performance and the material characteristics of HA-based surface modification.…”

Strontium-Doped Hydroxyapatite Coating Improves Osteo/Angiogenesis for Ameliorative Graft-Bone Integration via the Macrophage-Derived Cytokines-Mediated Integrin Signal Pathway

“…As a result, HA is known for its exceptional osteoconductive and osteoinductive properties. 14,15 However, the biodegradation of HA and the subsequent restoration of bone functions pose significant challenges. 16 In addressing these issues, researchers have proposed including metallic elements such as strontium (Sr), silicon, and magnesium ions in repair materials to enhance their mechanical properties.…”

Biointegration of anterior cruciate ligament protesis made of polyethylene tetraftalate in late postoperation period

“…История синтетических протезов связок эволюционировала от углеродных волокон [6] до современных LARS на основе полиэтилен тетрафталата. Изначально полимерные имплантаты ПКС давали достаточно большое количество осложнений, например синовит коленного сустава [1,14], были хрупкими, часто повреждались о край костного канала, в котором располагались [15,16]. Это заставляло искать полимерные материалы и покрытия без вышеуказанных недостатков.…”

“…Так были синтезированы полимерные протезы ПКС на основе ПЭТ. Эти имплантаты обладали достаточными прочностными характеристиками, которые обеспечивали раннюю нагрузку на конечность и показывали неплохие результаты в клинической практике [7,9,15]. Однако оценка эффективности выполненных операций проводилась только на основании клинических результатов, вопрос биоинтеграции полимеров на основе ПЭТ оставался открытым.…”